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  • Book
    edited by Myron Christodoulides.
    Contents:
    Neisseria meningitidis: biology, microbiology, and epidemiology
    Classification and pathogenesis of meningococcal infections
    Detection of Neisseria meningitidis in cerebrospinal fluid using a multiplex PCR and the luminex detection technology
    Generating knock-out and complementation strains of Neisseria meningitidis
    Identification and functional characterization of sRNAs in Neisseria meningitidis
    Expression, purification, and crystallization of neisserial outer membrane proteins
    Genome-scale metabolic models: reconstruction and analysis
    TMT labelling for the quantitative analysis of adaptive responses in the meningococcal proteome
    Meningococcal ligands and molecular targets of the host
    In vivo imaging of meningococcal disease dynamics
    Methods for studying Neisseria meningitidis biofilms
    Laminar-flow chamber assay for measuring bacterial adhesion under shear stress
    Techniques to measure pilus retraction forces
    Human dendritic cell culture and bacterial infection
    Hydrogen-deuterium exchange coupled to mass spectrometry to investigate ligand-receptor interactions
    Visualising PAMP-PRR interactions using nanoscale imaging
    Transcriptome analyses in the interaction of Neisseria meningitidis with mammalian host cells
    Use of the pan-Neisseria microarray and experimental design for transcriptomics studies of Neisseria
    Analysis of parameters associated with prevention of cellular apoptosis by pathogenic Neisseriae and purified porins
    Analysis of the immune response to Nia meningitidis using a proteomics approach
    Antigen identification starting from the genome: a "reverse vaccinology" approach applied to MenB
    DNA vaccine strategy for effective antibody induction to pathogen-derived antigens.
    Digital Access Springer 2012
  • Article
    Shadduck RK, Waheed A.
    Blood Cells. 1979 Aug;5(3):421-34.
    Purified L-cell colony stimulating factor (CSF) and rabbit anti-CSF serum were used to devise a radioimmunoassay for this factor. The CSF was radiolabelled with the aid of lactoperoxidase and precipitated by a double antibody technique. Addition of unlabelled CSF caused a dose-related displacement of the labelled tracer. Similar results were noted with conditioned media and murine serum. The assay required only 4 days for completion as compared with 7 days for the conventional agar gel bioassay. Moreover, the radioimmunoassay proved more sensitive and accurate than the bioassay. This technique should allow further exploration of the role of CSF in granulopoiesis.
    Digital Access Access Options