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  • Book
    edited by Irina Artsimovitch, Thomas J. Santangelo.
    Contents:
    Mapping the Escherichia coli transcription elongation complex with exonuclease III / Zhaokun Liu and Irina Artsimovitch
    Purification of bacterial RNA polymerase : tools and protocols / Vladimir Svetlov and Irina Artsimovitch
    Monitoring translocation of multisubunit RNA polymerase along the DNA with fluorescent base analogues / Anssi M. Malinen, Matti Turtola, and Georgiy A. Belogurov
    In vitro and in vivo methodologies for studying the sigma-54-dependent transcription / Martin Buck [and nine others]
    Methods for the assembly and analysis of in vitro transcription-coupled-to-translation systems / Daniel Castro-Roa and Nikolay Zenkin
    Site-specific incorporation of probes into RNA polymerase by unnatural-amino-acid mutagenesis and Straudinger-Bertozzi ligation / Anirban Chakraborty [and seven others]
    Reconstruction of factor-dependent, promotor proximal pausing in Drosophila nuclear extracts / Jian Li and David S. Gilmour
    Direct competition assay for transcription fidelity / Lucyna Lubkowska and Maria L. Kireeva
    Single-stranded DNA aptamers for functional probing of bacterial RNA polymerase / Danil Pupov and Andrey Kulbachinskiy
    Biochemical analysis of transcription termination by RNA polymerase III from yeast Saccharomyces cerevisiae / Aneeshkumar G. Arimbasseri and Richard J. Maraia
    Use of RNA polymerase molecular beacon assay to measure RNA polymerase interactions with model promotor fragments / Vladimir Mekler and Konstantin Severinov
    Preparation of cDNA libraries for high-throughput RNA sequencing analysis of RNA 5' ends / Irina O. Vvedenskaya, Seth R. Goldman, and Bryce E. Nickels
    In situ footprinting of E. coli transcription elongation complex with chloroacetaldehyde / A. Rachid Rahmouni and Christine Mosrin-Hauman
    Using solutes and kinetics to probe large conformational changes in the steps of transcription initiation / Emily F. Ruff, Wayne S. Kontur, and M. Thomas Record Jr.
    Manipulating archaeal systems to permit analyses of transcription elongation-termination decisions in vitro / Alexandra M. Gehring and Thomas J. Santangelo
    Purification of active RNA polymerase I from yeast / Francis Dean Appling and David Alan Schneider
    Transcription in archaea : preparation of Methanocaldococcus jannaschii transcription machinery / Katherine Smollet, Fabian Blombach, and Finn Werner
    Transcription in archaea ; in vitro transcription assays for mjRNAP / Katherine Smollet, Fabian Blombach, and Finn Werner
    Experimental analysis of hFACT action during pol II transcription in vitro / Fu-Kai Hsieh, Olga I. Kulaeva, and Vasily M. Studitsky
    ChIP-Seq for genome-scale analysis of bacterial DNA-binding proteins / Richard P. Bonocora and Joseph T. Wade.
    Digital Access Springer 2015
  • Article
    Maruyama Y, Coleman MS, Bullock WE.
    Cancer Biochem Biophys. 1979;3(3):143-50.
    The effects of Corynebacterium parvum on the lymphohemopoietic tissues of mice was investigated by H3-TdR metabolism, organ weights, DNA-polymerase-alpha activity and terminal deoxynucleotidyl transferase (TdT) activity. Marked increase in spleen size occurred. The increase in size was accompanied by increases in DNA-P-alpha activity and H3TdR uptake. This indicated that the spleen was a major site of cell proliferation and of increase in population size after C. parvum stimulation. Thymus and bone marrow changes are also described. The thymus showed a marked decrease in size which was maximal in 10 days and showed recovery thereafter. Thymus TdT activity fell immediately and may reflect either a release or lysis of thymocytes which contain TdT activity. However, no change in TdT activity was measurable in the bone marrow or spleen. The principal cell population increase had phagocytic activity and was presumably monocytes-macrophages, and an increase in such cells was found in the spleen.
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