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- Bookedited by Gianfranco Donelli.Contents:
Methods for dynamic investigations of surface-attached in vitro bacterial and fungal biofilms / Claus Sternberg, Thomas Bjarnsholt, and Mark Shirtliff
Aqueous two-phase system technology for patterning bacterial communities and biofilms / Mohammed Dwidar, Shuichi Takayama, and Robert J. Mitchell
Quorum sensing in gram-positive bacteria : assay protocols for Staphylococcal agr and Enterococcal fsr systems / Akane Shojima and Jiro Nakayama
Advanced techniques for in situ analysis of the biofilm matrix (structure, composition, dynamics) by means of laser scanning microscopy / Thomas R. Neu and John R. Lawrence
Multiplex fluorescence in situ hybridization (M-FISH) and confocal laser scanning microscopy (CLSM) to analyze multispecies oral biofilms / Lamprini Karygianni, Elmar Hellwig, and Ali Al-Ahmad
Field emission scanning electron microscopy of biofilm-growing bacteria Involved in nosocomial infections / Claudia Vuotto and Gianfranco Donelli
Experimental approaches to investigating the vaginal biofilm microbiome / Marc M. Baum, Manjula Gunawardana, and Paul Webster
Imaging bacteria and biofilms on hardware and periprosthetic tissue in orthopedic infections / Laura Nistico, Luanne Hall-Stoodley, and Paul Stoodley
Animal models to evaluate bacterial biofilm development / Kim Thomsen, Hannah Trøstrup, and Claus Moser
Animal models to investigate fungal biofilm formation / Jyotsna Chandra, Eric Pearlman, and Mahmoud A. Ghannoum
Nonmammalian model systems to investigate fungal biofilms / Marios Arvanitis ... [et al.]
Microbiological methods for target-oriented screening of biofilm inhibitors / Livia Leoni and Paolo Landini
In vitro screening of antifungal compounds able to counteract biofilm development / Marion Girardot and Christine Imbert
Biofilm matrix-degrading enzymes / Jeffrey B. Kaplan
Efficacy evaluation of antimicrobial drug-releasing polymer matrices / Iolanda Francolini, Antonella Piozzi, and Gianfranco Donelli
Antibiotic polymeric nanoparticles for biofilm-associated infection therapy / Wean Sin Cheow and Kunn Hadinoto
Pharmacokinetics and pharmacodynamics of antibiotics in biofilm infections of Pseudomonas aeruginosa in vitro and in vivo / Wang Hengzhuang, Niels Høiby, and Oana Ciofu
Contribution of confocal laser scanning microscopy in deciphering biofilm tridimensional structure and reactivity / Arnaud Bridier and Romain Briandet
Chip calorimetry for evaluation of biofilm treatment with biocides, antibiotics, and biological agents / Frida Mariana Morais, Friederike Buchholz, and Thomas Maskow
Bacteriophage attack as an anti-biofilm strategy / Sanna Sillankorva and Joana Azeredo
Photodynamic therapy as a novel antimicrobial strategy against biofilm-based nosocomial infections : study protocols / Francesco Giuliani
Capturing air-water interface biofilms for microscopy and molecular analysis / Margaret C. Henk
Biofilm-growing bacteria involved in the corrosion of concrete wastewater pipes : protocols for comparative metagenomic analyses/ Vicente Gomez-Alvarez
Culture-independent methods to study subaerial biofilm growing on biodeteriorated surfaces of stone cultural heritage and frescoes / Francesca Cappitelli, Federica Villa, and Andrea Polo
Biofilms of thermophilic bacilli isolated from dairy processing plants and efficacy of sanitizers / Sara A. Burgess, Denise Lindsay, and Steve H. Flint.Digital Access Springer 2014 - ArticleShichi H, Williams TC.J Supramol Struct. 1979;12(4):419-24.Frogs (Rana pipiens) were injected subcutaneously with (3H)-leucine and allowed to incorporate the radioactive amino acid into newly assembled disks in the retinal rod outer segment. The labeled disks served as a temporal marker for following the turnover of rod outer segments. Animals were killed at different times after injection and outer segments were isolated and phosphorylated with ATP in the light. The visual pigment (as isorhodopsin) was regenerated with 9-cis retinal, extracted, and chromatographed on epichlorohydrin triethanolamine cellulose so that phosphorylated pigment could be separated from unphosphorylated pigment. The ratio of (3H)-radioactivity of phosphorylated pigment to that of unphosphorylated pigment was then plotted against the time after injection. The ratio was high when (3H)-labeled disks were largely associated with the basal region of the rod and decreased as the labeled disks moved toward the rod apical region. The results were interpreted as suggesting that newer disks are phosphorylated preferentially to older disks. Papain digestion of (3H)-labeled disks indicated that rhodopsin in newer disks is more susceptible to proteolysis than that in older disks.