Bookedited by Svein Valla and Rahmi Lale.
Contents:
1. BioBrick assembly standards and techniques and associated software tools / Gunvor Røkke, Eirin Korvald, Jarle Pahr, Ove Øyås and Rahmi Lale
2. Plasmid construction by SLIC or sequence and ligation- independent cloning / Ryan E. Hill and Julian J. Eaton-Rye
3. Quick and clean cloning / Frank Thieme and Sylvestre Marillonnet
4. Hierarchical ligation-independent assembly of PCR fragments / Jonathan L. Schmid-Burgk, Zhen Xie, and Yaakov Benenson
5. USER-derived cloning methods and their primer design / Bo Salomonsen, Uffe H. Mortensen, and Barbara A. Halkier
6. Application of the restriction-free (RF) cloning for multicomponents assembly / Yoav Peleg and Tamar Unger
7. A single-tube assembly of DNA using the transfer-PCR (TPCR) platform / Ariel Erijman, Julia M. Shifman, and Yoav Peleg
8. Circular polymerase extension cloning / Jiayuan Quan and Jingdong Tian
9. Golden gate cloning / Carola Engler and Sylvestre Marillonnet
10. Design and construction of multigenic constructs for plant biotechnology using the goldenbraid cloning strategy / Alejandro Sarrion-Perdigones, Jorge Palaci, Antonio Granell and Diego Orzaez
11. FX cloning : a simple and robust high-throughput cloning method for protein expression / Eric R. Geertsma
12. Minimum GC-rich sequences for overlap extension PCR and primer annealing / Mikiko Nakamura, Ayako Suzuki, Hisashi Hoshida and Rinji Akada
13. Simple cloning and DNA assembly in Escherichia coli by prolonged overlap extension PCR / Chun You and Y.-H. Percival Zhang
14. Combinatorial assembly of clone libraries using site-specific recombination / Vanessa E. Wall, Leslie A. Garvey, Jennifer L. Mehalko, Lauren V. Procter and Dominic Esposito
15. Application of In-Fusion [trademark] cloning for the parallel construction of E. coli expression vectors / Louise E. Bird, Heather Rada, John Flanagan, Jonathan M. Diprose, Robert J.C. Gilbert and Raymond J. Owens
16. Seamless ligation cloning extract (SLiCE) cloning method / Yongwei Zhang, Uwe Werling, and Winfried Edelmann
17. j5 DNA assembly design automation / Nathan J. Hillson
18. FastPCR software for PCR, in silico PCR, and oligonucleotide assembly and analysis / Ruslan Kalendar, David Lee, and Alan H. Schulman.