Bookedited by José Luis Riechmann, Frank Wellmer.
Summary: In Flower Development: Methods and Protocols, researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Flower Development: Methods and Protocols ?is an essential guide for plant developmental biologists, from the novice to the experienced researcher, and for those considering venturing into the field.
Contents:
Flower Development in Arabidopsis - There is More to it than Learning your ABCs
Flower Development in the Asterid Lineage
Grass Flower Development
Flower Diversity and Angiosperm Diversification
Flower Development - Open Questions and Future Directions
Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors
Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development
Genetic and Phenotypic Analyses of Petal Development in Arabidopsis
Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice
Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana
Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis
Microscopic Analysis of Arabidopsis Ovules
Scanning Electron Microscopy Analysis of Floral Development
Detection of mRNA Expression Patterns by Non-radioactive in situ Hybridization on Histological Sections of Floral Tissue
The GUS Reporter System in Flower Development Studies
A Floral Induction System for the Study of Early Arabidopsis Flower Development
Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell Types
Translating Ribosome Affinity Purification (TRAP) for Cell-specific Translation Profiling in Developing Flowers
Laser-assisted Microdissection Applied to Floral Tissues
Identification of Arabidopsis Knock-out Lines for Genes of Interest
Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues
Misexpression Approaches for the Manipulation of Flower Development
Next Generation Sequencing Applied to Flower Development: RNA-Seq
Next-Generation Sequencing Applied to Flower Development: ChIP-Seq
Live-Imaging of the Arabidopsis Inflorescence Meristem
Gene Regulatory Network Models for Floral Organ Determination.