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  • Book
    edited by Svetlana Makovets.
    Contents:
    DNA electrophoresis: historical and theoretical perspectives / Gareth A. Roberts and David T.F. Dryden
    Basic DNA electrophoresis in molecular cloning: a comprehensive guide for beginners / Svetlana Makovets
    Analysis of branched DNA replication and recombination intermediates from prokaryotic cells by two-dimensional (2D) native-native agarose gel electrophoresis / Nicholas P. Robinson
    Analysis of DNA structures from eukaryotic cells by two-dimensional native-native DNA agarose gel electrophoresis / Andreas S. Ivessa
    Directionality of replication fork movement determined by two-dimensional native-native DNA agarose gel electrophoresis / Andreas S. Ivessa
    Native/denaturing two-dimensional DNA electrophoresis and its application to the analysis of recombination intermediates / Jessica P. Lao, Shangming Tang, and Neil Hunter
    Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis / Jorge B. Schvartzman [and three others]
    A neutral glyoxal gel electrophoresis method for the detection and semi-quantitation of DNA single-strand breaks / Brian Pachkowski and Jun Nakamura
    Denaturing gradient gel electrophoresis (DGGE) / Fiona Strathdee and Andrew Free
    Polyacrylamide temperature gradient gel electrophoresis / Viktor Viglasky
    Separation of DNA oligonucleotides using denaturing urea PAGE / Fiona Flett and Heidrun Interthal
    Pulsed-field gel electrophoresis of bacterial chromosomes / Julia S.P. Mawer and David R.F. Leach
    Resolution of budding yeast chromosomes using pulsed-field gel electrophoresis / Aziz El Hage and Jonathan Houseley
    Analysis of DNA damage via single-cell electrophoresis / Diana Anderson and Julian Laubenthal
    Fluorescence in situ hybridization on electrophoresed cells to detect sequence specific DNA damage / Julian Laubenthal and Diana Anderson
    Analysis of DNA-protein interactions using PAGE: band-shift assays / Lynn Powell
    Assaying cooperativity of protein
    DNA interactions using agarose gel electrophoresis / Tanya L. Williams and Daniel L. Levy
    DNA bending by proteins: utilizing plasmid pBendAT as a tool / Fenfei Leng
    Using PCR coupled to PAGE for detection and semiquantitative evaluation of telomerase activity / Laura Gardano.
    Digital Access Springer 2013
  • Article
    Kos WL, Loria RM, Snodgrass MJ, Cohen D, Thorpe TG, Kaplan AM.
    Infect Immun. 1979 Nov;26(2):658-67.
    Previous experiments showed that nutritionally induced hypercholesteremia in mice caused an increase in susceptibility to coxsackievirus B, with a marked suppression of cellular infiltrates in infected tissues and an increased mortality. The present studies demonstrated that a hypercholesteremic diet was associated with an inhibition in host resistance as measured by susceptibility to Listeria monocytogenes infection and the growth of two transplanted syngeneic murine tumors. Moreover, the ability of Corynebacterium parvum to induce regression of a transplanted methylcholanthrene-induced fibrosarcoma was inhibited in hypercholesteremic hosts, as was the histiocytic infiltration normally accompanying C. parvum inoculation. In contrast, the peritoneal macrophages from C. parvum-treated hypercholesteremic mice were indistinguishable from similarly treated macrophages from normal mice with respect to their in vitro tumoricidal activity and the presence of a cell surface antigen associated with activated macrophages. Hypercholesteremia was also associated with a decreased antibody response to sheep erythrocytes in vivo, but dit not appear to exert a detrimental effect on B- or T-cell blastogenesis when tested in vitro. The findings that the hypercholesteremic diet was associated with an impairment in the host immune response and increased susceptibility to viral, bacterial, and tumor cell challenge are discussed with respect to virus-lipid interactions in the pathogenesis of atherogenesis and diabetes mellitus.
    Digital Access Access Options