Bookedited by Astrid E. Cardona, Eroboghene E. Ubogu.
Summary: Chemokines constitute a large family of structurally similar cytokines that contain a signature of conserved cysteine residues joined by disulfide bridges. Binding of chemokines to specific G protein-coupled receptors followed by downstream signaling defines their biological function. Initially, chemoattraction was the key function linked to chemokines/chemokine receptors; however, in recent years, it has become clear that chemokine ligand-receptor interactions can also modulate cellular activation, survival, and proliferation, among other functions in homeostatic and diseased states. Importantly, major advances in our understanding of chemokine biology have led to chemokine receptors becoming specific therapeutic targets with great potential. In Chemokines: Methods and Protocols, expert researchers provide practical information regarding experimental models and state of the art protocols used to delineate chemokine/chemokine receptor function and their applications in health and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Chemokines: Methods and Protocols aims to reveal key protocols of functional and descriptive chemokine ligand/receptor assays that will be of practical significance to graduate students, post-doctoral fellows, trainees, and researchers in academia and industry.
Contents:
Fine balance of chemokines during disease : trafficking, inflammation, and homeostasis / Sandra M. Cardona, Jenny A. Garcia, and Astrid E. Cardona
Chemokine-dependent signaling pathways in the peripheral nervous system / Eroboghene E. Ubogu
Multispectral imaging and automated laser capture microdissection of human cortical neurons : a quantitative study of CXCR4 expression / Jonathan Pitcher [and others]
Chemokine receptors and neural stem cells / Meizhang Li
Chemokine receptor interactions with virus-like particles / Luigi Buonaguro, Maria Tagliamonte, and Maria Luisa Visciano
Chemokine receptor antagonist development / Alexandre Garin [and others]
Novel approach to quantify G-protein-coupled receptor dimerization equilibrium using bioluminescence resonance energy transfer / Irina Kufareva [and others]
Unraveling chemokine and chemokine receptor expression patterns using genetically engineered mice / Simon Yona [and others]
Zebrafish as a model to study chemokine function / Eva Kochhan and Arndt F. Siekmann
Inflammatory macrophage migration in experimental autoimmune encephalomyelitis / William J. Karpus
Visualizing chemokine-dependent T cell activation and migration in response to central nervous system infection / Monica J. Carson and Emma H. Wilson
Epigenetic regulation of chemokine/chemokine receptor expression / Anne-Marie Baird, Kenneth J. O'Byrne, and Steven G. Gray
Using fluorescent chemokine uptake to detect chemokine receptors by fluorescent activated cell sorting / Laura B. Ford, Chris A.H. Hansell, and Robert J.B. Nibbs
Live cell imaging of chemotactic dendritic cell migration in explanted mouse ear preparations / Michele Weber and Michael Sixt
Studying extracellular signaling utilizing a glycoproteomic approach : lectin blot surveys, a first and important step / Jing Cao [and others].