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• Book

  The Elbow. Third edition [3rd ed.].

  editor, Bernard F. Morrey.

  Contents:
  Part I. Exposures
  part II. Fractures and trauma
  part III. Complications of trauma
  part IV. Soft tissue injuries and management
  part V. Nonreplacement and reconstructive surgery
  part VI. Joint replacement arthroplasty
  part VII. Postoperative management.

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  Ovid

  2015

  LWW Health Library

  2015
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• Article

  On the nature of the activating enzyme of the inactive form of delta-aminolevulinate synthetase in Rhodopseudomonas spheroides.

  Inoue I, Oyama H, Tuboi S.

  J Biochem. 1979 Aug;86(2):477-82.

  The activating enzyme of the inactive form of Fraction I of delta-aminolevulinate (ALA) synthetase [EC 2.3.1.37] in Rhodopseudomonas (R.) spheroides was purified about 1,000-fold from an extract of R. spheroides cells grown anaerobically in the light. The purification of the activating enzyme was achieved by fractionating the 100,000 X g supernatant fraction of the crude extract with ammonium sulfate and acetone, followed by Sephadex G-200 chromatography, pyridoxamine phosphate-Sepharose 4B chromatography, and preparative gel electrophoresis. The final preparation of the activating enzyme still contained a minor contaminant (less than 20%) as judged by disc gel electrophoresis. The activating enzyme exhibited cystathionase [EC 4.4.1.1] activity throughout the purification. These two enzyme activities were not separated at all during any step of the purification. An apparently homogeneous preparation of cystathionase [EC 4.4.1.8] purified from rat liver also exhibited activating activity in the presence of L-cystine. It was concluded that the activating enzyme is a cystathionase.

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