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  The 5-minute urology consult. Third edition [3rd ed.]

  editor-in-chief, Leonard G. Gomella, MD, FACS, ... Show More The Bernard W. Goodwin Professor of Prostate Cancer, Chairman, Department of Urology, Sidney Kimmel Medical College, Associate Director, Jefferson Sidney Kimmel Cancer Center, Clinical Director, Jefferson Sidney Kimmel Cancer Network, Thomas Jefferson University, Philadelphia, Pennsylvania ; associate editors, Gerald Andriole, MD, FACS, [and six others].

  Contents:
  Section I. Urologic diseases and conditions
  Section II. Short topics: A to Z
  Section III. Algorithms
  Section IV. Urinalyasis and urine studies
  Section V. Alternative and complementary urologic therapies
  Section VI. Urologic drug reference
  Section VII. Reference tables.

  Digital Access Ovid 2015
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• Article

  Purification and characterization of cytosol phosphoenolpyruvate carboxykinase from bullfrog (Rana catesbeiana) liver.

  Goto Y, Shimizu J, Okazaki T, Shukuya R.

  J Biochem. 1979 Jul;86(1):71-8.

  Cytosol PEP carboxykinase has been purified to electrophoretic homogeneity from bullfrog liver homogenate. The enzyme is a single polypeptide chain with a molecular weight of approximately 72,000-75,000. The purified enzyme catalyzed oxaloacetate decarboxylation (nucleoside triphosphate-supported), phosphoenolpyruvate carboxylation, and an exchange reaction between oxaloacetate and [14C]HCO3-in the presence of ITP or CTP. Manganese is absolutely required for the enzyme-catalyzed phosphoenolpyruvate carboxylation, whereas it can be replaced by Mg2+ for the oxaloacetate decarboxylation and the exchange reaction. The optimal pH of each reaction is dependent on the divalent metal ion used. The dependence of the enzyme activity on Mn2+ is markedly different in the phosphoenolpyuvate carboxylation and the oxaloacetate decarboxylation reactions.

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  PubMed