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- BookDepartment of Gynecology and Obstetrics, The Johns Hopkins University School of Medicine ; editors, Clark T. Johnson, Jennifer L. Hallock, Jessica L. Bienstock, Harold E. Fox, Edward E. Wallach.Digital Access
- ArticleCrossed immunoelectrophoretic analysis of chromatophore membranes from Rhodopseudomonas sphaeroides.Collins ML, Mallon DE, Niederman RA.J Bacteriol. 1979 Sep;139(3):1089-92.Triton extracts of intracytoplasmic photosynthetic membranes (chromatophores) purified from Rhodopseudomonas sphaeroides were subjected to crossed immunoelectrophoresis with antiserum raised in rabbits to purified chromatophores. A total of 31 immunoprecipitates was visualized; 2 of the immunoprecipitates were identified as reduced nicotinamide adenine dinucleotide (EC 1.6.99.3) and L-lactate dehydrogenases by enzyme staining techniques. Reaction with a monospecific antiserum identified the photochemical reaction center. Photopigments were associated with a major precipitate in the pattern which was identified on the basis of immunological identity as light-harvesting bacteriochlorophyll a . protein complex. These results provide the basis for a detailed structural and functional analysis of the chromatophore membrane by crossed immunoelectrophoresis.