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  • Book
    Jason C. Gallagher, Conan MacDougall ; senior acquisitions editor, Katey Birtcher ; cover design, Michael O'Donnell.
    Contents:
    Part 1. Considerations with antibiotic therapy
    Part 2. Antibacterial drugs
    Part 3. Antimycobacterial drugs
    Part 4. Antifungal drugs
    Part 5. Antiviral drugs
    Part 6. Antiparasitic Drugs
    Appendix 1. Selected normal human flora
    Appendix 2. Spectrum of activity
    Appendix 3. Empiric regimens for common infections
    Index.
    Limited to 2 simultaneous users
  • Article
    Bolhuis RL, Schuit HR.
    Clin Exp Immunol. 1979 Feb;35(2):317-23.
    Fresh lymphocytes and frozen-stored lymphocytes were separated into E-RFC-enriched and E-RFC-depleted cell fractions by density gradient centrifugation of sheep red blood cell (SRBC) rosette-forming cells (E-RFC), since the ability to form rosettes is primarily a T cell characteristic. Subpopulations of lymphocytes were identified, demonstrating the presence of cell surface markers: T cell specific antigens (T+), receptors for SRBC on T cells (E-RFC), Fc-receptors (FcR) for IgG type antibodies, and surface Ig (sIg). Our results indicate that, although the E-RFC-depleted fraction contains virtually no cells capable of binding SRBC, there is still a considerable proportion of T cells present in that fraction, as detected with the anti-T cell antiserum. Moreover, data are presented to indicate that the E-RFC-enriched fraction does not consist exclusively of T lymphocytes. Since this separation procedure is used frequently for the identification of the nature of effector cells in cell-mediated (CMC) and antibody-dependent cellular cytotoxicity (ADCC) assays, the identification of T cells in purified lymphocyte fractions by means of SRBC rosette formation may lead to a false conclusion as to the nature of the effector cells.
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