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- ArticleKisielow P, Szkudlarek J, Wysocka M.Arch Immunol Ther Exp (Warsz). 1978;26(1-6):99-103.Preparation of antisera to alloantigens Thy-1, TL, Lyt-1 and Lyt-2 is described and the importance of adequate specificity controls indicated. The specificity and activity of antisera were characterized by trypan blue exclusion cytotoxicity assay, indirect membrane immunofluorescence and absorption using cell populations from different tissues of Thy-1, TL and Lyt congenic strains of mice. In the course of these studies new serologically detectable Lyt-1 linked alloantigen was discovered which shows wide tissue distribution.