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- Bookedited by Marluce Bibbo, David C. Wilbur.Summary: In the newly updated edition of Comprehensive Cytopathology, a team of international experts provides criteria and techniques in diagnosis, testing, and new insights in cytology. This accessible guide to diagnostic investigation and screening is ideal for daily laboratory use, taking a systematic approach to helping you understand major diagnostic criteria as well as the pitfalls and limitations of cytology. Quickly find the answers you need through a consistent chapter structure.Realize every possibility. Appropriate histopathological correlations and a consideration of the possible differential diagnosis accompany the cytological findings.
Contents:
The Cell : Basic Structure and Function, and Molecular Basis of Neoplasia
Basic Cytogenetics and the Role of Genetics in Cancer Development
Cytologic Screening Programs
Diagnostic Quality Assurance in Cytopathology
Evaluation of the Non-gynecologic Sample in Smears and Liquid-based Preparations
The Bethesda System for Reporting Cervical Cytology
Microbiology, Inflammation, and Viral Infections
Benign Proliferative Reactions, Intraepithelial Neoplasia, and Invasive Cancer of the Uterine Cervix
Glandular Neoplasms of the Uterine Cervix
Endometrial Hyperplasia and Carcinoma, Extrauterine Cancer, and Unusual Tumors
Vulva, Vagina, and Anus
Peritoneal Washings and Ovary
Respiratory Tract
Alimentary Tract (Esophagus, Stomach, Small Intestine, Colon, Rectum, Anus, Biliary Tract)
Urinary Tract
Central Nervous System
Eye
Cytology of Soft Tissue, Bone, and Skin
Pleural, Peritoneal, and Pericardial Effusions
Fine-needle Aspiration Biopsy Techniques
Fine-needle Aspiration Biopsy Techniques
Salivary Glands and Rare Head and Neck Lesions
The Bethesda System for Reporting Thyroid Cytopathology
Thyroid
Lymph Nodes : Cytomorphology and Flow Cytometry
Breast
Mediastinum
Kidneys, Adrenals, and Retroperitoneum
Liver
Pancreas
Pediatric Tumors
Effects of Therapy on Cytologic Specimens
Cytopreparatory Techniques
Digital Pathology/Telepathology
Automation in Cervical Cytology
Immunocytochemistry
Molecular Techniques
HPV Detection Techniques.Digital Access ClinicalKey 2015 - ArticleCell surface changes in capping studied by correlated fluorescence and scanning electron microscopy.Karnovsky MJ, Unanue ER.Lab Invest. 1978 Dec;39(6):554-64.A simple method has been devised for correlating fluorescence microscopy and scanning electron microscopy, whereby the identical cell observed by the former can be observed by the latter. With this methodology, we have studied the sequence of cell surface changes which occur when mouse B lymphocytes, bearing immunoglobulin (Ig) on their surfaces, interact with fluoresceinated anti-Ig antibodies. Initially, the pattern of staining is diffuse; then, rapidly, patching occurs, followed by the sweeping of the patches into a cap. Accompanying these events are: the disappearance of microvilli, the formation of ruffles and lamellipodia at the pole of the cell opposite the capped pole, the presence of a constriction ring beneath the cap, ameboid shapes, and the translocation of the cell in a direction opposite the cap. Whereas the cell body is smooth, the capped pole frequently shows the presence of numerous microvilli. However, not all capping cells show the changes, and cells capped in the presence of cytochalasin B usually show none of them, except that their surfaces are smooth. Furthermore, T-cells undergoing translocation show the same cell surface changes as do B-cells. Whereas the contractile apparatus of the cell is considered to underlie all these phenomena, it is concluded that the cell surface changes do not result from patching and capping per se, but rather are an expression of the cell translocation also induced by the anti-Ig antibody.