BookChristopher P. Toseland, Natalia Fili, editors.
Summary: This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments.
Contents:
Fluorescence and Labelling: How to choose and what to do
Fluorescent biosensors: design and application to motor proteins
Rapid Reaction Kinetic Techniques
Fluorescence to study the ATPase mechanism of Motor Proteins
Use of pyrene labelled actin to probe actin myosin interactions; kinetic and equilibrium studies
Fluorescent methods to study transcription initiation and transition into elongation
Single-molecule and single-particle imaging of molecular motors in vitro and in vivo
Fluorescence methods in the investigation of the DEAD-box helicase mechanism
Use of Fluorescent Techniques to Study the In Vitro Movement of Myosins
Fluorescence Tracking of Motor proteins in vitro
Measuring Transport of Motor Cargos
Measuring two at the same time: Combining Magnetic Tweezers with Single-Molecule FRET
Using fluorescence to study actomyosin in yeasts.