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  • Book
    Mary Seabrook.
    Contents:
    Creating an effective learning environment
    Teaching in clinical contexts
    Workplace-based assessment and feedback
    Common problems in clinical teaching
    Next steps.
    Digital Access Wiley 2014
  • Article
    Gillis S, Baker PE, Ruscetti FW, Smith KA.
    J Exp Med. 1978 Oct 01;148(4):1093-8.
    Long-term cultures of human cytotoxic T-cell lines (H-CTLL) were established. H-CTLL cells were strictly dependent on growth upon a T-cell growth factor (TCGF) produced by phytohemagglutinin-stimulated normal human peripheral blood lymphocytes. H-CTLL cells were maintained in TCGF-dependent exponential proliferative culture for over 4 mo during which time they continued to mediate stimulator antigen-specific cytotoxicity as measured by a 4-h 51Cr-release assay. H-CTLL cells recovered from cryopreserved stocks and re-established in long-term culture demonstrated similar high levels of antigen-specific cytotoxicity. H-CTLL cells were 95--100% E-rosette positive and expressed normal T and Ia-like cell surface markers. The ability to sustain differentiated antigen-specific T-effector cells in long-term culture may provide a new means for the study of both the mechanism and regulation of T-cell-mediated immunity.
    Digital Access Access Options