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  • Book
    edited by Douglas B. Kirsch.
    Summary: A new addition to the Neurology in Practice series, Sleep Medicine in Neurology is a practical guide for neurologists diagnosing and managing sleep disorders in their patients. As a part of the series, various feature boxes are highlighted throughout. ""Tips and Tricks"" give suggestions on how to improve outcomes through practical technique or patient questioning. In addition, ""Caution"" warning boxes supply helpful advice on how to avoid problems and 'Science Revisited' boxes offer quick reminders of the basic science principles necessary for understanding the presented concepts.

    Contents:
    Introduction to sleep medicine / Douglas B. Kirsch
    The history and physical examination of the sleep patient / Sheila C. Tsai
    Subjective and objective sleep testing / Martha E. Billings and Nathaniel F. Watson
    Using medications to treat insomnia / Andreea Andrei
    Approach to a patient with excessive daytime sleepiness / Rajdeep Singh and Aatif M. Husain
    Approach to a patient with narcolepsy / Ravichand Madala and Rodney A. Radtke
    Evaluation and medical management of sleep disordered breathing / Mihaela H. Bazalakova and Lawrence J. Epstein
    Surgical management of sleep disordered breathing / Masayoshi Takashima, Jaime Gateno, Melinda Davis-Malessevich and Robert Busch
    Medical comorbidities associated with obstructive sleep apnea / Emerson M. Wickwire and Scott G. Williams
    Non-pharmacological treatments of insomnia and circadian rhythm disorder : special focus on neurology patients / Mary Rose
    Parasomnias : diagnosis, evaluation, and treatment / Douglas B. Kirsch
    Restless leg syndrome, periodic limb movements and other movement disorders in sleep / Raman Malhotra
    Sleep and neurological disorders / Maryann C. Deak
    Cognition, driving and sleep / Makoto Kawai
    Pediatric sleep medicine / Shalini Paruthi.
    Digital Access Wiley 2014
  • Article
    Chinchar GD, Sinclair JH.
    J Cell Physiol. 1978 Sep;96(3):333-42.
    Nutritional requirements of amphibian cells in culture were studied for the purpose of modifying a minimal medium in which frog cells could proliferate and which could be used for obtaining drug-resistant and auxotrophic variants. The serum, purine, CO2, and amino acid requirements for ICR 2A (a Rana pipiens haploid cell strain) have been investigated employing two different media: L-15, a nonbicarbonate, amino acid-buffered medium and Eagle's MEM, a bicarbonate-buffered medium. In this paper we present evidence to support the following conclusions: (1) With L-15 as the base medium, 10% fetal calf serum (FCS) supports optimal cell growth during exponential phase. Calf serum, whole, dialyzed, or heat-inactivated, cannot substitute for FCS and, in fact, is inhibitory. (2) Purines are required by ICR 2A cells only if grown in a nonbicarbonate-buffered medium, since the cells under these conditions cannot produce enough endogenous CO2 to support de novo purine synthesis. (3) In addition to the amino acids considered essential for mammalian cells in culture, ICR 2A cells depend upon exogenous asparagine. Glutamine and/or aspartic acid cannot replace the asparagine requirement. However, ICR 2A cells do utilized exogenous glutamine as an oxidative substrate.
    Digital Access Access Options