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  • Book
    Vijai Kumar Gupta, Maria G. Tuohy, editors ; Manimaran Ayyachamy, Kevin M. Turner, Anthonia O'Donovan, associate editors.
    Summary: Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals.
    Digital Access Springer 2013
  • Article
    Kipps TJ, Benacerraf B, Dorf ME.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2914-7.
    The anti-hapten responses to the dinitrophenyl (Dnp) conjugate of the random linear terpolymers poly(L-Glu,LLys,LPhe), GLphi, or poly(LGlu,LLys,LAla), GLA, are of highly restricted heterogeneity. Thus, individual mice of most responder strains express an average of two to four anti-Dnp clones to Dnp-GLphi or Dnp-GLA, as measured by isoelectric focusing techniques. To explore whether suppressor T cells regulate the heterogeneity of the anti-hapten response to Dnp conjugates of these polypeptides, various procedures aimed at reducing suppressor T-cell activity were tested for their ability to alter the restricted isoelectric focusing spectrotypes. These procedures, such as adult thymectomy, administration of low doses of cyclophosphamide, and in vivo treatment with anti-I-J antisera, significantly increased the magnitude and heterogeneity of the anti-Dnp antibody response to Dnp-GLphi and Dnp-GLA in strains of mice that possessed responder Ir genes. Such methods failed to alter the nonresponder status of mice that lacked the appropriate Ir genes. Thus, in systems under Ir gene control suppressor T-cell mechanisms appear to be involved in the regulation of the heterogeneity of hapten-specific B-cell responses.
    Digital Access Access Options