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- ArticleOhrloff C, Stoffel C, Koch HR, Wefers U, Bours J, Hockwin O.Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1978 Jan 31;205(2):73-9.A tryptophan-free diet induces posterior subcapsular cataracts and reversible corneal opacities in young Wistar rats. Compared to the controls there is a significant decrease of body weight, lens fresh weight, and water-soluble lens protein. Protein separation by isoelectric focusing shows diminished alpha-, beta-, and gamma-crystallin fractions. If tryptophan is restored to the diet after 24 days, new clear lens fibers are laid down again, indicating that the mechanism of protein synthesis has not been permanently damaged by tryptophan-deficiency.