Bookedited by Joel H. Rothman, Andrew Singson.
Summary: An updated edition of the classic Methods in Cell Biology volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.
Contents:
Pt.I Imaging instrumentation : High-resolution imaging of cellular processes in caenorhabditis elegans / Amy S. Maddox, Paul S. Maddox
Immunofluorescence microscopy / Diane C. Shakes, David M. Miller III, Michael L. Nonet
Fluorescent protein methods : strategies and applications / Harald Hutter
Modern electron microscopy methods for C. elegans / David H. Hall, Erika Hartwieg, Ken C.Q. Nguyen
Pt.II Cell biology : Culture and manipulation of embryonic cells / Lois G. Edgar, Bob Goldstein
Laser microsurgery in caenorhabditis elgans / Christopher Fang-Yen, Christopher V. Gabel, Aravinthan D.T. Samuel, Cornelia I. Bargmann, Leon Avery
Methods in cell biology : analysis of cell polarity in C. elegans embryos / Olaf Bossinger, Carrie R. Cowan
Analysis of membrane-bound organelles / Hanna Fares, Alexander M. van der Bliek
C. elegans cell cycle analysis / Sander van den Heuvel, Edward T. Kipreos
Pt.III Physiology : Methods for studying programmed cell death in C. elegans / Yi-Chun Wu, Xiaochen Wang, Ding Xue
Methods for studying the DNA damage response in the caenorhabdatis elegans germ line / Ashley L. Craig, Sandra C. Moser, Aymeric P. Bailly, Anton Gartner
Analysis of aging in caenorhabditis elegans / Deepti S. Wilkinson, Rebecca C. Taylor, Andrew Dillin
Analyses of C. elegans fat metabolic pathways / Alexandre Guimarães de Almeida Barros, Jason Liu, George A. Lemieux, Brendna C. Mullaney, Kaveh Ashrafi
Electrophysiological methods for caenorhabditis elegans neurobiology / Miriam B. Goodman, Theodore H. Lindsay, Shawn R. Lockery, Janet E. Richmond.