BookJonathan D. Dinman, editor.
Summary: When quantum mechanics was first proposed a century ago, nobody could have anticipated how deeply it would affect our lives. Today, we are connected and powered through devices whose existence is predicated on the basic principles of this strange physics. Not even the biological sciences have escaped its reach. As scientists query the deepest mysteries of the living world, the physical scales probed and the types of questions asked are increasingly blurring the lines between biology and physics. The hybrid field of biophysics represents the new frontier of the 21st century. The ribosome has been at the heart of three Nobel Prizes. Understanding its essential nature and how it interacts with other proteins and nucleic acids to control protein synthesis has been one of the central foundations in our understanding of the biology at the molecular level. With the advent of atomic scale structures, methods to visualize and separate individual molecules, and the computational power to model the complex interactions of over a million atoms at once, our understanding of how gene expression is controlled at the level of protein translation is now deeply ensconced in the biophysical realm. This book provides a premier resource to a wide audience, whether it be the general reader seeking a broad view of the field, a clinician interested in the role of protein translation in human disease, the bench researcher looking for state-of-the-art technologies, or computational scientists involved in cutting edge molecular modeling.
Contents:
X-Ray Analysis of Prokaryotic and Eukaryotic Ribosomes / Lasse B. Jenner, Adam Ben-Shem, Natalia Demeshkina, Marat Yusupov and Gulnara Yusupova
A Passage Through the Ribosome by Cryo-EM / Partha P. Datta and Ananya Chatterjee
Molecular Dynamics Simulations of the Ribosome / Karissa Y. Sanbonmatsu, Scott C. Blanchard and Paul C. Whitford
Structural Analyses of the Ribosome by Chemical Modification Methods / Jonathan A. Leshin, Arturas Meskauskas and Jonathan D. Dinman
Methods for Studying the Interactions of Translation Factors with the Ribosome / Assen Marintchev
Riboproteomic Approaches to Understanding IRES Elements / Encarnacion Martinez-Salas, David Piñeiro and Noemi Fernandez
Rapid Kinetic Analysis of Protein Synthesis / Marina V. Rodnina and Wolfgang Wintermeyer
Investigating RNAs Involved in Translational Control by NMR and SAXS / Kathryn D. Mouzakis, Jordan E. Burke and Samuel E. Butcher
Analyses of RNA-Ligand Interactions by Fluorescence Anisotropy / Aparna Kishor, Gary Brewer and Gerald M. Wilson
Approaches for the Identification and Characterization of RNA-Protein Interactions / Saiprasad Palusa and Jeffrey Wilusz
A Multidisciplinary Approach to RNA Localisation / Russell S. Hamilton, Graeme Ball and Ilan Davis
Virtual Screening for RNA-Interacting Small Molecules / Hyun-Ju Park and So-Jung Park
The "Fifth" RNA Nucleotide: A Role for Ribosomal RNA Pseudouridylation in Control of Gene Expression at the Translational Level / Mary McMahon, Cristian Bellodi and Davide Ruggero
Translational Control of Synaptic Plasticity and Memory / Arkady Khoutorsky, Christos Gkogkas and Nahum Sonenberg.