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  • Book
    Michael A. Rapoff.
    Contents:
    Definitions of adherence, types of adherence, problems, and adherence rates
    Consequences of nonadherence and correlates of adherence
    Adherence theories: review, critique, and clinical implications
    Measurement issues: assessing adherence and disease and health outcomes
    Strategies for improving adherence to pediatric medical regimens
    Review of adherence intervention studies and top ten ways to advance research on adherence to pediatric medical regimens.
    Digital Access Springer 2010
  • Article
    McKeehan WL, McKeehan KA, Hammond SL, Ham RG.
    In Vitro. 1977 Jul;13(7):399-416.
    A new medium (MCDB 104) has been developed which will support clonal growth of WI-38 cells at concentrations of serum protein as low as 25 micrograms per ml (equivalent to 0.05% serum). The principal factors responsible for reduction of the protein requirement are: (a) adjustment of all nutrient concentrations in medium F12 to experimentally determined optimum values for WI-38 cells; (b) supplementation with trace elements; (c) replacement of hypoxanthine and folic acid with adenine and folinic acid; and (d) coating of the culture surface with polylysine. Individually, many of these modifications exert only a small effect on cellular growth at reduced protein concentrations, but collectively their effect has been very substantial. Other strains of fibroblast-like human diploid cells from amniotic fluid, fetal lung and newborn foreskin also will grow at reduced concentrations of serum protein in the new medium.
    Digital Access Access Options