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  • Book
    John D. Bisognano, G. Ronald Beck, Ryan W. Connell (editors).
    Summary: An ever growing population of patients are affected by cardiac disease. Most of these diseases are chronic in nature and require long term outpatient follow up. Subsequently, outpatient providers are finding themselves responsible for an increasing number of patients with cardiovascular issues. Appropriate diagnosis and management of cardiac disease is essential to providing high quality care in an efficient manner. There is a wide spectrum of providers involved in the care of these patients, many of which are not cardiologists. The Manual of Outpatient Cardiology is written in a practical, didactic format designed to deliver point-of-care information to practitioners of cardiology and assist non-cardiologists with the efficient management of cardiac disease. Highly illustrated with schematics and useful clinical figures, this book is an essential reference to all outpatient cardiology procedures.

    Contents:
    Part I. Cardiac Diagnostics
    1. History and physical
    2. Evaluation of heart rhythms
    3. Cardiac imaging
    4. Cardiac stress testing
    5. Coronary artery evaluation
    Section II. Common Cardiac Diseases
    6. Hypertension
    7. Dyslipidemia
    8. Coronary artery disease
    9. Valvular heart disease
    10. Ambulatory arrhythmias
    11. Congestive heart failure
    12. Vascular diseases, peripheral/aorta
    Section III. Approach to the Patient
    13. Approach to the patient with chest pain
    14. Syncope, work up and management
    15. Palpitations
    16. New murmur
    17. Approach to the patient with dyspnea
    18. Pre-operative cardiovascular risk assessment for non-cardiac surgery
    19. Approach to the patient with cardiovascular disease and activity limitations
    Index.
    Digital Access Springer 2012
  • Article
    Pifer LL, Hughes WT, Murphy MJ.
    Pediatr Res. 1977 Apr;11(4):305-16.
    Pneumocystis carinii was propagated in vitro with primary embryonic chick epithelial lung (CEL) cells. Viability and growth of the organism were demonstrated by direct observation of the reproductive cycle in the Sykes-Moore chamber, serial passage with an increase in the number of mature cysts forms, the cytopathic effect of the organism on cell culture, and inhibition of growth of the organism by specific antiserum and pentamidine isethionate. Attempts to culture P. carinii indefinitely were not successful. However, cyst forms derived from murine and human sources increased 100-fold and 10-fold, respectively, during CEL cell culture passages. Serial passage of trophozoites alone resulted in the development of typical CPE and a maximum number of 2.8 X 10(3) cyst forms. Autoradiographic methods demonstrated active DNA, RNA, and protein synthesis within the cyst and suggest that metabolic interaction between the host cells and the organisms occurred. The nature of the attachment of P. carinii to the host CEL cell was clearly discernible by scanning electron microscopy (SEM). In the reproductive cycle a vegetative cell (designated "trophozoite") attached by tubular expansions to the hhed without entering the cell. Sporozoites developed within the detached young cyst, reaching a maximum number of eight within the mature cyst. Excystment occurred through single or multiple sites in the cyst wall, after which the released trophozoite attached to a new host cell.
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