Bookedited by Mohamed Al-Rubeai.
Summary: Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This book deals with the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity and improve cell function.
Contents:
Use of MAR Elements to Increase the Production of Recombinant Proteins
Expression Engineering: The IE2 Promoter/Enhancer from Mouse CMV
Defeating Randomness: Targeted Integration as a Boost for Biotechnology
Importance of Genetic Environment for Recombinant Gene Expression
Expression Vector Engineering for Recombinant Protein Production
Cell Xpress Applications in Development and Characterization of Biopharmaceutical Recombinant Protein Producing Cell Lines
Selection Methods for High-Producing Mammalian Cell Lines
Engineering Mammalian Cells for Recombinant Monoclonal Antibody Production
Engineering Cell Function by RNA Interference
Apoptosis and Autophagy Cell Engineering
Glycoengineering and Modeling of Protein N-Glycosylation
Engineering the Secretory Pathway in Mammalian Cells.