Today's Hours: 12:00pm - 8:00pm
Lane Medical Library

Search

Filter Results

Did You Mean:

Search Results

Sort by
relevance
  • Journal
    Digital Access HathiTrust v. 1-93, 1830-1922.
    Print Access
    Location
    Version
    Call Number
    Items
    Stored offsite. Please request print.
    v. 21-94, 1850-1923.
    75
    Request
    Medical History Center: By appointment only.
    v. 1-20, 1830-1849.
    22
  • Article
    Johnson RG, Scarpa A, Salganicoff L.
    J Biol Chem. 1978 Oct 10;253(19):7061-8.
    The [14C]methylamine distribution method was utilized to measure the internal pH of isolated serotonin containing granules of pig blood platelets under varying conditions. The granules used were isolated by a new protocol which stressed platelet rupture under controlled conditions and preservation of isotonicity throughout the isolation procedure, In a well buffered external medium, pH 6.85, The deltapH was measured as 1.11 with the internal pH being found acidic (pH 5.74). Increasing the external pH produced a corresponding increase in the deltaH. The pH gradient could be collapsed by the addition of ionophores and uncouplers which are known to transport protons across biological membranes. In addition, the deltapH was constant for granules suspended in various ionic media, thus suggesting that the deltaH did not arise secondarily due to the establishment of a Donnan equilibrium. The existence of the acidic intragranular space is discussed with respect to previous ancillary findings. Also, an explication of the possible physiological significance of the deltaH is presented.
    Digital Access Access Options