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- ArticleGantotti BV, Patil SS, Mandel M.Mol Gen Genet. 1979 Jul 02;174(1):101-3.The bacterium Pseudomonas phaseolicola was successfully transformed, for the first time, with R-factors RSF1010 and pBR322 DNA by a calcium-shock and heat-pulse technique. Frequency of transformation for RSF1010 ranged from 0.8 x 10(-7) to 3.1 x 10(-6) and was ca. 0.4 x 10(-8) for pBR322.