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- JournalDigital Access TandFonline v. 9-16, 1965-71.Access via Acta rheumatologica Scandinavica 1955-1971LocationVersionCall NumberItems
- ArticleMertvetsov NP, Il'duganova NA, Chesnokov VN, Blinova NN.Biokhimiia. 1978 Jun;43(6):959-64.In vitro estimation of synthesis of inducible tyrosine aminotransferase isoenzyme, directed by poly-A-containing RNA from liver of intact and corticol treated rats, is carried out. Total poly-A-containing RNA from liver polyribosomes of intact and induced rats was translated in cell-free system from wheat germs. Two antibodies immunoprecipitation was used to identify the translocation product (tyrosine aminotransferase). It was found that a synthesis of a specific protein product, precipitated by antibodies to tyrosine aminotransferase, takes place in cell-free system under translation of polysomic poly-A-containing liver RNA. The amount of immunoprecipitated product indicates, that the content of individual poly-A-containing mRNA for inducible tyrosine aminotransferase isoenzyme in liver of cortisol-induced rats is considerably higher than in intact animals.