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- ArticleFrisch-Niggemeyer W.Acta Virol. 1975 Sep;19(5):381-6.Purified preparations of haemagglutinins from Sindbis, West Nile and tick-borne encephalitis (TBE) viruses could be obtained from infected mouse brains by alkaline extraction, precipitation with protamine and chromatography on polyethylene glycol-coated controlled pore glass with 242 A pore diameter.