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  • Book
    edited by Sylvie Doublié.
    Digital : Springerv. 1, 2006
    Digital : Springerv. 2, 2006
    v. 1. A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag / Joseph E. Tropea ... [et al.] -- Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project / Sophie Quevillon-Cheruel ... [et al.] -- Baculoviral Expression of an Integral Membrane Protein for Structural Studies / Dean R. Madden and Markus Safferling -- Protein Engineering / Sonia, Longhi, Francois Ferron, and Marie-Pierre Egloff -- Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems / Sylvie Doublié -- How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals / Gloria E. O. Borgstahl -- Screening and Optimization Methods for Nonautomated Crystallization Laboratories / Terese Bergfors -- Improving Marginal Crystals / Charles W. Carter, Jr. and Madeleine Riès-Kautt -- Optimization Techniques for Automation and High Throughput / Naomi E. Chayen -- Three-Dimensional Crystallization of Membrane Proteins / James Féthière -- Crystallization of Protein-DNA Complexes / Thomas Hollis -- Preparation and Crystallization of RNA / Barbara L. Golden -- Crystallization of RNA-Protein Complexes / Eiji Obayashi ... [et al.] -- Index. v. 2. Cryocrystallography of Macromolecules: Practice and Optimization / Elspeth Garman and Robin L. Owen -- Determination of Reaction Intermediate Structures in Heme Proteins / Kelvin Chu -- Annealing Macromolecular Crystals / B. Lief Hanson and Gerard J. Bunick -- First Analysis of Macromolecular Crystals: Biochemistry and X-Ray Diffraction / David Jeruzalmi -- X-Ray Data Collection from Macromolecular Crystals / Elspeth Garman and Robert M. Sweet -- Characterizing a Crystal from an Initial Native Dataset / Michael R. Sawaya -- Molecular Replacement / Eric A. Toth -- Phase Determination using Halide Ions / Miroslawa Dauter and Zbigniew Dauter -- The Same But Different: Isomorphous Methods for Phasing and High-Throughput Ligand Screening / Mark A. Rould -- Substructure Determination in Multiwavelength Anomalous Diffraction, Single Anomalous Diffraction, and Single Isomorphous Replacement with Anomalous Scattering Data Using Shake-and-Bake / G. David Smith, Christopher T. Lemke, and P. Lynne Howell -- Substructure Determination in Isomorphous Replacement and Anomalous Diffraction Experiments / Ralf W. Grosse-Kunstleve and Thomas R. Schneider -- Automated Structure Solution with autoSHARP / Clemens Vonrhein, Eric Blanc, Pietro Roversi, and Gérard Bricogne -- Introduction to Macromolecular Refinement / Dale E. Tronrud -- Quality Control and Validation / Gerard J. Kleywegt -- Crystallographic Software: A Sustainable Resource for the Community / Stephen J. Everse and Sylvie Doublié -- Index. v. 1. Preparation and crystallization of macromolecules -- v. 2. Structure determination.