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- Bookedited by Sylvie Doublié.Contents:
v. 1. A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag / Joseph E. Tropea ... [et al.]
Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project / Sophie Quevillon-Cheruel ... [et al.]
Baculoviral Expression of an Integral Membrane Protein for Structural Studies / Dean R. Madden and Markus Safferling
Protein Engineering / Sonia, Longhi, Francois Ferron, and Marie-Pierre Egloff
Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems / Sylvie Doublié
How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals / Gloria E. O. Borgstahl
Screening and Optimization Methods for Nonautomated Crystallization Laboratories / Terese Bergfors
Improving Marginal Crystals / Charles W. Carter, Jr. and Madeleine Riès-Kautt
Optimization Techniques for Automation and High Throughput / Naomi E. Chayen
Three-Dimensional Crystallization of Membrane Proteins / James Féthière
Crystallization of Protein-DNA Complexes / Thomas Hollis
Preparation and Crystallization of RNA / Barbara L. Golden
Crystallization of RNA-Protein Complexes / Eiji Obayashi ... [et al.]
Index. v. 2. Cryocrystallography of Macromolecules: Practice and Optimization / Elspeth Garman and Robin L. Owen
Determination of Reaction Intermediate Structures in Heme Proteins / Kelvin Chu
Annealing Macromolecular Crystals / B. Lief Hanson and Gerard J. Bunick
First Analysis of Macromolecular Crystals: Biochemistry and X-Ray Diffraction / David Jeruzalmi
X-Ray Data Collection from Macromolecular Crystals / Elspeth Garman and Robert M. Sweet
Characterizing a Crystal from an Initial Native Dataset / Michael R. Sawaya
Molecular Replacement / Eric A. Toth
Phase Determination using Halide Ions / Miroslawa Dauter and Zbigniew Dauter
The Same But Different: Isomorphous Methods for Phasing and High-Throughput Ligand Screening / Mark A. Rould
Substructure Determination in Multiwavelength Anomalous Diffraction, Single Anomalous Diffraction, and Single Isomorphous Replacement with Anomalous Scattering Data Using Shake-and-Bake / G. David Smith, Christopher T. Lemke, and P. Lynne Howell
Substructure Determination in Isomorphous Replacement and Anomalous Diffraction Experiments / Ralf W. Grosse-Kunstleve and Thomas R. Schneider
Automated Structure Solution with autoSHARP / Clemens Vonrhein, Eric Blanc, Pietro Roversi, and Gérard Bricogne
Introduction to Macromolecular Refinement / Dale E. Tronrud
Quality Control and Validation / Gerard J. Kleywegt
Crystallographic Software: A Sustainable Resource for the Community / Stephen J. Everse and Sylvie Doublié
Index. v. 1. Preparation and crystallization of macromolecules
v. 2. Structure determination.Digital Access - ArticleO'Neill IK, Pringuer MA, Prosser HJ.J Pharm Pharmacol. 1975 Apr;27(4):222-5.A method based on nuclear magnetic resonance spectroscopy is described for the quantitative determination of polyimethylsiloxane (PMS) in a variety of pharmaceutical preparations. An extraction procedure using deuterochloroform within the sample tube was devised. In the presence of an internal standard, the PMS concentration was determined directly using integrated peak areas, without the necessity of establishing calibration curves. Accurate quantitative results were achieved with sample, containing at least 2mg PMS, extracted from formulations containing greater than 0.5% PMS. The extraction procedure and nmr assay were both more efficient and convenient than the B.P.C. method.