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- ArticleLi M, Ge A, Liu M, Ma B, Ma C, Shi C.Talanta. 2020 Nov 01;219:121221.Hand-powered centrifugal microfluidics combined with isothermal nucleic acid amplification testing (NAAT) have been one of the most promising rapid detection platforms in resource-limited settings. However, current hand-powered centrifuges still suffer from customized instrument-based operation and low rotation rate; and most isothermal NAAT were conducted with complicated reaction systems for DNA detection and required an additional step for RNA detection. Herein, we built a fully hand-powered centrifugal miniaturized NAAT platform inspired by buzzer toys, which embedded sample preparation, strand exchange amplification (SEA) and visual fluorescence detection together. The centrifugal disc was easily fabricated, and operated the mixing in 1 min by simply dragging the looped rope through it with a mean input force of 16.5 N, enabling its rotation rate reach 5000 rpm. In addition, SEA was an ultra-simple one-step DNA or RNA detection method initiated by Bst DNA polymerase and a pair of primers, and thus we took all its merits and integrate it into microfluidic systems firstly. Furthermore, taking Vibrio parahemolyticus as an example, the microfluidic platform achieved DNA or RNA detection within 1 h; and the detection limit of the microchip for artificially spiked oysters was 103 CFU/g without cumbersome sample preparation, and reached to 100 CFU/g after enrichment. Therefore, we provided an ultra-simple and non-instrumental microfluidic platform powered merely by hands, performing general potential in sample-to-answer NAAT for versatile pathogens in remote regions.
- ArticleOsińska A, Korzeniewska E, Harnisz M, Felis E, Bajkacz S, Jachimowicz P, Niestępski S, Konopka I.J Hazard Mater. 2020 01 05;381:121221.Wastewater treatment plants (WWTPs) are significant source of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs), which can spread further in the environment by reaching rivers together with effluents discharged from WWTPs. In this study untreated and treated wastewater (UWW, TWW), upstream and downstream river water (URW, DRW) were collected from 4 WWTPs, in the winter and autumn seasons. The occurrence of ARB resistant to beta-lactams and tetracyclines as well as the presence of antibiotics from these classes were analysed in water and wastewater samples. Additionally, the amounts of 12 ARGs, 2 genes of mobile genetic elements (MGEs), gene uidA identifying E. coli and 16S rRNA were also determined. Resistance to beta-lactams prevailed among ARB in water and wastewater samples (constituting 82-88% of total counts of bacteria). The dominant genes in water and wastewater samples were blaTEM, tetA, sul1. The gene blaOXA demonstrated high variability of its concentration in samples collected in both seasons. Despite the high per cent reduction of ARB and ARGs concentration observed during the wastewater treatment processes, their large quantities are still transmitted into the environment. The research focuses on WWTPs' role in the dissemination of ARGs and MGEs in the aquatic environment.
- ArticleZhang J, Liu H, Wang J, Huang Q.J Environ Manage. 2024 Jun;360:121221.A thorough study of the spatial impact of the digital economy on low-carbon logistics efficiency would be greatly significant for Regional Comprehensive Economic Partnership (RCEP) countries to improve low-carbon logistics efficiency and achieve sustainable cooperation. This study constructs a theoretical framework from the perspective of spatial effects on the impact of the digital economy on low-carbon logistics efficiency in RCEP countries. The entropy method was used to measure the level of digital economic development. The super-efficiency SBM model was used to measure low-carbon logistics efficiency. Spatial feature analysis was conducted using kernel density estimation and Moran's index, followed by empirical analysis using spatial econometric models to examine the spatial impact of the digital economy on low-carbon logistics efficiency in RCEP countries. The results indicate that in RCEP countries, both low-carbon logistics efficiency and the level of digital economic development exhibit significant spatial positive correlation. Furthermore, the digital economy can promote low-carbon logistics efficiency in economically neighboring countries through spatial spillover effects. The improvement of domestic low-carbon logistics efficiency can also promote low-carbon logistics efficiency in neighboring countries. This conclusion was supported by endogeneity tests and a convergence analysis. Additionally, the mechanism analysis revealed that improving the level of green energy can enhance the spatial spillover effects of the digital economy and promote low-carbon logistics efficiency. Finally, countermeasures and suggestions was proposed to improve the low-carbon logistics efficiency of RCEP countries through the digital economy.
- ArticleAja E, Mishra A, Dou Y, Fletcher HM.Microbiol Spectr. 2021 12 22;9(3):e0121221.In the periodontal pocket, there is a direct correlation between environmental conditions, the dynamic oral microbial flora, and disease. The relative abundance of several newly recognized microbial species in the oral microenvironment has raised questions on their impact on disease development. One such organism, Filifactor alocis, is significant to the pathogenic biofilm structure. Moreover, its pathogenic characteristics are highlighted by its ability to survive in the oxidative-stress microenvironment of the periodontal pocket and alter the microbial community dynamics. There is a gap in our understanding of its mechanism(s) of oxidative stress resistance and impact on pathogenicity. Several proteins, including HMPRFF0389-00519 (FA519), were observed in high abundance in F. alocis during coinfection of epithelial cells with Porphyromonas gingivalis W83. Bioinformatics analysis shows that FA519 contains a "Cys-X-X-Cys zinc ribbon domain" which could be involved in DNA binding and oxidative stress resistance. We have characterized FA519 to elucidate its roles in the oxidative stress resistance and virulence of F. alocis. Compared to the wild-type strain, the F. alocis isogenic gene deletion mutant, FLL1013 (ΔFA519::ermF), showed significantly reduced sensitivity to hydrogen peroxide and nitric oxide-induced stress. The ability to form biofilm and adhere to and invade gingival epithelial cells was also reduced in the isogenic mutant. The recombinant FA519 protein was shown to protect DNA from Fenton-mediated damage with an intrinsic ability to reduce hydrogen peroxide and disulfide bonds. Collectively, these results suggest that FA519 is involved in oxidative stress resistance and can modulate important virulence attributes in F. alocis. IMPORTANCE Filifactor alocis is an emerging member of the periodontal community and is now proposed to be a diagnostic indicator of periodontal disease. However, due to the lack of genetic tools available to study this organism, not much is known about its virulence attributes. The mechanism(s) of oxidative stress resistance in F. alocis is unknown. Therefore, identifying the adaptive mechanisms utilized by F. alocis to survive in the oxidative stress environment of the periodontal pocket would lead to understanding its virulence regulation, which could help develop novel therapeutic treatments to combat the effects of periodontal disease. This study is focused on the characterization of FA519, a hypothetical protein in F. alocis, as a multifunctional protein that plays an important role in the reactive oxygen species-detoxification pathway. Collectively, our results suggest that FA519 is involved in oxidative stress resistance and can modulate important virulence attributes in F. alocis.
- ArticleHong M, Wang J, Yang B, Liu Y, Sun X, Li L, Yu S, Liu S, Kang Y, Wang W, Qiu G.Water Res. 2024 Mar 15;252:121221.This study proposes a novel method by forming biogenic K-jarosite coatings on pyrite surfaces driven by Acidithiobacillus ferrooxidans (A. ferrooxidans) to reduce heavy metal release and prevent acid mine drainage (AMD) production. Different thicknesses of K-jarosite coatings (0.7 to 1.1 μm) were able to form on pyrite surfaces in the presence of A. ferrooxidans, which positively correlated with the initial addition of Fe2+ and K+ concentrations. The inhibiting effect of K-jarosite coatings on pyrite oxidation was studied by electrochemical measurements, chemical oxidation tests, and bio-oxidation tests. The experimental results showed that the best passivation performance was achieved when 20 mM Fe2+ and 6.7 mM K+ were initially introduced with a bacterial concentration of 4 × 108 cells·mL-1, reducing chemical and biological oxidation by 70 % and 98 %, respectively (based on the concentration of total iron dissolved into the solution by pyrite oxidation). Similarly, bio-oxidation tests of two mine waste samples also showed sound inhibition effects, which offers a preliminary demonstration of the potential applicability of this method to actual waste rock. This study presents a new perspective on passivating the oxidation of metal sulfide tailings or waste and preventing AMD.
- ArticleCummins ML, Reid CJ, Djordjevic SP.mSystems. 2022 02 22;7(1):e0121221.Escherichia coli sequence type 95 (ST95) is an extraintestinal pathogenic E. coli (ExPEC) renowned for its ability to cause significant morbidity and mortality in humans and poultry. A core genome analysis of 668 ST95 isolates generated 10 clades (A to J), 5 of which are reported here for the first time. F plasmid replicon sequence typing showed that almost a third (178/668 [27%]) of the collection carry pUTI89 (F29:B10) and were restricted to clade A and a sublineage of clade B. In contrast, almost half (328/668 [49%]) of the collection across multiple clades harbor ColV plasmids (multiple F types). Strikingly, ST95 lineages with pUTI89 were almost exclusively from humans, while ColV+ ST95 lineages were sourced from poultry and humans. Clade I was notable because it comprises temporally and geographically matched ColV+ isolates sourced from human and retail poultry meat, suggesting interspecies transmission via food. Clade F contained ST95 isolates of bovine origin, none of which carried ColV or pUTI89 plasmids. Remarkably, an analysis of a cohort of 34,176 E. coli isolates comprising 2,570 sequence types mirrored what was observed in ST95: (i) pUTI89 was overwhelmingly linked to E. coli sourced from humans but almost entirely absent from 13,027 E. coli isolates recovered from poultry, pigs, and cattle, and (ii) E. coli isolates harboring ColV plasmids were from multiple sources, including humans, poultry, and swine. Overall, our data suggest that F plasmids influence E. coli host range, clade structure, and zoonotic potential in ST95 and ExPEC more broadly. IMPORTANCE E. coli ST95 is one of five dominant ExPEC lineages globally and noted for causing urinary tract and bloodstream infections and neonatal meningitis in humans and colibacillosis in poultry. Using high-resolution phylogenomics, we show that F replicon sequence type is linked to ST95 clade structure and zoonotic potential. Specifically, human centric ST95 clades overwhelmingly harbor F29:B10 (pUTI89) plasmids, while clades carrying both human- and poultry-sourced isolates are typically ColV+ with multiple replicon types. Importantly, several clades identified clonal ColV+ ST95 isolates from human and poultry sources, but clade I, which housed temporally and spatially matched isolates, provided the most robust evidence. Notably, patterns of association of F replicon types with E. coli host were mirrored within a diverse collection of 34,176 E. coli genomes. Our studies indicate that the role of food animals as a source of human ExPEC disease is complex and warrants further investigation.
- ArticleKumar S, Yadav Ravulapalli S, Kumar Tiwari S, Gupta S, Nair AB, Jacob S.Int J Pharm. 2021 Dec 15;610:121221.The cassette dosing technique is employed in the drug discovery stage of non-clinical studies to obtain pharmacokinetic data from multiple drug candidates in a single experiment. The objective of the current investigation was to evaluate the effect of sex and food on the selected pharmacokinetic parameters of four biopharmaceutical classification system (BCS) drugs (BCS-I: propranolol, BCS-II: diclofenac, BCS-III: atenolol, and BCS-IV: acetazolamide) utilizing cassette dosing in male and female rats under fed and fasting conditions. Different animal groups were dosed intravenous (i.v) and oral at 1 and 10 mg/kg, respectively, in the form of cassette at a dose of 5 mL/kg. Blood samples were analyzed by liquid chromatography-tandem mass spectrometry. Pharmacokinetics parameters were calculated using Phoenix software version 8.1. A significant increase (p < 0.05) of the area under the plasma concentration-time (AUC0-last) was observed for diclofenac and acetazolamide in females over males after i.v dosing. Additionally, acetazolamide showed greater instantaneous concentration at the time of dosing, and clearance in females (p < 0.05) compared to males after i.v administration. After oral dosing, propranolol exhibited significant variations (p < 0.05) in the maximum drug concentration (Cmax), AUC0-last, the volume of distribution (Vd), and bioavailability in females as compared to males under fed state. Diclofenac showed significant changes (p < 0.05) in AUC0-last, and clearance (Cl) in females as compared to males under fasting and fed state. However, acetazolamide exhibited a significant enhancement (p < 0.05) in AUC0-last, Vd, and Cl in fasting females than the males. The data here illustrates that there is an appreciable difference in AUC and Cmax values exist in male and female rats under fed and fasting conditions administered with the cassette dosing of tested BCS class drugs.
- ArticleCarr EL, Wilson ME, Adams ST, Arens DK, Ayala M, Ayers H, Barker A, Beecroft V, Bishop E, ... Show More Brundage B, Carroll MJ, Chow J, Cobbley H, Davis R, Fajardo C, Flor S, Fuhriman D, Tullis RG, Gleave A, Green C, Hanis T, Hoggan T, Johnson L, Kruger JL, Lambert A, Lazaro EC, Loertscher E, Marshall N, Melhado E, Sarabia R, Sharma R, Steffensen A, Stewart JB, Stoker T, Swain A, Toronto S, Thompson DW, Todd JZ, Walker J, Wilkey A, Wilson D, Hallen CL, Casjens SR, Grose JH.Microbiol Resour Announc. 2022 May 19;11(5):e0121221.We announce the complete genome sequences of 14 Serratia bacteriophages isolated from wastewater treatment plants. These phages define two previously undescribed types which we call the Carrot-like phage cluster (phages Carrot, BigDog, LittleDog, Niamh, Opt-148, Opt-169, PhooPhighters, Rovert, Serratianator, Stoker, Swain, and Ulliraptor) and Tlacuache-like phage cluster (Tlacuache and Opt-155).
- ArticleMa S, Liu W, Meng C, Dong J, Zhang S.Environ Pollut. 2023 Apr 15;323:121221.Particulate matter emitted by heated cooking oil is hazardous to human health. To develop effective mitigation strategies, it is critical to know the amount of the emitted particles. The purpose of this research is to estimate the temperature-dependent particle mass emission rates of edible oils and to develop models for source strength based on the multiple linear regression method. First, this study examined seven commonly used oils by heating experiments. The emission rates of PM2.5 and PM10 were measured, and the effects of parameters such as oil volume and surface area on the emission rates were also analysed. Following that, the starting smoke points (Ts') and aggravating smoke points (Tss') of tested oils were determined. The results showed that oils with lower smoke points had greater emission rates. Notably, the experiments performed observed that peanut, rice, rapeseed and olive oil generated PM2.5 much faster at 240 °C (2.22, 1.50, 0.82 and 0.80 mg/s, respectively, at the highest emission conditions) than that of sunflower, soybean, and corn oil (0.15, 0.12 and 0.11 mg/s, respectively). The temperature, volume, and surface area of oils all had a significant impact on the particle mass emission rate, with oil temperature being the most influential. The regression models obtained were statistically significant (P < 0.001), with the majority of R2 values greater than 0.85. Using sunflower, soybean and corn oils, which have higher smoke points and lower emission rates, and smaller pans for cooking is therefore recommended based on our research findings.
- ArticleHenriksson PJ, Heijungs R, Dao HM, Phan LT, de Snoo GR, Guinée JB.PLoS One. 2015;10(3):e0121221.In response to growing awareness of climate change, requests to establish product carbon footprints have been increasing. Product carbon footprints are life cycle assessments restricted to just one impact category, global warming. Product carbon footprint studies generate life cycle inventory results, listing the environmental emissions of greenhouse gases from a product's lifecycle, and characterize these by their global warming potentials, producing product carbon footprints that are commonly communicated as point values. In the present research we show that the uncertainties surrounding these point values necessitate more sophisticated ways of communicating product carbon footprints, using different sizes of catfish (Pangasius spp.) farms in Vietnam as a case study. As most product carbon footprint studies only have a comparative meaning, we used dependent sampling to produce relative results in order to increase the power for identifying environmentally superior products. We therefore argue that product carbon footprints, supported by quantitative uncertainty estimates, should be used to test hypotheses, rather than to provide point value estimates or plain confidence intervals of products' environmental performance.
- ArticleNoda I.Spectrochim Acta A Mol Biomol Spectrosc. 2022 Aug 05;276:121221.A procedure is described to estimate the pure component spectra of mixtures from only a pair of available spectra even when there are more than two component species present in the system. In contrast, traditional multivariate curve resolution (MCR) technique cannot be used for such a case. The method relies on the use of two-trace two-dimensional (2T2D) correlation spectroscopy. Asynchronous 2T2D spectrum is used to identify the characteristic bands most strongly associated with the individual mixture component species. Correlation coefficients derived from the synchronous 2T2D spectrum are used to obtain a set of correlative filtering functions to distribute the spectral intensity of the average spectrum among the estimates of the pure component spectra. Efficacy of the method was demonstrated using a pair of ATR IR spectra obtained for two solution mixtures containing three main ingredients with very similar compositions. Relatively congested and overlapped spectral region was used first for the demonstration, and reasonable resolution was accomplished yielding a set of the estimates of pure component spectra with most of the expected pertinent features included. The analysis was then extended to a broader spectral region containing well-isolated spectral signatures of individual components for positive validation. While traditional MCR technique seems to perform better with a large number of spectra, this technique can be effectively used in conjunction with MCR to improve its stability and performance, especially under some challenging conditions.
- BookCarissa Carter, Scott Doorley ; illustrations by Armando Veve.Summary: "A powerful, fully illustrated guide to using the tools of design thinking to create a more positive and cooperative future, from Stanford University's Hasso Plattner Institute of Design"-- Provided by publisher.PrintLocationVersionCall NumberItems