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- ArticleCoccaro EF, Irwin M, Arevalo JMG, Dizon T, Cole S.Psychoneuroendocrinology. 2022 02;136:105453.Evidence of chronic, systemic, low levels of inflammation is present in several stress-related psychiatric conditions including schizophrenia, depression, and intermittent explosive disorder (IED). We analyzed leukocyte gene expression (mRNA) to quantify the activity of pro and anti-inflammatory signaling pathways. Work performed in non-aggressive populations has uncovered a Conserved Transcriptional Response to Adversity (CTRA) characterized by an upregulation of pro-inflammatory gene transcription in chronically stressed individuals. We used pathway-based bioinformatic analyses of genome-wide transcriptional profiles of peripheral blood leukocyte samples from IED study participants (N = 45) and controls [healthy (n = 45) and psychiatric (n = 34)], with analyses focusing on the pro-inflammatory transcription control pathway mediated by the NF-kB family of transcription factors (typically upregulated in CTRA) and the antiviral transcription control pathway mediated by anti-viral response (IRF) family transcription factors (typically downregulated in CTRA). Compared with both healthy and psychiatric controls, individuals with IED had upregulated transcriptional activity of the antiviral response (IRF), but no evidence of pro-inflammatory NF-kB activation. Analyses implicated CD4 + T cells, CD8 + T cells, and B lymphocytes in IED-related transcriptional alterations, but showed no significant indication of monocyte involvement. This suggests that the inflammatory profile of IED differs substantially from that observed previously in other stress-related disorders, and may involve a pathogen-driven adaptive immune etiology.
- ArticleKouketsu A, Miyashita H, Kojima I, Sakamoto M, Murata T, Mori S, Nogami S, Yamauchi K, Nagai H, Kumamoto H, Takahashi T.Oral Oncol. 2021 09;120:105453.OBJECTIVES: To evaluate the ability of different imaging modalities to accurately detect bone invasion in oral squamous cell carcinomas.
PATIENTS AND METHODS: Patients with oral squamous cell carcinoma, who were scheduled for mandibulectomy or maxillectomy, underwent clinical evaluation using five preoperative imaging diagnosis methods-contrast-enhanced MRI, CT, 99mTc scintigraphy (Tc scan), FDG-PET CT (PET/CT), and panoramic radiography. The sensitivity and specificity of each modality in detecting bone invasion were calculated by comparing the findings on the images with postoperative histopathological findings. In a subgroup of patients, we further assessed the ability of MRI and CT to detect the accurate extent of bone invasion, including the height, width, and depth in patients with pathological mandibular invasion.
RESULTS: Overall, 50 patients were enrolled in this study, and nine patients with pathological mandibular invasion were included in our subgroup analysis. MRI was found to be the most useful method in detecting bone invasion, showing the highest sensitivity (88.9%) and negative predictive values (92.3%). CT (87.5% specificity and 77.8% sensitivity) was more specific than MRI, though less sensitive. Combined PET/CT was more sensitive (83.3%) and less specific (71.9%) than CT. Tc scan had high sensitivity (88.9%); however, the specificity was relatively low (71.9%).
CONCLUSION: MRI was the most useful method in detecting bone invasion. A negative MRI result definitively excludes bone marrow invasion. In patients with positive MRI findings, a negative CT may be useful in ruling out bone marrow invasion. - ArticleAbel M, Bäuml KT.Cognition. 2023 09;238:105453.Social interactions can shape our memories. Here, we examined two well-established effects of collaborative remembering on individual memory: collaborative facilitation for initially studied and social contagion with initially unstudied information. Participants were tested in groups of three. After an individual study phase, they completed a first interpolated test either alone or collaboratively with the other group members. Our goal was to explore how prior collaboration affected memory performance on a final critical test, which was taken individually by all participants. Experiments 1a and 1b used additive information as study materials, whereas Experiment 2 introduced contradictory information. All experiments provided evidence of collaborative facilitation and social contagion on the final critical test, which affected individual memory simultaneously. In addition, we also examined memory at the group level on this final critical test, by analyzing the overlap in identical remembered contents across group members. Here, the experiments showed that both collaborative facilitation for studied information and social contagion with unstudied information contributed to the development of shared memories across group members. The presence of contradictory information reduced rates of mnemonic overlap, confirming that changes in individual remembering have repercussions for the development of shared memories at the group level. We discuss what cognitive mechanisms may mediate the effects of social interactions on individual remembering and how they may serve social information transmission and the formation of socially shared memories.
- ArticleZhao X, Zhang G, Zhang Z.Environ Int. 2020 03;136:105453.Nowadays, an increasing discharge of oxyanions to the natural environment has been attracting worldwide attention. TiO2-based photocatalysis is regarded as one of the most promising technologies for the conversion of toxic oxyanions (such as chromate, nitrate, nitrite, bromate, perchlorate and selenate) to harmless and/or less toxic substances in contaminated waters. Various types of TiO2-based catalysts have been developed, and each of them exhibits its own advantages in catalytic reduction of oxyanions. However, the application of these nanostructured TiO2 in real water bodies remains a challenge, with limitations associated with sunlight harvesting abilities, production costs, reuse stability and exposure risks. Herein, we aim to present a critical review on reported TiO2-based photocatalytic reduction of aqueous oxyanions, provide a comprehensive understanding of the possible reaction pathways of formed active species, and evaluate the reduction performance of different types of TiO2-based catalysts. In addition, the impact of operating parameters (such as solution pH, temperature, dissolved oxygen and coexisting substances) on catalytic reduction performance is discussed. Furthermore, the perspectives of TiO2-based photocatalytic reduction of oxyanions are also proposed.
- ArticleSmit JAH, Vooijs R, Lindenburg P, Baugh AT, Halfwerk W.Horm Behav. 2024 01;157:105453.Urban areas are characterised by the presence of sensory pollutants, such as anthropogenic noise and artificial light at night (ALAN). Animals can quickly adapt to novel environmental conditions by adjusting their behaviour, which is proximately regulated by endocrine systems. While endocrine responses to sensory pollution have been widely reported, this has not often been linked to changes in behaviour, hampering the understanding of adaptiveness of endocrine responses. Our aim was, therefore, to investigate the effects of urbanisation, specifically urban noise and light pollution, on hormone levels in male urban and forest túngara frogs (Engystomops pustulosus), a species with reported population divergence in behaviour in response to urbanisation. We quantified testosterone and corticosterone release rates in the field and in the lab before and after exposure to urban noise and/or light. We show that urban and forest frogs differ in their endocrine phenotypes under field as well as lab conditions. Moreover, in urban frogs exposure to urban noise and light led, respectively, to an increase in testosterone and decrease in corticosterone, whereas in forest frogs sensory pollutants did not elicit any endocrine response. Our results show that urbanisation, specifically noise and light pollution, can modulate hormone levels in urban and forest populations differentially. The observed endocrine responses are consistent with the observed behavioural changes in urban frogs, providing a proximate explanation for the presumably adaptive behavioural changes in response to urbanisation.
- ArticleGuo H, Yi J, Wang F, Lei T, Du H.Neurochem Int. 2023 01;162:105453.Parkinson's disease (PD) is a common chronic neurodegenerative disease, and the heat shock proteins (HSPs) are proved to be of great value for PD. In addition, HSPs can maintain protein homeostasis, degrade and inhibit protein aggregation by properly folding and activating intracellular proteins in PD. This study mainly summarizes the important roles of HSPs in PD and explores their feasibility as targets. We introduced the structural and functional characteristics of HSPs and the physiological functions of HSPs in PD. HSPs can protect neurons from damage by degrading aggregates with three mechanisms, including the aggregation and removing α-Synuclein (α-Syn) aggregates, promotion the autophagy of abnormal proteins, and inhibition the apoptosis of degenerated neurons. This study underscores the importance of HSPs as targets in PD and helps to expand new mechanisms in PD treatment strategies.
- ArticleZhou J, Zou Y, Cai Y, Chi F, Huang W, Shi W, Qian H.Bioorg Chem. 2021 12;117:105453.Human epidermal growth factor receptor 2 (HER2) has been recognized as an important therapeutic target for its overexpression in many cancers. Trastuzumab is a monoclonal antibody targeting HER2, which has been approved by FDA to treat HER2-positive cancer. In this research, cyclic peptide Cyclo-GCGPep1 was designed based on the binding mode between antibody and HER2 protein in silico, which has been confirmed possessing good affinity with HER2. Cyclo-GCGPep1 was also used to construct peptide-drug conjugates with Camptothecin. Biological evaluations demonstrated that Conjugate 1 has a good antiproliferative activity on SK-BR-3 and NCI-N87 cells. Conjugate 1 retained the pro-apoptotic and Topo I inhibitory ability of Camptothecin. Meanwhile, it has good targeting ability towards HER2-positive cells with the help of Cyclo-GCGPep1. It also has better permeability in the tumor spheroid model than Camptothecin. In summary, the design of cyclic peptide derived from antibody is of significance for the discovery of targeting peptides and Conjugate 1 is expected as a good therapeutic agent for HER2-positive cancers.
- ArticleHsieh MC, Huang CH, Hsu ML.Clin Biomech (Bristol, Avon). 2021 10;89:105453.BACKGROUND: Self-tapping screws have been extensively used for dental implants. Their biomechanical behavior is highly associated with their clinical success, particularly for screws used for immediate implant placement and restoration, because occlusal forces can directly affect the loading transfer at the bone-implant interface after implantation. The effect of implant design on the initial stability of self-tapping screws remains unclear. This study explored the biomechanical behaviors of implant stability in standardized implants with different design features.
METHODS: Six types of dental implants were designed using computer-aided design/computer-aided manufacturing technology, including three types of cutting flute shapes (spiral, straight, and non-self-tapping) combined with two types of screw features. Peak insertion torque values were first recorded; initial stability levels were subsequently evaluated in terms of the maximum force and resistance to lateral loads using an electrodynamic test system.
FINDINGS: The peak insertion torque values, maximum force, and resistance to lateral loads of the non-self-tapping groups were higher than those of the self-tapping groups by 17%-90% (p < 0.01). The peak insertion torque values of the Straumann implant with a spiral flute was higher than that of the original straight flute by 20% (p < 0.001). However, compared with the original spiral flute, the Nobel Biocare implant with straight flute had a 23% higher maximum force (p = 0.016) and 24.5% higher resistance (p = 0.012) under lateral loading.
INTERPRETATION: Changing the flute design would affect initial implant stability. Non-self-tapping implants exhibited superior initial stability than did self-tapping implants. - ArticleGarcia W, Mendez S, Fray B, Nicolas A.Saf Sci. 2021 Dec;144:105453.This work assesses the risks of Covid-19 spread in diverse daily-life situations involving crowds of maskless pedestrians, mostly outdoors. More concretely, we develop a method to infer the global number of new infections from patchy observations, by coupling ad hoc spatial models for disease transmission via respiratory droplets to detailed field-data about pedestrian trajectories and head orientations. This allows us to rank the investigated situations by the infection risks that they present; importantly, the obtained hierarchy of risks is very largely conserved across transmission models: Street cafés present the largest average rate of new infections caused by an attendant, followed by busy outdoor markets, and then metro and train stations, whereas the risks incurred while walking on fairly busy streets are comparatively quite low. While our models only approximate the actual transmission risks, their converging predictions lend credence to these findings. In situations with a moving crowd, density is the main factor influencing the estimated infection rate. Finally, our study explores the efficiency of street and venue redesigns in mitigating the viral spread: While the benefits of enforcing one-way foot traffic in (wide) walkways are unclear, changing the geometry of queues substantially affects disease transmission risks.
- ArticleBluemling GR, Mao S, Natchus MG, Painter W, Mulangu S, Lockwood M, De La Rosa A, Brasel T, Comer JE, Freiberg AN, Kolykhalov AA, Painter GR.Antiviral Res. 2023 01;209:105453.The unprecedented magnitude of the 2013-2016 Ebola virus (EBOV) epidemic in West Africa resulted in over 11 000 deaths and spurred an international public health emergency. A second outbreak in 2018-2020 in DRC resulted in an additional >3400 cases and nearly 2300 deaths (WHO, 2020). These large outbreaks across geographically diverse regions highlight the need for the development of effective oral therapeutic agents that can be easily distributed for self-administration to populations with active disease or at risk of infection. Herein, we report the in vivo efficacy of N4-hydroxycytidine (EIDD-1931), a broadly active ribonucleoside analog and the active metabolite of the prodrug EIDD-2801 (molnupiravir), in murine models of lethal EBOV infection. Twice daily oral dosing with EIDD-1931 at 200 mg/kg for 7 days, initiated either with a prophylactic dose 2 h before infection, or as therapeutic treatment starting 6 h post-infection, resulted in 92-100% survival of mice challenged with lethal doses of EBOV, reduced clinical signs of Ebola virus disease (EVD), reduced serum virus titers, and facilitated weight loss recovery. These results support further investigation of molnupiravir as a potential therapeutic or prophylactic treatment for EVD.
- ArticleSantos D, Iop GD, Bizzi CA, Mello PA, Mesko MF, Balbinot FP, Flores EMM.Ultrason Sonochem. 2021 Apr;72:105453.Nitrocellulose is a nitrated cellulose polymer with a broad application in industry. Depending on the nitrogen content, this polymer can be used for manufacturing explosives, varnishes, clothes, and films, being considered a product of high value-added. In this work, the use of ultrasound was investigated for the intensification of nitrocellulose synthesis from microcrystalline cellulose. The ultrasound-assisted nitrocellulose synthesis (UANS) was carried out using several ultrasound systems, such as baths and cup horns, allowing the evaluation of the frequency (from 20 to 130 kHz) and delivered power (from 23 to 134 W dm-3) to the reaction medium. The following parameters were evaluated: acid mixture (H2SO4, H3PO4, CH2O2 or CH3COOH with HNO3, 2 to 14.4 mol L-1), ultrasound amplitude (10 to 70%) and reaction time (5 to 50 min). Better nitrocellulose yield (nitrogen content of 12.5% was obtained from 1 g of microcrystalline cellulose employing a cup horn system operating at 20 kHz, 750 W of nominal power with 60% of amplitude, 25 mL of acid solution (13.6 mL of 18.4 mol L-1 H2SO4 + 9.2 mL of 14.4 mol L-1 HNO3 + 2.2 mL H2O), at 30 °C for 30 min. At silent conditions (mechanical stirring ranging from 100 to 500 rpm), the nitrogen content was lower than 11.8% which demonstrate the ultrasound effects for nitrocellulose synthesis.
- ArticlePoirier S, Luong NM, Anthoine V, Guillou S, Membré JM, Moriceau N, Rezé S, Zagorec M, Feurer C, ... Show More Frémaux B, Jeuge S, Robieu E, Champomier-Vergès M, Coeuret G, Cauchie E, Daube G, Korsak N, Coroller L, Desriac N, Desmonts MH, Gohier R, Werner D, Loux V, Rué O, Dohollou MH, Defosse T, Chaillou S.Data Brief. 2020 Jun;30:105453.Data in this article provide detailed information on the diversity of bacterial communities present on 576 samples of raw pork or poultry sausages produced industrially in 2017. Bacterial growth dynamics and diversity were monitored throughout the refrigerated storage period to estimate the impact of packaging atmosphere and the use of potassium lactate as chemical preservative. The data include several types of analysis aiming at providing a comprehensive microbial ecology of spoilage during storage and how the process parameters do influence this phenomenon. The analysis includes: the gas content in packaging, pH, chromametric measurements, plate counts (total mesophilic aerobic flora and lactic acid bacteria), sensorial properties of the products, meta-metabolomic quantification of volatile organic compounds and bacterial community metagenetic analysis. Bacterial diversity was monitored using two types of amplicon sequencing (16S rRNA and GyrB encoding genes) at different time points for the different conditions (576 samples for gyrB and 436 samples for 16S rDNA). Sequencing data were generated by using Illumina MiSeq. The sequencing data have been deposited in the bioproject PRJNA522361. Samples accession numbers vary from SAMN10964863 to SAMN10965438 for gyrB amplicon and from SAMN10970131 to SAMN10970566 for 16S.
- ArticleBechmann RK, Arnberg M, Bamber S, Lyng E, Westerlund S, Rundberget JT, Kringstad A, Seear PJ, Burridge L.Aquat Toxicol. 2020 May;222:105453.Anti-parasitic drugs used in the aquaculture industry are discharged to the sea after treatment of salmon. In this study, the effects of azamethiphos (AZA) in the Salmosan® formulation and deltamethrin (DEL) in the Alpha Max® formulation, have been assessed in Northern shrimp larvae (Pandalus borealis) when administered both separately and in combination. The exposure concentrations were 100 ng/L for AZA and 2 ng/L for DEL, each representing a 1000-fold dilution of the prescribed concentrations for salmon. These two chemicals were combined at these concentrations to give a third treatment (AZA + DEL). When larvae were exposed for two hours on the first, second and third days post hatch (dph), significantly increased mortality and reduced swimming activity were observed for larvae from the DEL and combined AZA + DEL treatments 4 dph, though not in larvae from the AZA treatment. A single pulse exposure, delivered on the first day post hatch, caused similar effects on mortality and swimming activity 4 dph as the three-pulse exposure. Mortality was driven by the presence of DEL in both experiments, with no amplification or reduction of effects observed when DEL and AZA were combined. Larvae were observed for 13 days following the single pulse exposure, with food limitation introduced as an additional stressor on day 4. In the DEL and AZA + DEL treatments mortality continued to increase regardless of food level, with no larvae completing development to stage II. The overriding toxicity of DEL masked any potential effects the reduced food ration may have exerted. Swimming activity was lower for AZA treated larvae than Control larvae 13 dph, when both groups were fed daily, though no other significant changes to mortality, development to stage II, feeding rate or gene expression were observed. Food limited Control and AZA larvae had lower swimming activity and feeding rate than daily fed Control larvae, with expression of pyruvate kinase and myosin genes also downregulated. However, there was no negative effect on survival or successful development to stage II in these treatments. In addition, mesencephalic astrocyte-derived neurotropic factor was downregulated in food limited Control larvae when compared with the daily fed Controls. Results from this study together with reported estimates of dispersion plume concentrations of discharged pesticides indicate that toxic concentrations of deltamethrin could reach shrimp larvae several kilometers from a treated salmon farm.
- ArticleLewis RM, Baskaran H, Green J, Tashev S, Palaiologou E, Lofthouse EM, Cleal JK, Page A, Chatelet DS, Goggin P, Sengers BG.iScience. 2022 Dec 22;25(12):105453.The placental syncytiotrophoblast, a syncytium without cell-cell junctions, is the primary barrier between the mother and the fetus. Despite no apparent anatomical pathway for paracellular diffusion of solutes across the syncytiotrophoblast, size-dependent paracellular diffusion is observed. Here we report data demonstrating that the syncytiotrophoblast is punctuated by trans-syncytial nanopores (TSNs). These membrane-bound TSNs directly connect the maternal and fetal facing sides of the syncytiotrophoblast, providing a pathway for paracellular diffusion between the mother and fetus. Mathematical modeling of TSN permeability based on their 3D geometry suggests that 10-37 million TSNs per cm3 of placental tissue could explain experimentally observed placental paracellular diffusion. TSNs may mediate physiological hydrostatic and osmotic pressure homeostasis between the maternal and fetal circulations but also expose the fetus to pharmaceuticals, environmental pollutants, and nanoparticles.
- ArticlePhilippou E, Nikiphorou E.Joint Bone Spine. 2022 11;89(6):105453.
- ArticleOhta T, Kojima N, Osuka Y, Sasai H.Arch Gerontol Geriatr. 2024 09;124:105453.BACKGROUND: Physical frailty and cognitive decline are common issues in geriatrics within an aging society, yet the association between them remains controversial. This study aims to evaluate the association between physical frailty and cognitive decline among community-dwelling older Japanese women.
METHODS: A prospective cohort study was conducted in an urban area of Tokyo, Japan, involving community-dwelling older adults 65 years or older. Physical frailty was evaluated using the Japanese version of the Cardiovascular Health Study criteria, consisting of five components: shrinking, weakness, exhaustion, slowness, and low activity. Participants were classified as robust, pre-frail, or frail based on the presence of one or more criteria. Cognitive decline was defined as at least a three-point decrease in the Mini-Mental State Examination score from baseline. The association between physical frailty and cognitive decline was examined using binomial logistic regression, adjusting for potential confounders. The analysis yielded multivariable odds ratios (ORs) and 95 % confidence intervals (CIs), with robust participants using as the reference group.
RESULTS: A total of 2,122 individuals participated in the baseline survey, with 805 included in the analysis (mean age: 72.9 ± 5.1 years). At baseline, 363 participants were classified as pre-frail, while 32 were classified as frail. During the two-year follow-up period, 68 participants experienced cognitive decline. The multivariable OR (95 % CI) for frailty, using robust as the reference, was 3.50 (1.13, 10.80). Furthermore, a linear relationship was observed between physical frailty and cognitive decline.
CONCLUSION: Among older Japanese women, there exists a dose-response relationship between physical frailty status and cognitive decline. - ArticleZieger HL, Choquet D.Neurobiol Dis. 2021 10;158:105453.Neurodevelopmental disorders such as those linked to intellectual disabilities or autism spectrum disorder are thought to originate in part from genetic defects in synaptic proteins. Single gene mutations linked to synapse dysfunction can broadly be separated in three categories: disorders of transcriptional regulation, disorders of synaptic signaling and disorders of synaptic scaffolding and structures. The recent developments in super-resolution imaging technologies and their application to synapses have unraveled a complex nanoscale organization of synaptic components. On the one hand, part of receptors, adhesion proteins, ion channels, scaffold elements and the pre-synaptic release machinery are partitioned in subsynaptic nanodomains, and the respective organization of these nanodomains has tremendous impact on synaptic function. For example, pre-synaptic neurotransmitter release sites are partly aligned with nanometer precision to postsynaptic receptor clusters. On the other hand, a large fraction of synaptic components is extremely dynamic and constantly exchanges between synaptic domains and extrasynaptic or intracellular compartments. It is largely the combination of the exquisitely precise nanoscale synaptic organization of synaptic components and their high dynamic that allows the rapid and profound regulation of synaptic function during synaptic plasticity processes that underlie adaptability of brain function, learning and memory. It is very tempting to speculate that genetic defects that lead to neurodevelopmental disorders and target synaptic scaffolds and structures mediate their deleterious impact on brain function through perturbing synapse nanoscale dynamic organization. We discuss here how applying super-resolution imaging methods in models of neurodevelopmental disorders could help in addressing this question.
- ArticleHassam M, Shamsi JA, Khan A, Al-Harrasi A, Uddin R.Comput Biol Med. 2022 06;145:105453.The new and novel drug molecules are of prime importance against the deadly Mycobacterium tuberculosis owing to its high resistance. The discovery of new drug molecules is cost, time, and efforts intensive in chemical research. Computational approaches, such as virtual screening and Machine Learning represent an effective alternate to predict the active compounds with appreciable accuracy. In this work, we used the true active and in-active drug candidates to train the machine learned models against one of the potent drug targets from Mycobacterium tuberculosis i.e. Pantothenate synthetase (PS). We computed 1444 descriptors from the studied molecules. Initially, twenty descriptors were shortlisted based on their significant Pearson's correlation with the -logIC50 values. Different combinations of descriptors were used to optimize the number of descriptors. Further to that different Machine Learned models were applied to develop a trained model of active molecules with a reasonable accuracy. The best performed model in terms of prediction of the activity data is proposed as a model of choice to perform the data screening experiments. The current study will help to potentiate the drug discovery process against Mycobacterium tuberculosis (Mtb).
- ArticleRocha GD, Nogueira JF, Gomes Dos Santos MV, Boaventura JA, Nunes Soares RA, José de Simoni Gouveia J, Matiuzzi da Costa M, Gouveia GV.Microb Pathog. 2022 Apr;165:105453.There is not a consensus between the presence of the genotypic resistance marker gene and the phenotypic resistance to β-lactams in Staphylococcus aureus, which means, positive S. aureus blaZ isolates demonstrating sensitivity to β-lactams. The present study aimed to characterize the blaZ, blaR1 and blaI genes, identify and evaluate single nucleotide polymorphisms (SNPs) and their relationship with β-lactam resistance in samples of Staphylococcus aureus obtained from cases of bovine mastitis. Five isolates (two resistant and three sensitive to oxacillin) of Staphylococcus aureus with detected production of beta-lactamase, previously evaluated as containing the blaZ gene and negative for the mecA and mecC genes, had the bla operon completely sequenced. Impacts on the protein sequence due to the detected polymorphisms were evaluated by modeling the proteins encoded by the blaZ, blaR1 and blaI genes using a three-dimensional model structure obtained from the Protein Data Bank (PDB) database. Fifteen SNPs were detected in the blaZ gene, 30 in the blaR1 gene and three in the blaI gene. These SNPs caused alterations in amino acid sites. Deleterious mutations were detected in the blaZ gene (E146G, P218S, Y221C) and the blaR1 gene (K481E). Molecular docking analysis revealed that polymorphisms in the blaZ gene may explain the phenotypic sensitivity in isolates that contain the resistance marker gene. Although sensitive and resistant isolates encode beta-lactamase, these proteins are functionally altered due to a change in the binding site with the antibiotic.
- ArticleDu C, Peng P, Guo X, Wu Y, Zhang Z, Hao L, Zhang Z, Xiong J.J Dent. 2024 Nov 12;152:105453.OBJECTIVES: To propose and validate a minimally invasive combined static and dynamic computer assisted implant surgery (CAIS) workflow for zygomatic implant (ZI) placement.
METHODS: A combined approach leveraging static CAIS for initial positioning, complemented by dynamic CAIS for real-time control of the angle, depth and width was proposed. Fourteen consecutive patients (age: 60.3±9.8 years; 8 females) seeking ZI-supported restoration were enrolled. A single anatomically and prosthetically driven ZI on either the unilateral zygoma or bilateral zygomata was planned and placed using the proposed approach. The zygomatic anatomy-guided approach (ZAGA) type and the ZI length were recorded. The angular, coronal global, and apical global deviation between the planned and placed positions were measured by overlapping post- and pre-operative cone beam computer tomography. Comparisons were made between the left and right sides across the ZAGA type and ZI length. Statistical significance was set at P<0.05.
RESULTS: 22 ZIs were placed using the combined approach and 13 immediate loading prostheses were delivered, with one patient restored 6 months after surgery. The angular deviations and coronal global deviations were 1.99±0.17° and 1.21±0.45 mm, respectively. The median apical global deviation was 1.67 mm (interquartile range [IQR]: 1.11-1.93 mm). No significant differences were found between the left and right sides across the ZAGA type or ZI length. All ZIs remained stable over a median follow-up of 14.5 months (IQR: 7-20 months).
CONCLUSIONS: The proposed combination of static and dynamic CAIS is safe, reliable, accurate, and robust for ZI placement.
CLINICAL SIGNIFICANCE: This pilot study proposed a minimally invasive ZI placement method that combined static and dynamic computer-guided surgery. The implant positioning accuracy achieved using this approach validated its safety, reliability, accuracy, and robustness. The combined approach may reduce the technique sensitivity of ZI placement, facilitating future rehabilitation of severely atrophic or defective maxillae.