Live Chat
Today's Hours: 8:00am - 8:00pm
Lane Medical Library

Search

Filter Results

Did You Mean:

Search Results

Sort by
relevance
  • Article
    Santos AN, Kherif F, Melie-Garcia L, Lutti A, Chiappini A, Rauschenbach L, Dinger TF, Riess C, El Rahal A, Darkwah Oppong M, Sure U, Dammann P, Draganski B.
    Neuroimage Clin. 2023;38:103432.
    There is an ongoing debate about differential clinical outcome and associated adverse effects of deep brain stimulation (DBS) in Parkinson's disease (PD) targeting the subthalamic nucleus (STN) or the globus pallidus pars interna (GPi). Given that functional connectivity profiles suggest beneficial DBS effects within a common network, the empirical evidence about the underlying anatomical circuitry is still scarce. Therefore, we investigate the STN and GPi-associated structural covariance brain patterns in PD patients and healthy controls. We estimate GPi's and STN's whole-brain structural covariance from magnetic resonance imaging (MRI) in a normative mid- to old-age community-dwelling cohort (n = 1184) across maps of grey matter volume, magnetization transfer (MT) saturation, longitudinal relaxation rate (R1), effective transversal relaxation rate (R2*) and effective proton density (PD*). We compare these with the structural covariance estimates in patients with idiopathic PD (n = 32) followed by validation using a reduced size controls' cohort (n = 32). In the normative data set, we observed overlapping spatially distributed cortical and subcortical covariance patterns across maps confined to basal ganglia, thalamus, motor, and premotor cortical areas. Only the subcortical and midline motor cortical areas were confirmed in the reduced size cohort. These findings contrasted with the absence of structural covariance with cortical areas in the PD cohort. We interpret with caution the differential covariance maps of overlapping STN and GPi networks in patients with PD and healthy controls as correlates of motor network disruption. Our study provides face validity to the proposed extension of the currently existing structural covariance methods based on morphometry features to multiparameter MRI sensitive to brain tissue microstructure.
    Digital Access Access Options
  • Article
    Jeggo PA.
    DNA Repair (Amst). 2023 01;121:103432.
    Digital Access Access Options
  • Article
    Weijs ML, Ho JT, Roel Lesur M, Lenggenhager B.
    Conscious Cogn. 2022 11;106:103432.
    In body integrity dysphoria (BID), otherwise healthy individuals feel like a part of their physical body does not belong to them despite normal sensorimotor functioning. Theoretical and empirical evidence suggested aweakened integration of the affected body part into higher-order multisensory cortical body networks. Here, we used a multisensory stimulation paradigm in mixed reality to modulate and investigate multisensory processing underlying body (dis)ownership in individuals with BID of the lower limb. In 20 participants with BID, delay perception and body ownership were measured after introducing delays between the visual and tactile information of viewed stroking applied to affected and unaffected body parts. Unlike predicted, delay perception did not differ between the two body parts. However, specifically for the affected limb, ownership was lower and more strongly modulated by delay. These findings might be following the idea of a stronger dependency on online bottom up sensory signals in BID.
    Digital Access Access Options
  • Article
    Shorey S, Loh DNL, Chan V, Chua C, Choolani MA.
    Midwifery. 2022 Oct;113:103432.
    BACKGROUND: Contents and mode of delivery of antenatal educational programs differed considerably. Yet there is a lack of high-level evidence about the delivery of these programs.
    OBJECTIVE: We aimed to understand the experiences and needs of parents who have attended antenatal educational programs.
    DESIGN: Six databases were searched from each database. Included studies were appraised using the Critical Appraisal Skills Program tool. Qualitative data were meta-summarized and meta-synthesized.
    FINDINGS: Seventeen studies were included, and three themes were developed: (1) Contradicting views on antenatal educational programs, (2) Feeling 'well prepared' after attending the antenatal educational programs, and (3) Parents' expectations and way forward for the antenatal educational programs.
    DISCUSSION: Findings revealed that the description of contents of antenatal educational programs needed to be more specific. Mindfulness strategies were well-received by parents in the included studies. Educators should take into account inclusivity and increase educational resources related to individual, cultural and community needs. Learning needs can be assessed before and after classes. Parents with specific needs that were not addressed should be identified and referred to the relevant professionals for continued support. More deliberate actions were needed during the programs to foster social and professional networks for attendees to support them throughout antenatal and postnatal periods.
    CONCLUSION: We consolidated the experiences and needs of parents who have attended antenatal educational programs. Findings can help refine policies related to antenatal care to improve pregnancy, birth and parenthood experiences for both mothers and fathers.
    Digital Access Access Options
  • Article
    Raittinen PVH, Syvälä H, Tammela TLJ, Häkkinen MR, Ilmonen P, Auriola S, Murtola TJ.
    EBioMedicine. 2021 Jun;68:103432.
    BACKGROUND: Prostate cancer (PCa) progression depends on androgen receptor activity. Cholesterol is required for biosynthesis of all steroid hormones, including androgens. Impact of cholesterol-lowering statins on androgens is unknown. We explored atorvastatin influence on serum and prostatic tissue steroidomic profiles (SP) to expose novel pathways for limiting androgen concentration in men with PCa.
    METHODS: This is a pre-planned post hoc analysis of ESTO-1 pilot randomised, double-blinded, clinical trial. Statin naïve men, scheduled for radical prostatectomy due to localised PCa, were randomised 1:1 to use daily 80 mg of atorvastatin or placebo before the surgery for a median of 28 days. Participants were recruited and treated at the Pirkanmaa Hospital District, Tampere, Finland. 108 of the 158 recruited men were included in the analysis based on sample availability for hormone profiling. Serum and prostatic tissue steroid profiles were determined using liquid chromatography mass spectrometry. Wilcoxon rank sum test and bootstrap confidence intervals (CI) were used to analyse the difference between placebo and atorvastatin arms.
    FINDINGS: Most serum and prostatic steroids, including testosterone and dihydrotestosterone, were not associated with atorvastatin use. However, atorvastatin use induced serum SP changes in 11-ketoandrostenedione (placebo 960pM, atorvastatin 617.5pM, p-value <0.0001, median difference -342.5; 95% CI -505.23 - -188.98). In the prostatic tissue, atorvastatin was associated with plausible downshift in 11- ketodihydrotestosterone (placebo 25.0pM in 100 mg tissue/1 mL saline, atorvastatin 18.5pM in 100 mg tissue/1 mL saline, p-value 0.027, median difference -6.53; 95% CI -12.8 - -0.29); however, this association diminished after adjusting for multiple testing. No serious harms were reported.
    INTERPRETATION: Atorvastatin was associated with adrenal androgen downshift in the serum and possibly in the prostate. The finding warrants further investigation whether atorvastatin could improve androgen deprivation therapy efficacy.
    FUNDING: Funded by grants from the Finnish Cultural Foundation, Finnish Cancer Society, Academy of Finland, and the Expert Responsibility Area of the Tampere University Hospital. CLINICALTRIALS.
    GOV IDENTIFIER: NCT01821404.
    Digital Access Access Options
  • Article
    Griffith LJ, Newcombe RG, Daly S, Seong J, Davies M, West NX.
    J Dent. 2020 09;100:103432.
    OBJECTIVES: To design and validate a new index to assess tooth wear (TW) in the cervical region and its association with gingival recession (GR), for use both in research studies and as resource in General Dental Practice, with focused prevention and management strategiesparticularly related to aetiology of dentine hypersensitivity provided for each Code score.
    METHODS: Codes to reflect clinical presentations of the cervical region in health and disease recording both TW and GR were defined. Validation of the Code was undertaken by 3 trained examiners who scored buccal and lingual surfaces of eligible teeth in 42 adult healthy volunteers. Each volunteer underwent 4 identical clinical examinations, being assessed twice by the examiner who performed the first and last exam.
    RESULTS: Cervical Localisation Code definitions were A: no GR, no coronal TW, B: no GR, distinct coronal TW, C: GR but no coronal TW, D: GR with distinct coronal and root TW. For validation 2073 eligible tooth surfaces were scored. There were only 57 within and 201 between examiner disagreements, the most common being between scores C and D.
    CONCLUSIONS: The Cervical Localisation Code was used reproducibly by three independent examiners. It will provide focussed data on the cervical region in research studies allowing the association of patient reported dietary and lifestyle factors with specific lesion types, and a tool to aid the management of clinical scenarios, specifically those that lead to dentine hypersensitivity in general dental practice.
    CLINICAL SIGNIFICANCE: Exposure of dentine at the cervical margin by TW and/or GR often results in dentine hypersensitivity, however current TW indices do not record TW location or GR presence. The Cervical Localisation Code captures both parameters and suggests likely aetiology for dentine hypersensitivity and guide clinical management of the cervical region.
    Digital Access Access Options
  • Article
    Nekvinda J, Różycka D, Rykowski S, Wyszko E, Fedoruk-Wyszomirska A, Gurda D, Orlicka-Płocka M, Giel-Pietraszuk M, Kiliszek A, Rypniewski W, Bachorz R, Wojcieszak J, Grüner B, Olejniczak AB.
    Bioorg Chem. 2020 01;94:103432.
    The development of 1,8-naphthalimide derivatives as DNA-targeting anticancer agents is a rapidly growing area and has resulted in several derivatives entering into clinical trials. One of original recent developments is the use of boron clusters: carboranes and metallacarboranes in the design of pharmacologically active molecules. In this direction several naphthalimide-carborane and metallacarborane conjugates were synthesized in the present study. Their effect on a cancer cell line - cytotoxicity, type of cell death, cell cycle, and ROS production were investigated. The tested conjugates revealed different activities than the leading members of the naphthalimides family, namely mitonafide and pinafide. These derivatives could induce G0/G1 arrest and promote mainly apoptosis in HepG2 cell line. Our investigations demonstrated that the most promising molecule is N-{[2-(3,3'-commo-bis(1,2-dicarba-3-cobalta(III)-closo-dodecaborate-1-yl)ethyl]-1'-aminoethyl)}-1,8-naphthalimide] (17). It was shown that 17 exhibited cytotoxicity against HepG2 cells, activated cell apoptosis, and caused cell cycle arrest in HepG2 cells. Further investigations in HepG2 cells revealed that compound 17 can also induce ROS generation, particularly mitochondrial ROS (mtROS), which was also proved by increased 8-oxo-dG level in DNA. Additionally to biological assays the interaction of the new compounds with ct-DNA was studied by CD spectra and melting temperature, thus demonstrating that these compounds were rather weak classical DNA intercalators.
    Digital Access Access Options
  • Article
    Sreetharan S, Schlesinger JJ, Schutz M.
    Appl Ergon. 2021 Oct;96:103432.
    Auditory alarms offer great potential for facilitating human-computer interactions in complex, rapidly changing environments. They are particularly useful in medical settings, where in theory they should afford communication in emergency rooms, operating theatres, and hospitals around the world. Unfortunately, the sounds typically used in these devices are problematic, and researchers have documented numerous shortcomings. Their ubiquity means that even incremental improvements can have significant benefits for patient care. However, solutions have proven challenging for multiple reasons-including issues of backward compatibility inherent in changing any standard. Here we present a series of three experiments showing that manipulations to one specific, understudied property can significantly lower alarm annoyance without harming learning or memory-while preserving an alarm's melodic and rhythmic structure. These results suggest promising new directions for improving the hospital's soundscape, where evidence of problems related to sound are increasingly recognized as affecting medical outcomes as well as physician well-being.
    Digital Access Access Options
  • Article
    Li Y, Zhang Y, Zhang L, Hu Y, Hong C, Xie A, Wu Y, Shangguan Z, Zhou B, Fang L, Mei L.
    Food Microbiol. 2020 Aug;89:103432.
    Pathogenic Cronobacter species are responsible for life-threatening illness in neonates. A ten-year comprehensive survey was conducted to examine the population structure and antimicrobial resistant patterns of Cronobacter isolates from food (n = 78) and clinical (n = 12) sources in Wenzhou, China. A total of 90 (4.4%) isolates were recovered from 2051 collected samples. The occurrence of Cronobacter spp. was highest in spices with a rate of 22% (26/119), whereas the lowest contamination rate of 1% was found in powered infant and toddler formula (7/494), special medical infant formula (1/95) and human stool samples (12/1024). Cronobacter strains revealed a high degree of genetic diversity among the isolates tested. Pulsed-field gel electrophoresis (PFGE) distinguished 75 clonal groups, and the biggest cluster consisted of four strains. Multilocus sequence typing (MLST) method displayed 43 sequence types (STs), of which ST1, ST4, ST8, ST64, ST148 and ST201 were most frequently identified. Meanwhile, two new sequence types were discovered and added to the PubMLST international database. Resistance to ceftriaxone, cefotaxiv, amoxicillin, ampicillin, cefoxitin, tetracycline, streptomycin, azithromycin, chloramphenicol, as well as multidrug resistance, was noted. Taken together, this large-scale surveillance study highlights the wide dissemination and diverse molecular features of Cronobacter spp. in Wenzhou China.
    Digital Access Access Options
  • Article
    Gao Y, Liu Y, Liu Z.
    iScience. 2021 Dec 17;24(12):103432.
    Chemical vapor deposition (CVD) is a promising approach for the controllable synthesis of two-dimensional (2D) materials. Many studies have demonstrated that the morphology and structure of 2D materials are highly dependent on growth substrates. Hence, the choice of growth substrates is essential to achieve the precise control of CVD growth. Noble metal substrates have attracted enormous interest owing to the high catalytic activity and rich surface morphology for 2D material growth. In this review, we introduce recent progress in noble metals as substrates for the controllable growth of 2D materials. The underlying growth mechanism and substrate designs of noble metals based on their unique features are thoroughly discussed. In the end, we outline the advantages and challenges of using noble metal substrates and prospect the possible approaches to extend the uses of noble metal substrates for 2D material growth and enhance the structural controllability of the grown materials.
    Digital Access Access Options
  • Article
    Yan J, Wang R, Tan J.
    Drug Discov Today. 2023 02;28(2):103432.
    Mutations in and dysregulation of long non-coding RNAs (lncRNAs) are closely associated with the development of various human complex diseases, but only a few lncRNAs have been experimentally confirmed to be associated with human diseases. Predicting new potential lncRNA-disease associations (LDAs) will help us to understand the pathogenesis of human diseases and to detect disease markers, as well as in disease diagnosis, prevention and treatment. Computational methods can effectively narrow down the screening scope of biological experiments, thereby reducing the duration and cost of such experiments. In this review, we outline recent advances in computational methods for predicting LDAs, focusing on LDA databases, lncRNA/disease similarity calculations, and advanced computational models. In addition, we analyze the limitations of various computational models and discuss future challenges and directions for development.
    Digital Access Access Options
  • Article
    Wu H, Xing Y, Ge W, Liu X, Zou J, Zhou C, Liao J.
    J Biomed Inform. 2020 06;106:103432.
    Adverse events caused by drug-drug interaction (DDI) not only pose a serious threat to health, but also increase additional medical care expenditure. However, despite the emergence of many excellent text mining-based DDI classification methods, achieving a balance between using simpler method and better model performance is still unsatisfactory. In this article, we present a deep learning method of stacked bidirectional Gated Recurrent Unit (GRU)- convolutional neural network (SGRU-CNN) model which apply stacked bidirectional GRU (BiGRU) network and convolutional neural network (CNN) on lexical information and entity position information respectively to conduct DDIs extraction task. Furthermore, SGRU-CNN model assigns the weights of each word feature to improve performance with one attentive pooling layer. On the condition that other values are not inferior to other algorithms, experimental results on the DDI Extraction 2013 corpus show that our model achieves a 1.54% improvement in recall value. And the proposed SGRU-CNN model reaches great performance (F1-score: 0.75) with the fewest features, indicating an excellent balance between avoiding redundant preprocessing task and higher accuracy in relation extraction on biomedical literature using our method.
    Digital Access Access Options
  • Article
    Sammoni A, Mohamad O, Abdalah A, Alghazali MB, Al-Aissami M.
    Ann Med Surg (Lond). 2022 Mar;75:103432.
    Introduction: Necrotizing fasciitis [NF] is a deep soft tissue infection with high morbidity and mortality. The diagnosis is mainly clinical, and is confirmed during the surgical exploration, which is also the cornerstone of treatment.
    Case presentation: We report a case of a 16-year-old female who complained of pain, tenderness, and erythema along her abdomen and back after a minor trauma on her left palm. The patient was treated with systemic antibiotics and daily surgical debridement followed by placement of a bilateral suction drainage system. A split-thickness graft was used to cover the defect on the lower back of the patient.
    Conclusion: Sealed irrigation technique is an effective method to manage NF. Furthermore, it reduces the hospitalization duration by continuously removing the necrotic and infected material that hinder tissue healing.
    Digital Access Access Options
  • Article
    Mori M, Yoshii S, Noguchi M, Takagi D, Shimizu T, Ito H, Matsuo-Takasaki M, Nakamura Y, Takahashi S, Hamada H, Ohnuma K, Shiohama T, Hayashi Y.
    Stem Cell Res. 2024 Jun;77:103432.
    Rett syndrome is characterized by severe global developmental impairments with autistic features and loss of purposeful hand skills. Here we show that human induced pluripotent stem cell (hiPSC) lines derived from four Japanese female patients with Rett syndrome are generated from peripheral blood mononuclear cells using Sendai virus vectors. The generated hiPSC lines showed self-renewal and pluripotency and carried heterozygous frameshift, missense, or nonsense mutations in the MECP2 gene. Since the molecular pathogenesis caused by MECP2 dysfunction remains unclear, these cell resources are useful tools to establish disease models and develop new therapies for Rett syndrome.
    Digital Access Access Options
  • Article
    Löb S, Ochmann B, Ma Z, Vilsmaier T, Kuhn C, Schmoeckel E, Herbert SL, Kolben T, Wöckel A, Mahner S, Jeschke U.
    J Reprod Immunol. 2021 11;148:103432.
    BACKGROUND: A successful pregnancy is a unique and complex immunological state. Cytokines seem to be crucial for the implementation of a tolerogenic environment at the feto-maternal interphase towards the semi-allogenic fetus. Importantly, the switch from a Th1- to a Th2 cytokine profile might play a key role. Interestingly, Interleukin-18 (IL-18) can induce either Th1 or Th2 immune response depending on the local cytokine environment. Therefore, this study investigates the expression of IL-18 in early pregnancy loss.
    PATIENTS AND METHODS: The TaqMan® Human Cytokine Network Array was carried out with placental tissue of patients with healthy pregnancies (n = 15) and recurrent miscarriage (n = 15) in order to investigate differences in IL-18 mRNA expression. Immunohistochemical staining was applied to examine the IL-18 protein expression in the syncytiotrophoblast and decidua of healthy pregnancies (n = 15), spontaneous (n = 12) and recurrent miscarriage (n = 9). The characterization of IL-18 expressing cells in the decidua was evaluated by double-immunofluorescence. Correlation analysis between IL-18 protein expression and clinical data of the study population was performed via spearman correlation coefficient.
    RESULTS: Gene expression analysis revealed a 4,9-times higher expression of IL-18 in recurrent miscarriage patients. IL-18 protein expression was significantly upregulated only in the decidua in the recurrent miscarriage group (p = 0.031). We did not observe significant changes of IL-18 protein expression in spontaneous miscarriage specimens when compared to healthy controls (p = 0.172). Double-immunofluorescence identified decidual stroma cells as IL-18 expressing cells. Correlation analysis showed a significant negative correlation of IL-18 protein expression and gestational age in healthy controls (r = -,745, p = 0.034). Also, a positive correlation of IL-18 and maternal age was observed in patients suffering from recurrent pregnancy loss (r =, 894, p = 0.041).
    CONCLUSION: Our results indicate that IL-18 expression might be necessary in early gestation but requires a tight regulation for a successful ongoing pregnancy. In the present study we observed that a significant upregulation of IL-18 in the decidua was restricted to patients with recurrent miscarriage and therefore might be interesting as a diagnostic marker. Further studies need to evaluate the exact pathophysiological mechanisms.
    Digital Access Access Options
  • Article
    Canola PA, Cardenas JJ, Sá GC, de Paula VB.
    J Equine Vet Sci. 2021 06;101:103432.
    To evaluate the feasibility of performing needle arthroscopy of the scapulohumeral joint and bicipital bursa in standing horses by using the thoracic limbs of euthanized horses. To that purpose, this experimental study was designed. Ten thoracic limbs, five left and five right from euthanized horses were retained in an upright position to simulate a standing horse. The craniolateral and lateral scapulohumeral arthroscopic portals were used to access the articular structures. A third (caudal) portal was also identified and described. The degree of difficulty in accessing the articular space and the optic maneuverability within the joint were compared between portals. The needle arthroscopy image lacks in quality, but the procedure was considered useful as a diagnostic aid for examination of the scapulohumeral joint of horses. Needle arthroscopy of the bicipital bursa was not possible. Needle arthroscopy has a great diagnostic potential for lesions in the scapulohumeral joint of horses. A new access (caudal) to the scapulohumeral joint of horses has been identified.
    Digital Access Access Options
  • Article
    Sharma K, Ghosh A, Krishnan NC, Kathirvel S, Basu D, Kumar A, George BB.
    Asian J Psychiatr. 2023 Mar;81:103432.
    BACKGROUND: Adolescence and early adulthood are vulnerable periods for substance use-related disorders later in life. The use of internet-enabled interventions can be useful, especially in low-resource settings.
    AIMS: To examine the feasibility, acceptability, and preliminary effectiveness of single-session digital screening and brief intervention (d-SBI) for illicit drug misuse in college students and explore barriers and facilitators of d-SBI.
    METHODS: Design: Mixed-methods, pilot cluster randomized trial.
    SETTING: Four conveniently selected colleges were randomized into intervention and control groups.
    PARTICIPANTS: 219 students were screened, and 37 fulfilled eligibility. Twenty-four completed follow-ups. In-depth interviews were done with ten students. Intervention and Comparator: Following a digital screening, Alcohol, Smoking, and Substance Involvement Screening Test (ASSIST) based brief intervention was provided in the d-SBI group. The control group received brief education.
    MEASUREMENTS: Acceptability was assessed by direct questions and usage statistics. ASSIST scores of groups were assessed at baseline and 3 months. Inductive coding of the interview transcript was done.
    RESULTS: More than 50 % of participants found d-SBI user-friendly, appropriate, and useful. Eighty percent of users, who logged in, completed screening. Per-protocol analysis showed a reduction in cannabis-ASSIST score over 3 months. The mean ASSIST score for other drugs combined did not differ significantly between groups. The difference in risk transition (moderate to low) was not significant. Qualitative analysis revealed three overarching themes- recruitment, engagement, and behavior change.
    CONCLUSIONS: Digital SBI for drug misuse is feasible among college students. d-SBI might be effective in reducing cannabis use.
    Digital Access Access Options
  • Article
    Cardona-Botero VE, Lara-Reséndiz RA, Woolrich-Piña GA, Pineda E, Lira-Noriega A, Gadsden H.
    J Therm Biol. 2023 Feb;112:103432.
    There is strong covariation between the thermal physiology of ectothermic animals and their thermal environment. Spatial and temporal differences in the thermal environment across a species' range may result in changes in thermal preferences between populations of that species. Alternatively, thermoregulatory-based microhabitat selection can allow individuals to maintain similar body temperatures across a broad thermal gradient. Which strategy a species adopts is often dependent on taxon-specific levels of physiological conservatism or ecological context. Identifying which strategies species use in response to spatial and temporal variation in environmental temperatures requires empirical evidence, which then can support predictions as to how a species might respond to a changing climate. Here we present findings of our analyses of the thermal quality, thermoregulatory accuracy and efficiency for the lizard, Xenosaurus fractus, across an elevation-thermal gradient and over the temporal thermal variation associated with seasonal changes. Xenosaurus fractus is a strict crevice-dweller, a habitat that can buffer this lizard from extreme temperatures and is a thermal conformer (body temperatures reflect air and substrate temperatures). We found populations of this species differed in their thermal preferences along an elevation gradient and between seasons. Specifically, we found that habitat thermal quality, thermoregulatory accuracy and efficiency (all measures of how well the lizards' body temperatures compared to their preferred body temperatures) varied along thermal gradients and with season. Our findings indicate that this species has adapted to local conditions and shows seasonal flexibility in those spatial adaptations. Along with their strict crevice-dwelling habitat, these adaptations may provide some protection against a warming climate.
    Digital Access Access Options
  • Article
    Angsujinda K, Peala W, Sittidech A, Wanganurakkul S, Mahony TJ, Wang SF, Smith DR, Chintapitaksakul L, Khongchareonporn N, Assavalapsakul W.
    Poult Sci. 2024 Mar;103(3):103432.
    Significant challenges to poultry health are posed by chicken anemia virus (CAV), which induces immunosuppression and causes increased susceptibility to secondary infections. The effective management and containment of CAV within poultry stocks require precise and prompt diagnosis. However, a deficiency persists in the availability of low-cost, rapid, and portable CAV detection devices. In this study, an immunochromatographic lateral-flow test strip-based assay was developed for CAV detection using in-house generated monoclonal antibodies (MABs) against CAV viral protein 1 (VP1). The recombinant truncated VP1 protein (Δ60VP1), with amino acid residues 1 to 60 of the native protein deleted, was produced via a prokaryotic expression system and utilized for immunizing BALB/c mice. Subsequently, high-affinity MABs against Δ60VP1 were generated and screened using conventional hybridoma technology combined with serial dilution assays. Two MABs, MAB1, and MAB3, both binding to distinct epitopes of Δ60VP1, were selected for the development of a lateral-flow assay. Sensitivity analysis demonstrated that the Δ60VP1 antigen could be detected by our homemade lateral-flow assay at concentrations as low as 625 ng/mL, and this sensitivity was maintained for at least 6 mo. The assay exhibited high specificity, as evidenced by its lack of reactivity with surrogate recombinant proteins and the absence of cross-reactivity with other chicken viruses and viral antigens. Comparative analysis with quantitative PCR data demonstrated substantial agreement, with a Kappa coefficient of 0.66, utilizing a sample set comprising 305 clinical chicken serum samples. In conclusion, the first lateral-flow assay for CAV detection was developed in this study, utilizing 2 specific anti-VP1 MABs. It is characterized by simplicity, rapidity, sensitivity, and specificity.
    Digital Access Access Options
  • Article
    Deng X, Lan T, Chen Z, Zhang M, Tao Q, Lu Z.
    Comput Biol Med. 2019 11;114:103432.
    BACKGROUND: Parotid ducts (PDs) play an important role in the diagnosis and treatment of parotid lesions. Segmentation of PDs from Cone beam computed tomography (CBCT) images has a significant impact to the pathological analysis of the parotid gland. Although level set methods (LSMs) have achieved considerable success in medical imaging segmentation, it is still a challenging task for existing LSMs to precisely and self-adaptively segment PDs from parotid duct (PD) images with both noise, intensity inhomogeneity, and vague boundary. In this paper, we propose a novel Self-adaptive Weighted level set method via Local intensity Difference (SWLD) to comprehensively solve the above issues.
    METHOD: Firstly, a new adaptive weighted operator based on local intensity variance difference has been proposed to overcome the limitations of previous LSMs that are sensitive to parameters, which achieves the aim of automatic segmentation. Secondly, we introduce local intensity mean difference into the energy function to improve the curve evolution efficiency. Thirdly, we eliminate the effects of intensity inhomogeneity, noise, and boundary blur in the parotid image through a local similarity factor with two different neighborhood sizes.
    RESULTS: Using the same dataset, segmentation of PDs is performed using the proposed SWLD algorithm and existing LSM algorithms. The mean Dice score for the proposed algorithm is 91.3%, and the corresponding mean Hausdorff distance (HD) is 1.746.
    CONCLUSION: Experimental results demonstrate that the proposed algorithm is superior to many existing level set segmentation algorithms, and it can accurately and automatically segment the PDs even in complex gradient boundaries.
    Digital Access Access Options