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  • Article
    Zhang C, Wang Y, Feng Y, Zhang Y, Ji B, Wang S, Sun Y, Zhu C, Zhang D, Sun Y.
    Oncotarget. 2016 Dec 27;7(52):86134-86147.
    Colorectal cancer(CRC) is one of the most commonly diagnosed cancers in human beings and metastasis is the main death reason. Recently, Gli1 has been reported to be a key regulator of various cancer biologies and genes expressions. However, the detailed molecular mechanism of Gli1 in CRC metastasis remains largely unknown. In this study, we aimed to investigate the role of Gli1 in CRC metastasis. We used qRT-PCR, Immunohistochemistry and Western blot to test the expression levels of Gli1, Foxm1 and other target genes in the tissues and cells; Lentivirus stable transfection to change the expression levels of Gli1 and Foxm1; Wound-healing, cell invasion, migration assays and tail vein metastatic assay to test the role of Gli1 in CRC metastasis in vitro and vivo. We demonstrated that Gli1 was significantly overexpressed in colorectal cancer tissues and cells. Foxm1 level had a positive correlation with Gli1. Furthermore, we found that Gli1 promotes colorectal cancer cells metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling. Thus, we proved that Gli1 plays important role in CRC metastasis and provided a new visual field on the therapy of CRC metastasis.
    Digital Access Access Options
  • Article
    Ebeling S, Naumann K, Pollok S, Wardecki T, Vidal-Y-Sy S, Nascimento JM, Boerries M, Schmidt G, Brandner JM, Merfort I.
    PLoS One. 2014;9(1):e86147.
    BACKGROUND: Birch bark has a long lasting history as a traditional medicinal remedy to accelerate wound healing. Recently, the efficacy of birch bark preparations has also been proven clinically. As active principle pentacyclic triterpenes are generally accepted. Here, we report a comprehensive study on the underlying molecular mechanisms of the wound healing properties of a well-defined birch bark preparation named as TE (triterpene extract) as well as the isolated single triterpenes in human primary keratinocytes and porcine ex-vivo wound healing models.
    METHODOLOGY/PRINCIPAL FINDINGS: We show positive wound healing effects of TE and betulin in scratch assay experiments with primary human keratinocytes and in a porcine ex-vivo wound healing model (WHM). Mechanistical studies elucidate that TE and betulin transiently upregulate pro-inflammatory cytokines, chemokines and cyclooxygenase-2 on gene and protein level. For COX-2 and IL-6 this increase of mRNA is due to an mRNA stabilizing effect of TE and betulin, a process in which p38 MAPK and HuR are involved. TE promotes keratinocyte migration, putatively by increasing the formation of actin filopodia, lamellipodia and stress fibers. Detailed analyses show that the TE components betulin, lupeol and erythrodiol exert this effect even in nanomolar concentrations. Targeting the actin cytoskeleton is dependent on the activation of Rho GTPases.
    CONCLUSION/SIGNIFICANCE: Our results provide insights to understand the molecular mechanism of the clinically proven wound healing effect of birch bark. TE and betulin address the inflammatory phase of wound healing by transient up-regulation of several pro-inflammatory mediators. Further, they enhance migration of keratinocytes, which is essential in the second phase of wound healing. Our results, together with the clinically proven efficacy, identify birch bark as the first medical plant with a high potential to improve wound healing, a field which urgently needs effective remedies.
    Digital Access Access Options
  • Book
    Tamara Ownsworth.
    Summary: "An injury to the brain can affect virtually any aspect of functioning and, at the deepest level, can alter sense of self or the essential qualities that define who we are. In recent years, there has been a growing body of research investigating changes to self in the context of brain injury. Developments in the cognitive and social neurosciences, psychotherapy and neurorehabilitation have together provided a rich perspective on self and identity reformation after brain injury. This book draws upon these theoretical perspectives and research findings to provide a comprehensive account of the impact of brain injury on self-identity. The second half of this book provides an in-depth review of clinical strategies for assessing changes in self-identity after brain injury, and of rehabilitation approaches for supporting individuals to maintain or re-establish a positive post-injury identity. The book emphasizes a shift in clinical orientation, from a traditional focus on alleviating impairments, to a focus on working collaboratively with people to support them to re-engage in valued activities and find meaning in their lives after brain injury. Self-Identity after Brain Injury is the first book dedicated to self-identity issues after brain injury which integrates theory and research, and also assessment and intervention strategies. It will be a key resource to support clinicians and researchers working in brain injury rehabilitation, and will be of great interest to researchers and students in clinical psychology, neuropsychology, and allied health disciplines"-- Provided by publisher.

    Contents:
    1. Overview of self-identity after brain injury
    2. What is the self? Historical and contemporary accounts
    3. Introduction to brain injury and consequences during childhood and adolescence
    4. Psychological adjustment and self-identity changes after brain injury
    5. Approaches for assessing changes to self after brain injury
    6. Individual psychotherapy and neurorehabilitation approaches
    7. Group and community-based interventions
    8. Family and paediatric Interventions
    9. Summary and future directions.
    Digital Access TandFonline 2014