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  • Article
    Kar S, Kumar H, Nc S, Mishra S, Bajoria AA, Saha M.
    Cureus. 2024 Sep;16(9):e69817.
    Introduction Eosin stain is a commonly used histological dye that selectively binds to acidic structures in cells, imparting a color between pink and red. Eosin stain can be harmful due to its chemical composition. Inhaling eosin stain in powder form or as aerosolized droplets can cause irritation in the respiratory tract. To overcome the toxic effects of eosin, many naturally available organic substitutes have been tested in histopathology laboratories, including rose extract, beetroot stain, and curcumin. These natural stain alternatives provide effective staining of tissue while ensuring minimal risk to laboratory personnel. Aim The study was conducted to evaluate the efficacy of food coloring agents over eosin stain in histopathological investigations. Materials and method The study was carried out in Kalinga Institute of Dental Sciences, Kalinga Institute of Industrial Technology (KIIT) (Deemed to be University), Bhubaneswar, India. The sample size comprised 30 oral mucosal lesion blocks. After the preparation of four micron thick sections from each block, one was stained with hematoxylin and eosin (H&E) stain and the other with a food coloring agent. Sections were kept in food color stain for three minutes. Next, the sections were dehydrated, cleared, and mounted in dibutylphthalate polystyrene xylene (DPX). To evaluate the staining with H&E and food color, each section was scored 0 for inadequate staining, one for adequate, and two for excellent staining. The tissue components stained for the study were red blood cells (RBCs), collagen fibers, muscle fibers, epithelium overall, basement membrane, cell membrane, desmosomes, keratin, cytoplasm, and nuclei. Kolmogorov-Smirnov tests were used to calculate the inferential statistics for the different variables between the groups. The distribution of the study sample was found to be not normal; therefore, a nonparametric test of significance was applied. Results All the tissue components showed excellent staining by H&E (score 2). Among the tissue components, in most of the samples, keratin, cytoplasm, and RBCs showed excellent staining (score 2) on par with the H&E stain. Other tissue components showed no staining (score 0) to adequate staining (score 1). The basement membrane and cell membrane staining were not adequate (score 0). The nuclear staining by hematoxylin was unaffected by food color and was on par with normal H&E staining. Conclusion We conclude that tomato red food color, which is non-toxic, safe for the health of laboratory personnel, easy to dispose of, and environmentally friendly, could be used as a replacement for eosin in the routine H&E techniques. Our observations could be strengthened by increasing the sample sizes and modifying the stain preparation to ensure positive staining of all tissue components, thereby enhancing the results.
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  • Article
    Baselga-Escudero L, Arola-Arnal A, Pascual-Serrano A, Ribas-Latre A, Casanova E, Salvadó MJ, Arola L, Blade C.
    PLoS One. 2013;8(7):e69817.
    miR-33 and miR-122 are major regulators of lipid metabolism in the liver, and their deregulation has been linked to the development of metabolic diseases such as obesity and metabolic syndrome. However, the biological importance of these miRNAs has been defined using genetic models. The aim of this study was to evaluate whether the levels of miR-122 and miR-33a in rat liver correlate with lipemia in nutritional models. For this purpose, we analyzed the levels of miRNA-33a and miR-122 in the livers of dyslipidemic cafeteria diet-fed rats and of cafeteria diet-fed rats supplemented with proanthocyanidins and/or ω-3 PUFAs because these two dietary components are well-known to counteract dyslipidemia. The results showed that the dyslipidemia induced in rats that were fed a cafeteria diet resulted in the upregulation of miR-33a and miR-122 in the liver, whereas the presence of proanthocyanidins and/or ω-3 PUFAs counteracted the increase of these two miRNAs. However, srebp2, the host gene of miR-33a, was significantly repressed by ω-3 PUFAs but not by proanthocyanidins. Liver mRNA levels of the miR-122 and miR-33a target genes, fas and pparβ/δ, cpt1a and abca1, respectively, were consistent with the expression of these two miRNAs under each condition. Moreover, the miR-33a and abca1 levels were also analyzed in PBMCs. Interestingly, the miR-33a levels evaluated in PBMCs under each condition were similar to the liver levels but enhanced. This demonstrates that miR-33a is expressed in PBMCs and that these cells can be used as a non-invasive way to reflect the expression of this miRNA in the liver. These findings cast new light on the regulation of miR-33a and miR-122 in a dyslipidemic model of obese rats and the way these miRNAs are modulated by dietary components in the liver and in PBMCs.
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  • Article
    Fabretti SC, Brassica SC, Cianciarullo MA, Romano-Lieber NS.
    Cad Saude Publica. 2018 09 06;34(9):e00069817.
    The study aimed to verify the application and performance of triggers for adverse drug events in hospitalized newborns. This prospective cohort study was conducted in the neonatal care units of a university hospital from March to September 2015. A list of triggers was developed for the identification of adverse drug events in this population. The list included antidote, clinical, and laboratory triggers. A total of 125 newborns who had received drugs during the hospitalization were included. Neonatal patient charts were screened to detect triggers. When a trigger was found, the patient chart was reviewed to identify possible adverse drug events. Each trigger's yield in the identification of adverse drug events was calculated and then classified according to its performance. Nine hundred and twenty-five triggers identified 208 suspected adverse drug events. The triggers' overall yield was 22.5%. The most frequently identified triggers were: drop in oxygen saturation, increased frequency of bowel movements, medications stop, and vomiting. The triggers with the best performance in the identification of adverse drug events were: increased creatinine, increased urea, necrotizing enterocolitis, prescription of flumazenil, hypercalcemia, hyperkalemia, hypernatremia, and oversedation. The triggers identified in this study can be used to track adverse drug events in similar neonatal care services, focusing on the triggers with the best performance and the lowest workload in the identification.
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  • Article
    Assar DH, Asa SA, El-Abasy MA, Elbialy ZI, Shukry M, Latif AAE, BinMowyna MN, Althobaiti NA, El-Magd MA.
    Environ Sci Pollut Res Int. 2022 Oct;29(46):69798-69817.
    Ochratoxin A (OTA) is one of the most dangerous and that pollute agricultural products, inducing a variety of toxic effects in humans and animals. The current study explored the protective effect of different concentrations of Aspergillus awamori (A. awamori) against OTA (0.3 mg/kg diet) induced renal and cardiac damage by exploring its mechanism of action in 60 New Zealand white male rabbits. Dietary supplementation of A. awamori at the selected doses of 50, 100, and 150 mg/kg diet, respectively, for 2 months significantly improved the rabbit's growth performance; modulated the suppressed immune response and restored the altered hematological parameters; reduced the elevated levels of renal injury biomarkers such as urea, creatinine, and alkaline phosphatase; and increased serum total proteins concentrations. Moreover, it also declined enzymatic activities of cardiac injury biomarkers, including AST, LDH, and CK-MB. A. awamori alleviated OTA-induced degenerative and necrotic changes in the kidney and heart of rabbits. Interestingly, A. awamori upregulated Nrf2/OH-1 signaling pathway. Therefore enhanced TAC, CAT, and SOD enzyme activities and reduced OTA-induced oxidative and nitrosative stress by declining iNOS gene expression and consequently lowered MDA and NO levels. In addition to attenuating renal and cardiac inflammation via reducing IL-1β, TNF-α gene expressions in a dose-dependent response. In conclusion,this is the first report to pinpoint that dietary incorporation of A. awamori counteracted OTA-induced renal and cardiac damage by potentiating the rabbit's antioxidant defense system through its potent antioxidant, free radical scavenging, and anti-inflammatory properties in a dose-dependent response. Based on our observations, A. awamori could be utilized as a natural protective agent against ochratoxicosis in rabbits.
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  • Article
    Geffert ZJ, Xiong Z, Grutzmacher J, Wilderman M, Mohammadi A, Filip A, Li Z, Soman P.
    ACS Appl Mater Interfaces. 2024 Dec 18;16(50):69807-69817.
    Although many lab-on-chip applications require inch-sized devices with microscale feature resolution, achieving this via current 3D printing methods remains challenging due to inherent trade-offs between print resolution, design complexity, and build sizes. Inspired by microscopes that can switch objectives to achieve multiscale imaging, we report a new optical printer coined multipath projection stereolithography (MPS) specifically designed for printing microfluidic devices. MPS is designed to switch between high-resolution (1× mode, ∼10 μm) and low-resolution (3× mode, ∼30 μm) optical paths to generate centimeter-sized constructs (3 × 6 cm) with a feature resolution of ∼10 μm. Illumination and projection systems were designed, resin formulations were optimized, and slicing software was integrated with hardware with the goal of ease of use. Using a test case of micromixers, we show that user-defined CAD models can be directly input to an automated slicing software to define printing of low-resolution features via the 3× mode with embedded microscale fins via 1× mode. A new computational model, validated using experimental results, was used to simulate various fin designs, and experiments were conducted to verify simulated mixing efficiencies. New 3D out-of-plane micromixer designs were simulated and tested. To show broad applications of MPS, multichambered chips and microfluidic devices with microtraps were also printed. Overall, MPS can be a new fabrication tool to rapidly print a range of lab-on-chip applications.
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  • Book
    Stephen L. Walston.
    Summary: "Developing and implementing strategy is one of the most challenging tasks for healthcare leaders, as it requires a wide range of skills and knowledge. Strategic Healthcare Management: Planning and Execution provides a thorough overview of strategic principles and the competencies needed to apply them, such as communication, decision making, goal setting, data analyses, project management, and financial analysis. The book emphasizes both competitive and collaborative strategies to help healthcare leaders further their organization's mission rather than merely outperform competitors. The third edition includes 10 brand-new cases and expanded content, including new chapters on: The growing trend of healthcare data analytics, with emphasis on data-driven strategic analysis. Project management principles to support strategy implementation, with an exploration of tools and techniques such as Gantt charts. The fundamental concepts and theories of strategy, as well as the actual execution and assessment of strategic plans, are all covered in this book. Readers will gain the theoretical foundation and hands-on experience they need to comprehend, apply, and assess strategies."--Vitalsource.com viewed June 14, 2023

    Contents:
    Strategy and Strategic Management
    Understanding Market Structure and Strategy
    Business Models and Common Strategies
    Growth and Integration Strategies
    Strategic Alliances
    Stakeholders, Values, Mission, and Vision
    External Environment and Strategy
    Internal Environment and Strategy
    Healthcare Analytics and Strategic Management
    Strategic Financial Analysis
    Development and Execution of a Strategic Plan
    Business Plans and Strategic Management
    Organizational Structure and Strategy
    Strategic Change Management
    Strategic Leadership
    Implementing, Monitoring, and Evaluating Strategy
    Project Planning and Management.
    Digital Access R2Library [2023], ©2023