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- ArticleÇoşkun N, Abasiyanik A.Cureus. 2024 Jan;16(1):e51618.PURPOSE: The aim of the study is to examine the follow-up and treatment results of late renal functions in children with high-grade (Grades 4, 5) renal trauma resulting from blunt abdominal injury.
METHODS: The follow-up and treatment reports of 41 patients with renal trauma admitted to our clinic between the years 2005 and 2015 were reviewed retrospectively.
RESULTS: Eight of the 41 cases had Grade 1, five had Grade 2, and 12 had Grade 3 renal trauma. The remaining 16 cases (12 of which were Grade 4, four were Grade 5) had high grade renal trauma. Four (25%) patients with high-grade renal trauma were operated (JJ stent placement was performed on one, renorraphy was performed on two, pyeloplasty and urinoma drainage were performed on one), and 12 patients were followed conservatively. In the long-term follow-up (>1 year), Tc-99m mercaptoacetyltriglycine (MAG3) kidney scintigraphy examination of three (30%) patients out of the 10 patients who were followed up conservatively, the affected kidneys were found to be nonfunctional (renal differential function 0%). The mean differential renal function in four patients who underwent surgery was 31% (between 25% and 40%).
CONCLUSION: It should be kept in mind that kidneys may become atrophic or non-functional in the late period of follow-up in cases that are followed conservatively due to high-grade renal trauma. There is no standard algorithm or treatment method in the management of high-grade renal trauma. In order to achieve a good outcome, the treatment should be individualized as much as possible. - ArticleChen MC, Hsu WL, Hwang PA, Chen YL, Chou TC.Oncotarget. 2016 08 09;7(32):51608-51618.Cancer cachexia is characterized by anorexia, skeletal muscle atrophy, and systemic inflammation. Fucoidan extracted from brown algae exhibits anti-inflammatory and anticancer activities. However, whether fucoidan ameliorates tumour and chemotherapy-induced muscle atrophy and -related cachectic symptoms remains unknown. Compared with mice with bladder cancer treated with chemotherapy alone (TGC group), those treated with a combination of low molecular weight fucoidan (LMWF) and chemotherapy drugs such as gemcitabine and cisplatin (TGCF) showed a significant reduction of body weight loss, muscle atrophy, and intestinal injury and dysfunction. Moreover, myostatin, activin A, and pro-inflammatory cytokine production, FoxO3 expression and activation, NF-κB activation, MuRF-1 and MAFbx/atrogin-1 expression, and proteasome activity in muscle were significantly decreased in the TGCF group compared with the TGC group. In addition, insulin-like growth factor 1 (IGF-1) expression and formation, and IGF-1-regulated mTOR/p70S6k/4EBP-1 protein synthesis signalling were elevated in the TGCF group compared with the TGC group. Taken together, these results suggest that LMWF is a potential agent for preventing cancer cachexia-associated muscle atrophy during chemotherapy. Furthermore, the beneficial effect of LMWF may be attributed to suppressing NF-κB-evoked inflammation, myostatin and activin A production, and subsequent muscle proteolysis, and enhancing IGF-1-dependent protein synthesis.
- ArticleLambert SL, Yang CF, Liu Z, Sweetwood R, Zhao J, Cheng L, Jin H, Woo J.PLoS One. 2012;7(12):e51618.BACKGROUND: Toll-like receptor (TLR)4 agonists are known potent immunostimulatory compounds. These compounds can be formulated as part of novel adjuvants to enhance vaccine medicated immune responses. However, the contribution of the formulation to the innate in vivo activity of TLR4 agonist compounds is not well understood.
METHODOLOGY AND PRINCIPAL FINDINGS: We evaluated synthetic TLR4 agonist Glucopyranosyl Lipid A (GLA) for its effects on molecular and cellular innate immune responses in the murine model. Microarray techniques were used to compare the responses to GLA in an aqueous formulation or in an oil-in-water Stable Emulsion formulation (GLA-SE) versus either SE alone or the mineral salt aluminum hydroxide (alum) at the muscle injection site over multiple timepoints. In contrast to the minimal gene upregulation induced by SE and alum, both GLA and GLA-SE triggered MyD88- and TRIF-dependent gene expression. Genes for chemokines, cytokine receptors, signaling molecules, complement, and antigen presentation were also strongly upregulated by GLA and GLA-SE. These included chemokines for T(H)1-type T cells (CXCL9 and CXCL10) and mononuclear leukocytes (CCL2, CCL3) among others. GLA-SE induced stronger and more sustained gene upregulation than GLA in the muscle; GLA-SE induced genes were also detected in local draining lymph nodes and at lower levels in peripheral blood. Both GLA and GLA-SE resulted in increased cellular trafficking to the draining lymph nodes and upregulated MHC molecules and ICAM1 on local dendritic cells. GLA and GLA-SE transiently upregulated circulating MCP-1, TNFα, IFNγ and IP-10 in blood.
CONCLUSIONS/SIGNIFICANCE: While GLA and GLA-SE activate a large number of shared innate genes and proteins, GLA-SE induces a quantitatively and qualitatively stronger response than GLA, SE or alum. The genes and proteins upregulated could be used to facilitate selection of appropriate adjuvant doses in vaccine formulations. - ArticleGarb JE.J Vis Exp. 2014 Nov 03(93):e51618.Venoms are chemically complex secretions typically comprising numerous proteins and peptides with varied physiological activities. Functional characterization of venom proteins has important biomedical applications, including the identification of drug leads or probes for cellular receptors. Spiders are the most species rich clade of venomous organisms, but the venoms of only a few species are well-understood, in part due to the difficulty associated with collecting minute quantities of venom from small animals. This paper presents a protocol for the collection of venom from spiders using electrical stimulation, demonstrating the procedure on the Western black widow (Latrodectus hesperus). The collected venom is useful for varied downstream analyses including direct protein identification via mass spectrometry, functional assays, and stimulation of venom gene expression for transcriptomic studies. This technique has the advantage over protocols that isolate venom from whole gland homogenates, which do not separate genuine venom components from cellular proteins that are not secreted as part of the venom. Representative results demonstrate the detection of known venom peptides from the collected sample using mass spectrometry. The venom collection procedure is followed by a protocol for dissecting spider venom glands, with results demonstrating that this leads to the characterization of venom-expressed proteins and peptides at the sequence level.
- ArticleHassan S, Gomez-Reyes E, Enciso-Martinez E, Shi K, Campos JG, Soria OYP, Luna-Cerón E, Lee MC, Garcia-Reyes I, Steakelum J, Jeelani H, García-Rivera LE, Cho M, Cortes SS, Kamperman T, Wang H, Leijten J, Fiondella L, Shin SR.ACS Appl Mater Interfaces. 2022 Nov 23;14(46):51602-51618.Recapitulating inherent heterogeneity and complex microarchitectures within confined print volumes for developing implantable constructs that could maintain their structure in vivo has remained challenging. Here, we present a combinational multimaterial and embedded bioprinting approach to fabricate complex tissue constructs that can be implanted postprinting and retain their three-dimensional (3D) shape in vivo. The microfluidics-based single nozzle printhead with computer-controlled pneumatic pressure valves enables laminar flow-based voxelation of up to seven individual bioinks with rapid switching between various bioinks that can solve alignment issues generated during switching multiple nozzles. To improve the spatial organization of various bioinks, printing fidelity with the z-direction, and printing speed, self-healing and biodegradable colloidal gels as support baths are introduced to build complex geometries. Furthermore, the colloidal gels provide suitable microenvironments like native extracellular matrices (ECMs) for achieving cell growths and fast host cell invasion via interconnected microporous networks in vitro and in vivo. Multicompartment microfibers (i.e., solid, core-shell, or donut shape), composed of two different bioink fractions with various lengths or their intravolume space filled by two, four, and six bioink fractions, are successfully printed in the ECM-like support bath. We also print various acellular complex geometries such as pyramids, spirals, and perfusable branched/linear vessels. Successful fabrication of vascularized liver and skeletal muscle tissue constructs show albumin secretion and bundled muscle mimic fibers, respectively. The interconnected microporous networks of colloidal gels result in maintaining printed complex geometries while enabling rapid cell infiltration, in vivo.
- ArticleFan X, Wei P, Li G, Li M, Lan L, Hao Q, Qiu T.ACS Appl Mater Interfaces. 2021 Nov 03;13(43):51618-51627.Efficient photoinduced charge transfer (PICT) resonance is crucial to the surface-enhanced Raman scattering (SERS) performance of metal oxide substrates. Herein, we venture into the hot-electron injection strategy to achieve unprecedented enhanced PICT efficiency between substrates and molecules. A heterojunction array composed of plasmonic MoO2 and semiconducting WO3-x is designed to prove the concept. The plasmonic MoO2 generates intense localized surface plasmon resonance under illumination, which can generate near-field Raman enhancement as well as accompanied plasmon-induced hot-electrons. The hot-electron injection in direct interfacial charge transfer and plasmon-induced charge transfer process can effectively promote the PICT efficiency between substrates and molecules, achieving a record Raman enhancement factor among metal oxide substrates (2.12 × 108) and the ultrasensitive detection of target molecule down to 10-11 M. This work demonstrates the possibility of hot-electron manipulation to realize unprecedented Raman enhancement in metal oxides, offering a cutting-edge strategy to design high-performance SERS substrates.
- Book[edited by] David Rakel, MD, Vincent Joseph Minichiello, MD.Summary: "Written by physicians who are experts in both traditional and complementary medicine, Integrative Medicine, 5th Edition, uses a clinical, disease-oriented approach to safely and effectively incorporate alternative therapies into primary care practice. Drawing on available scientific evidence and the authors' first-hand experiences, it covers therapies such as botanicals, supplements, mind-body, lifestyle choices, nutrition, exercise, spirituality, and other integrative medicine modalities. This highly regarded reference offers practical guidance for reducing costs and improving patient care while focusing on prevention and wellness for a better quality of life."--Publisher marketing.
Contents:
Philosophy of integrative medicine
Optimal healing environments
The healing encounter
The whole health approach
Clinician resilience
Depression
Anxiety
Attention-deficit/hyperactivity disorder
Autism spectrum disorder
Insomina
Posttraumatic stress disorder (PTSD)
Alzheimer dieases
Headache
Peripheral neuropathy
Multiple sclerosis
Parkinson disease
Otitis media
Chronic sinusitis
Acute respiratory infection cold and flu
HIV disease and AIDS
Herpes simplex virus
Chronic hepatitis
Urinary tract infection (UTI)
Lyme disease
Non-pharmaceutical approaches to hypertension
Heart failure
Coronary artery disease
Nutrition and nutritional supplements in the management of dyslipidemia
Arrhythmias
Asthma
The allergic patient
Food allergy and intolerance
Integrative medicine and immune support
Prediabetes
Diabetes mellitus
Hypothyroidism
Polycystic ovarian syndrome
Osteoporosis
An integrative approach to obesity
MTHFR polymorphisms, homocysteine, and nutrient needs
Stress-induced hypothalamic-pituitary-adrenal axis dysfunction
Chronic kidney disease
Irritable bowel syndrome
Gastroesophageal reflux disease and functional dyspepsia
Peptic ulcer disease
Recurring abdominal pain in pediatrics
Constipation
Nonalcoholic steatohepatitis/fatty liver
Fibromyalgia
Chronic fatigue syndrome
Rheumatoid arthritis
Inflammatory bowel disease
Preconception counseling and fertility
Perimenopause to menopause
Premenstrual syndrome
Dysmenorrhea
Uterine fibroids (leiomyomata)
Vaginal dryness
Benign prostatic hyperplasia
Urolithiasis (kidney and bladders stones)
Chronic prostatitis
Erectile dysfunction
Testosterone deficiency
Osteoarthritis
Myofascial pain syndrome
Chronic low back pain
Neck pain
Gout
Carpal tunnel syndrome
Atopic dermatitis
Psoriasis
Urticaria
Hair loss
Acne vulgaris and acne rosacea
Breast cancer
Lung cancer
Prostate cancer
Colorectal cancer
Palliative and end of life care
Addiction treatment
Cataracts
Macular degeneration
The elimination diet
The glycemic index/load
The antiinflammatory diet
The DASH diet
The FODMaPs diet
Rethinking the movement prescription
Therapeutic breathing
Low back pain exercises
Relaxation techniques
Self-hypnosis techniques
Enhancing heart rate variability
Guided imagery and interactive guided imagery
Journaling for health
Forgiveness
Recommending meditation
Motivational interviewing techniques
Emotional awareness for pain
Compassion practices
Prescibing botanicals
Prescribing probiotics
Prescribing low-dose naltrexone
Detoxification
Chelation therapy
Psychedelic-assisted psychotherapy for health
Tapering off a proton pump inhibitor
Tapering off opioids
Integrative strategies for planetary health
Osteopathic manipulative treatment
Prolotherapy for chronic musculoskeletal pain
Integrating spiritual assessment and care
Biofield therapies
Integrative medicine for the underserved.Digital Access ProQuest Ebook Central 2023Limited to 3 simultaneous users