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- ArticleBellamy SE, Loor B, Gutierrez-Castillo M.Cureus. 2023 Jan;15(1):e34350.Cannabis is a commonly used illicit drug worldwide with many noted adverse effects and medical properties. It has been previously used in medicine for the management of chemotherapy-induced nausea and vomiting. The chronic use of cannabis is well-known to be associated with psychological and cognitive adverse effects, however, cannabinoid hyperemesis syndrome is a less commonly recognized complication of chronic long-term use of cannabis, though it does not occur in the majority of chronic cannabis users. Here we present the case of a 42-year-old male who presented with classical clinical features of cannabinoid hyperemesis syndrome.
- ArticleZamay GS, Zamay TN, Kolovskii VA, Shabanov AV, Glazyrin YE, Veprintsev DV, Krat AV, Zamay SS, Kolovskaya OS, Gargaun A, Sokolov AE, Modestov AA, Artyukhov IP, Chesnokov NV, Petrova MM, Berezovski MV, Zamay AS.Sci Rep. 2016 10 03;6:34350.The development of an aptamer-based electrochemical sensor for lung cancer detection is presented in this work. A highly specific DNA-aptamer, LC-18, selected to postoperative lung cancer tissues was immobilized onto a gold microelectrode and electrochemical measurements were performed in a solution containing the redox marker ferrocyanide/ferricyanide. The aptamer protein targets were harvested from blood plasma of lung cancer patients by using streptavidin paramagnetic beads and square wave voltammetry of the samples was performed at various concentrations. In order to enhance the sensitivity of the aptasensor, silica-coated iron oxide magnetic beads grafted with hydrophobic C8 and C4 alkyl groups were used in a sandwich detection approach. Addition of hydrophobic beads increased the detection limit by 100 times. The detection limit of the LC-18 aptasensor was enhanced by the beads to 0.023 ng/mL. The formation of the aptamer - protein - bead sandwich on the electrode surface was visualized by electron microcopy. As a result, the electrochemical aptasensor was able to detect cancer-related targets in crude blood plasma of lung cancer patients.
- ArticleDeng J, Liao X, Cao H.Medicine (Baltimore). 2023 Jul 21;102(29):e34350.RATIONALE: Hyperparathyroidism is caused by parathyroid tumors combined with gastroenteropancreatic tumors and pituitary tumors, which is common in patients with multiple endocrine neoplasia 1 syndrome (MEN-1). As its main pathogenic factor involves genetic mutations, it can cause a variety of different clinical symptoms. However, cases with negative genetic testing results and multiple nonfunctional malignant neuroendocrine tumors (NETs) with metastasis are relatively rare.
PATIENT CONCERNS: A 33-year-old man was admitted to the hospital for hyperparathyroidism. Imaging examination revealed multiple nodules in the parathyroid gland, pancreas, thymus, and adrenal gland, and multiple metastases to the lung, liver, thoracolumbar, as well as mediastinal lymph nodes.
DIAGNOSES: After multidisciplinary consultation, this patient was diagnosed with MEN-1 syndrome with various original tumors and multiple systemic metastases.
INTERVENTIONS: The patient underwent parathyroid tumor resection and metastasis biopsy.
OUTCOMES: The patient received denosumab and sorafenib treatment.
LESSONS: As an autosomal dominant hereditary disease, MEN-1 patients present with parathyroid hyperplasia, pancreatic and intestinal tumors, pituitary tumors, and so on, which are caused by genetic mutations. These patients would have hyperparathyroidism, hypoglycemia, gastric ulcer, and gastrointestinal diseases. However, some patients with MEN-1 syndrome cannot be diagnosed by genetic testing and simultaneously present with multiple nonfunctional NETs with systemic metastasis. This increases the difficulty of diagnosis and the subsequent treatment. - ArticleKragt A, Voorn-Brouwer T, van den Berg M, Distel B.J Biol Chem. 2005 Oct 07;280(40):34350-7.Recent studies on the sorting of peroxisomal membrane proteins challenge the long-standing model in which peroxisomes are considered to be autonomous organelles that multiply by growth and division. Here, we present data lending support to the idea that the endoplasmic reticulum (ER) is involved in sorting of the peroxisomal membrane protein Pex3p, a protein required early in peroxisome biogenesis. First, we show that the introduction of an artificial glycosylation site into the N terminus of Pex3p leads to partial N-linked core glycosylation, indicative of insertion into the ER membrane. Second, when FLAG-tagged Pex3p is equipped with an ER targeting signal, it can restore peroxisome formation in pex3Delta cells. Importantly, FLAG antibodies that specifically recognize the processed Pex3p show that the signal peptide of the fusion protein is efficiently cleaved off and that the processed protein localizes to peroxisomes. In contrast, a Pex3p construct in which cleavage of the signal peptide is blocked by a mutation localizes to the ER and the cytosol and cannot complement pex3Delta cells. Together, these results strongly suggest that ER-targeted Pex3p indeed routes via the ER to peroxisomes, and we hypothesize that this pathway is also used by endogenous Pex3p.
- ArticleZhu Y, Han J, Zhang Q, Zhao Z, Wang J, Xu X, Hao H, Zhang J.RSC Adv. 2019 Oct 23;9(59):34350.[This corrects the article DOI: 10.1039/C9RA05650E.].
- ArticleKoutinas AA, Sypsas V, Kandylis P, Michelis A, Bekatorou A, Kourkoutas Y, Kordulis C, Lycourghiotis A, Banat IM, Nigam P, Marchant R, Giannouli M, Yianoulis P.PLoS One. 2012;7(4):e34350.Delignified cellulosic material has shown a significant promotional effect on the alcoholic fermentation as yeast immobilization support. However, its potential for further biotechnological development is unexploited. This study reports the characterization of this tubular/porous cellulosic material, which was done by SEM, porosimetry and X-ray powder diffractometry. The results showed that the structure of nano-tubular cellulose (NC) justifies its suitability for use in "cold pasteurization" processes and its promoting activity in bioprocessing (fermentation). The last was explained by a glucose pump theory. Also, it was demonstrated that crystallization of viscous invert sugar solutions during freeze drying could not be otherwise achieved unless NC was present. This effect as well as the feasibility of extremely low temperature fermentation are due to reduction of the activation energy, and have facilitated the development of technologies such as wine fermentations at home scale (in a domestic refrigerator). Moreover, NC may lead to new perspectives in research such as the development of new composites, templates for cylindrical nano-particles, etc.
- ArticleHaugh JM, Huang AC, Wiley HS, Wells A, Lauffenburger DA.J Biol Chem. 1999 Nov 26;274(48):34350-60.Regulated activation of the highly conserved Ras GTPase is a central event in the stimulation of cell proliferation, motility, and differentiation elicited by receptor tyrosine kinases, such as the epidermal growth factor receptor (EGFR). In fibroblasts, this involves formation and membrane localization of Shc.Grb2.Sos complexes, which increases the rate of Ras guanine nucleotide exchange. In order to control Ras-mediated cell responses, this activity is regulated by receptor down-regulation and a feedback loop involving the dual specificity kinase mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK). We investigated the role of EGFR endocytosis in the regulation of Ras activation. Of fundamental interest is whether activated receptors in endosomes can participate in the stimulation of Ras guanine nucleotide exchange, because the constitutive membrane localization of Ras may affect its compartmentalization. By exploiting the differences in postendocytic signaling of two EGFR ligands, epidermal growth factor and transforming growth factor-alpha, we found that activated EGFR located at the cell surface and in internal compartments contribute equally to the membrane recruitment and tyrosine phosphorylation of Shc in NR6 fibroblasts expressing wild-type EGFR. Importantly, both the rate of Ras-specific guanine nucleotide exchange and the level of Ras-GTP were depressed to near basal values on the time scale of receptor trafficking. Using the selective MEK inhibitor PD098059, we were able to block the feedback desensitization pathway and maintain activation of Ras. Under these conditions, the generation of Ras-GTP was not significantly affected by the subcellular location of activated EGFR. In conjunction with our previous analysis of the phospholipase C pathway in the same cell line, this suggests a selective continuation of specific signaling activities and cessation of others upon receptor endocytosis.
- ArticleYeh CN, Wu C, Su H, Chai JD.RSC Adv. 2018 Oct 04;8(60):34350-34358.To fully utilize the great potential of graphene in electronics, a comprehensive understanding of the electronic properties of finite-size graphene flakes is essential. While the coronene series with n fused benzene rings at each side (designated as n-coronenes) are possible structures for opening a band gap in graphene, their electronic properties are not yet fully understood. Nevertheless, because of their radical character, it remains very difficult to reliably predict the electronic properties of the larger n-coronenes with conventional computational approaches. In order to circumvent this, the various electronic properties of n-coronenes (n = 2-11) are investigated using thermally-assisted-occupation density functional theory (TAO-DFT) [J.-D. Chai, J. Chem. Phys., 2012, 136, 154104], a very efficient electronic structure method for studying nanoscale systems with strong static correlation effects. The ground states of the larger n-coronenes are shown to be polyradical singlets, where the active orbitals are mainly localized at the zigzag edges.
- ArticleWang X, Tao Z, Pan G, Zou H, Xie Y, Li X.Environ Sci Pollut Res Int. 2023 Mar;30(12):34350-34362.The transmission effect of industrial policies on the quality of innovation of micro-enterprises is a central concern that attracts current academics and policy makers. Using the 2004-2019 data of A-shares of listed companies in Shanghai and Shenzhen, as well as the policies issued by Chinese ministries and departments at the ministry level and above, this paper empirically investigates the impact and mechanism of industrial policies on the innovation quality of wind power companies. The research results demonstrate that policies all play a significant role in promoting the quality of enterprise innovation of wind power. The intermediary role of credit financing of enterprises between different types of industrial guidelines and the quality of corporate innovation is different. Executive equity incentives positively moderate the relationship between different types of industrial policies and corporate credit financing and effectively weaken the adverse effects of regulatory policies, and it can also significantly enhance the role of policies in promoting the quality of corporate innovation. Finally, this paper gives targeted policy recommendations for the development of new energy industry innovation in China and similar countries and regions from the perspective of government and enterprise.
- ArticleSong T, Su X, He J, Liang Y, Zhou T, Liu C.Environ Sci Pollut Res Int. 2018 Dec;25(34):34343-34350.In this study, we collected crop plants and associated soil samples and determined these for selenium (Se) content to analyze the uptake, enrichment, and translocation of Se in the different soil-plant systems of an agricultural production area, elucidate the dynamic mechanisms relating to Se content in plants and soil during different growth periods, and screen plants for high Se enrichment ability. Bioconcentration factor determinations indicated that the grains of rice have the strongest Se enrichment ability, followed by soybean and corn. Translocation factor analysis indicated that the grains of rice and corn have similar low translocation abilities for Se compared with soybean. Within the study area, the Se content in plants was closely related to the soil available Se content and varied considerably among different growth periods and plant organs. This study provides a theoretical basis for the development and utilization of local agricultural products.