ArticleAmi D, Bonecchi L, Calì S, Orsini G, Tonon G, Doglia SM.
Biochim Biophys Acta. 2003 Dec 05;1624(1-3):6-10.
Two recombinant Escherichia coli strains expressing different levels of an interferon fusion protein as inclusion bodies have been studied by Fourier transform infrared (FT-IR) microspectroscopy. A marker band at 1628 cm(-1) allowed monitoring of the protein expression by direct analysis of cell pellets in a rapid, non-invasive and quantitative way. The results demonstrate that FT-IR microspectroscopy is a technique of potential biotechnological interest for studying inclusion body formation.