BookJiye Cai, editor.
Summary: The book addresses new achievements in AFM instruments - e.g. higher speed and higher resolution - and how AFM is being combined with other new methods like NSOM, STED, STORM, PALM, and Raman. This book explores the latest advances in atomic force microscopy and related techniques in molecular and cell biology. Atomic force microscopy (AFM) can be used to detect the superstructures of the cell membrane, cell morphology, cell skeletons and their mechanical properties. Opening up new fields of in-situ dynamic study for living cells, enzymatic reactions, fibril growth and biomedical research, these combined techniques will yield valuable new insights into molecule and cell biology. This book offers a valuable resource for students and researchers in the fields of biochemistry, cell research and chemistry etc.
Contents:
Intro; Preface; Acknowledgments; Contents; List of Contributors; About the Editor; Chapter 1: Principles of Atomic Force Microscopy; 1 AFM Working Principles; 2 Contact Mode; 3 Tapping Mode; 4 PID Feedback Loop; 5 Force Mode; 6 Peak Force Tapping; 7 Molecular Recognition; 8 Frequency Modulation Mode; Reference; Chapter 2: Atomic Force Microscopy-Based Single Molecule Force Spectroscopy for Biological Application; 1 Introduction; 1.1 AFM (Atomic Force Microscopy); 1.2 The Importance of Biomolecular Interactions; 2 Force Spectroscopy of Proteins on Cell Surface 3 Protein AFM-SMFS with DNA Aptamer Tips4 AFM-SMFS of Cell Surface Glycans; 5 Perspective; References; Chapter 3: In Situ Single Molecule Detection on Cell Membrane and Label Molecule Distributions Using AFM/NSOM; 1 Introduction; 2 Functionalization of AFM Tips; 3 Basic Principle for AFM-SMFS; 4 Basic Principle for AFM-TREC; 5 Basic Principle of NSOM for In Situ Single Molecule Detection on Cell Membrane; 6 Mapping Cell Membrane Biomolecules by AFM-SMFS; 7 Probing Cell Membrane Biomolecules by AFM-TREC; 8 Application of NSOM for In Situ Single Molecule Detection on Cell Membrane 3.2 High Speed AFM with dSTORM3.3 High Speed AFM with PALM; 4 Hyphenated High Speed AFM and Super-Resolution Optical Techniques for Manipulation; 4.1 AFM Nanomanipulation; 4.2 Force Spectroscopy; 4.3 Fast Force Mapping; 4.4 AFM Manipulation
STED; 4.5 AFM Nano-Manipulation
Optical Spectroscopy; 4.6 AFM Manipulation
TIRF Microscopy; 5 Prospect and Outlook; 6 Conclusion; References; Chapter 7: AFM and NSOM/QD Based Direct Molecular Visualization at the Single-Cell Level; 1 AFM and NSOM/QD Based Nanoscale Imaging System 4.1 Real-Time Imaging of Living Cells4.2 Imaging of Organelles and Proteins; 4.3 Multiparametric Force Probing and Molecular Recognition; 5 Combining Advanced Optical Microscopy with AFM; 5.1 Combining CLSM with AFM; 5.2 Combining NSOM with AFM; 6 Conclusions; References; Chapter 6: The Hyphenated Technique of High Speed Atomic Force Microscopy and Super Resolution Optical Detection System; 1 Introduction; 2 High Speed AFM; 2.1 A High Bandwidth Z-Scanner; 2.2 A Small Cantilever; 3 Combination of High Speed AFM with Super-Resolution Optical Techniques for Imaging; 3.1 High Speed AFM with STED 9 Combining AFM and NSOM for In Situ Single Molecule Imaging on Cell Membrane10 Conclusions and Future Perspectives; References; Chapter 4: AFM Imaging-Force Spectroscopy Combination for Molecular Recognition at the Single-Cell Level; 1 Adhesion Force Mapping Using Force Spectroscopy; 2 Topographic and Recognition (TREC) Imaging; 3 Conclusions; References; Chapter 5: Atomic Force Microscopy: A Nanoscopic Application in Molecular and Cell Biology; 1 Introduction; 2 Basic Principles of AFM; 3 Submolecular Imaging of Cell Membranes; 4 Nanoscale Functional Imaging of Cells