BookJunjie Xiao, editor.
Summary: This book presents the latest research on non-coding RNAs in cardiovascular disease, a major cause of death worldwide. Non-coding RNAs play a significant role in development, proliferation, differentiation and apoptosis. Since altered non-coding RNA expression is often associated with various diseases, their potential use in diagnostics, prognostics and therapeutics is an important current area of study. The book consists of six parts: 1) An overview of non-coding RNAs and cardiovascular system, 2) Bioinformatics and interactions, 3) Non-coding RNA regulation in cardiovascular system, 4) Non-coding RNAs and cardiovascular diseases, 5) Potential biomarkers and therapeutic implications, 6) Future prospects. It is particularly useful for researchers and students in the field of non-coding RNA and cardiovascular biology, as well as for cardiologists, pharmacologists and physiologists.
Contents:
3 Analysis of ncRNA Functions by Computational Methods Intro
Contents
Part I: Overview
1: An Overview of Non-coding RNAs and Cardiovascular System
1 Background
1.1 Discovery of Non-coding RNAs
1.2 Types of Non-coding RNAs
1.3 Classification of Long Non-coding RNAs
1.3.1 Classification of Long Non-coding RNAs According to Structural Organization
1.3.2 Functional Classification of Long ncRNAs
2 Methods to Study Non-coding RNAs
3 Mechanisms of Non-coding RNAs Regulation
3.1 The Transcriptional Regulation Control
3.2 The Post-transcriptional Regulation Control
3.3 miRNA-Independent mRNA Degradation 3.4 Transient lncRNA Transcribed from Active Enhancers
3.5 eRNA as Enhancer in Calcium Signalling
4 LncRNA Genes with Enhancer-like Activity
5 Micro RNAs as a Source of Non-coding RNA
5.1 Micro RNA as Negative Regulator of Gene Expression
6 Circular RNAs (CircRNAs) as Non-coding RNAs
7 Non-Coding RNA Functions
7.1 Functions of Long Non-coding RNAs
7.2 Micro RNAs as Non-coding RNAs in Cardiovascular Diseases
7.3 Role of Long Non-coding RNA in Cardiovascular Diseases
7.4 Long Non-coding RNA in Myocardial Infarction and Atherosclerosis 7.5 Non-Coding RNAs in Apoptosis Linked Cardiovascular Pathologies
7.5.1 Micro RNAs as Regulator of Apoptosis
7.5.2 Long Non-coding RNAs in Apoptosis
8 miRNA as Therapeutic Target in Cardiovascular Diseases
8.1 Role in Acute Myocardial Infarction
8.2 Role in Fibrosis
8.3 Role of miRNA in Cardiac Hypertrophy and Failure
8.4 Role of miRNA in Atherosclerosis and Remodeling
8.5 miRNA as a Therapeutic Approach for CVDs
9 miRNA Modification
9.1 Antisense Oligonucleotides
9.2 siRNAs
9.3 Restoration of miRNA Levels 9.4 Therapeutic Potential of AntagomiRs
9.5 Therapeutic Potential of LNA AntimiRs
9.6 Challenges for AntimiR
10 miRNAs as Biomarker
10.1 miRNA as Biomarker in Atherosclerosis
10.2 miRNAs as Biomarker in Ischemic Stroke
10.3 miRNAs as Biomarker in Hypertension
10.4 miRNAs as Biomarker in Hypertrophy and Heart Failure
11 The Role of Long Non-coding RNA in Cardiovascular System
11.1 Role of lncRNA in Myocardial Infarction
11.2 Role of lncRNA in Cardiac Fibrosis and Hypertrophy
11.3 Role of lncRNA in Atherosclerosis and Angiogenesis 12 Targeting lncRNA for the Treatment of Cardiovascular Diseases
12.1 Restoration of lncRNA by Overexpression
12.2 Circulating LnRNA as Biomarkers for Cardiovascular Diseases
12.3 Challenges for lncRNA Therapeutics
References
Part II: Bioinformatics and Interactions
2: Bioinformatics Research Methodology of Non-coding RNAs in Cardiovascular Diseases
1 Introduction
2 Wet-Lab Methodological Considerations
2.1 Sample Preparation
2.2 Non-coding RNA Quantification
2.3 Data Processing from Quantification Methods