Bookedited by Bettina Warscheid.
Contents:
1. Fifteen Years of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) / Matthias Mann
2. Stable Isotope Labeling by Amino Acids Applied to Bacterial Cell Culture / Boumediene Soufi and Boris Macek
3. SILAC Labeling of Yeast for the Study of Membrane Protein Complexes / Silke Oeljeklaus, Andreas Schummer, Ida Suppanz, and Bettina Warscheid
4. Whole Proteome Analysis of the Protozoan Parasite Trypanosoma brucei Using Stable Isotope Labeling by Amino Acids in Cell Culture and Mass Spectrometry / Olivera Cirovic and Torsten Ochsenreiter
5. Stable Isotope Labeling by Amino Acids in Cultured Primary Neurons / Guoan Zhang, Katrin Deinhardt, and Thomas A. Neubert
6. SILAC and Alternatives in Studying Cellular Proteomes of Plants / Annemarie Matthes, Karin Köhl, and Waltraud X. Schulze
7. In Vivo Stable Isotope Labeling by Amino Acids in Drosophila melanogaster / Matthias D. Sury, Jia-Xuan Chen, and Matthias Selbach
8. Stable Isotope Labeling for Proteomic Analysis of Tissues in Mouse / Soraya Hölper, Aaron Ruhs, and Marcus Krüger
9. Identification of Novel Protein Functions and Signaling Mechanisms by Genetics and Quantitative Phosphoproteomics in Caenorhabditis elegans / Julius Fredens, Kasper Engholm-Keller, Jakob Møller-Jensen, Martin Røssel Larsen, and Nils J. Færgeman
10. SILAC-Based Temporal Phosphoproteomics / Chiara Francavilla, Omid Hekmat, Blagoy Blagoev, and Jesper V. Olsen
11. Global Ubiquitination Analysis by SILAC in Mammalian Cells / Zhiping Wu, Chan Hyun Na, Haiyan Tan, and Junmin Peng
12. Quantifying In Vivo, Site-Specific Changes in Protein Methylation with SILAC / Ho-Tak Lau, Karen A. Lewis, and Shao-En Ong
13. Applying SILAC for the Differential Analysis of Protein Complexes / Karsten Boldt, Christian J. Gloeckner, Yves Texier, Felix von Zweydorf, and Marius Ueffing
14. Defining Dynamic Protein Interactions Using SILAC-Based Quantitative Mass Spectrometry / Xiaorong Wang and Lan Huang
15. Identifying Nuclear Protein-Protein Interactions Using GFP Affinity Purification and SILAC-Based Quantitative Mass Spectrometry / H. Irem Baymaz, Cornelia G. Spruijt, and Michiel Vermeulen
16. Analyzing the Protein Assembly and Dynamics of the Human Spliceosome with SILAC / Carla Schmidt, Monika Raabe, Reinhard Lührmann, and Henning Urlaub
17. Identification and Validation of Protein-Protein Interactions by Combining Co-immunoprecipitation, Antigen Competition, and Stable Isotope Labeling / Frederik Sommer, Timo Mühlhaus, Dorothea Hemme, Daniel Veyel, and Michael Schroda
18. Protein Correlation Profiling-SILAC to Study Protein- Protein Interactions / Anders R. Kristensen and Leonard J. Foster
19. Autophagosomal Proteome Analysis by Protein Correlation Profiling-SILAC / Andrea C. Becker and Jörn Dengjel
20. Design and Application of Super-SILAC for Proteome Quantification / Yair Pozniak and Tamar Geiger
21. Proteomics Meets Genetics: SILAC Labeling of Drosophila melanogaster Larvae and Cells for In Vivo Functional Studies / Alessandro Cuomo, Roberta Sanfilippo, Thomas Vaccari, and Tiziana Bonaldi
22. Analysis of Secreted Proteins Using SILAC / Jeanette Henningsen, Blagoy Blagoev, and Irina Kratchmarova
23. Identification of MicroRNA Targets by Pulsed SILAC / Markus Kaller, Silke Oeljeklaus, Bettina Warscheid, and Heiko Hermeking
24. MaxQuant for In-Depth Analysis of Large SILAC Datasets / Stefka Tyanova, Matthias Mann, and Jürgen Cox.