Bookedited by Yu Wai Chen.
Contents:
1. DisMeta : a meta server for construct design and optimization / Yuanpeng Janet Huang, Thomas B. Acton, and Gaetano T. Montelione
2. Stable expression clones and auto-induction for protein production in E. coli / F. William Studier
3. High-throughput expression screening and purification of recombinant proteins in E. coli / Natalie J. Saez and Renaud Vincentelli
4. Medium-throughput production of recombinant human proteins : ligation-independent cloning / Claire Strain-Damerell, Pravin Mahajam, Opher Gileadi and Nicola A. Burgess-Brown
5. Medium-throughput production of recombinant human proteins : protein production in E. coli / Nicola A. Burgess-Brown, Pravin Mahajan, Claire Strain-Damerell, Opher Gileadi and Susanne Gräslund
6. Medium-throughput production of recombinant human proteins : protein production in insect cells / Pravin Mahajan , Claire Strain-Damerell, Opher Gileadi and Nicola A. Burgess-Brown
7. OmniBac : universal multigene transfer plasmids for baculovirus expression vectors systems / Deepak B. Thimiri Govinda Raj, Lakshmi S. Vijayachandran, and Imre Berger
8. Multiprotein complex production in insect cells by using polyproteins / Yan Nie, Itxaso Bellon-Echeverria, Simon Trowitzch, Christoph Bieniossek and Imre Berger
9. Expression screening in mammalian suspension cells / Susan D. Chapple and Michael R. Dyson
10. Cell-free expression of protein complexes for structural biology / Takaho Terada, Takehi Murata, Mikako Shirouzu and Shigeyuki Yokoyama
11. Cell-free protein synthesis for functional and structural studies / Shin-ichi Makino, Emily T. Beebe, John L. Markley and Brian G. Fox
13. Insoluble protein purification with sarkosyl : facts and precautions / Ben Chisnall, Courtney Johnson, Yavuz Kulaberoglu and Yu Wai Chen
13. Estimation of crystallization likelihood through a fluorimetric thermal stability assay / Vincent Mariaule, Florine Dupeux, and José A. Márquez
14. CrystalDirect : a novel approach for automated crystal harvesting based on photoablation of thin films / José A. Márquez and Florent Cipriani
15. Methods to refine macromolecular structures in cases of severe diffraction anisotropy / Michael R. Sawaya
16. Applications of NMR-based PRE and EPR-based DEER spectroscopy to homodimer chain exchange characterization and structure determination / Yunhuang Yang, Theresa A. Ramelot, Shuisong Ni, Robert M. McCarrick and Michael A. Kennedy
17. Cost-effective protocol for the parallel production of libraries of 13CH3-specifically labeled mutants for NMR studies of high molecular weight proteins / Elodie Crublet, Rime Kerfah, Guillaume Mas, Marjolaine Noirclerc-Savoye, Violaine Lantez, Thierry Vernet and Jerome Boisbouvier
18. High-throughput SAXS for the characterization of biomolecules in solution : a practical approach / Kevin N. Dyer, Michael Hammel, Robert P. Rambo, Susan E. Tsutakawa, Ivan Rodic, Scott Classen, John A. Tainer and Greg L. Hura
19. Measuring spatial restraints on native protein complexes using isotope-tagged chemical cross-linking and mass spectrometry / Franz Herzog
20. Modeling of proteins and their assemblies with the integrative modeling platform / Benjamin Webb, Keren Lasker, Javier Velázquez-Muriel, Dina Schneidman-Duhovny, Riccardo Pellarin, Massimiliano Bonomi, Charles Greenberg, Barak Raveh, Elina Tjioe, Daniel Russel and Andrej Sali
21. Quality and validation of structures from structural genomics / Marcin J. Domagalski, Heping Zheng, Matthew D. Zimmerman, Zhigniew Dauter, Alexander Wlodawer and Wladek Minor
22. Navigating the global protein-protein interaction landscape using iRefWeb / Andrei L. Turinsky, Sabry Razick, Brian Turner, Ian M. Donaldson and Shoshana J. Wodak
23. Mespeus-a database of metal interactions with proteins / Marjorie M. Harding and Kun-Yi Hsin
24. High-quality macromolecular graphics on mobile devices : a quick starter's guide / Chin-Pang Benny Yiu and Yu Wai Chen.