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- ArticleBarber PC, Raisman G.Brain Res. 1978 Aug 25;152(2):303-11.Application of [3H]proline to the vomeronasal organ (VNO) in mice results in the transport of labelled material along the vomeronasal axons to their terminals in the glomerular layer of the accessory olfactory bulb (AOB). In addition labelled material leaves the vomeronasal nerve terminals and is found over the external plexiform layer (EPL), where a previous electron microscopic autoradiographic study showed that it is preferentially accumulated in mitral cells. Grain densities over the glomerular layer and the EPL were counted in light micrographs. After subtracting background, the overall density of grains in the EPL is about 10% of that over the glomerular layer at 6 h after administration of [3H]proline to the VNO (5 mice). In a further 7 mice, puromycin (or saline) was applied directly to the AOB at hourly intervals during the 6 h after [3H]proline administration. Under these circumstances the labelling in the EPL is only 2--4% of that in the glomerular layer (9% for the 2 saline controls). These observations are evidence that a major part of the transsynaptic transfer mechanism is dependent on protein synthesis, and also favour the view that free amino acids are an important component of the material transferred.