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  • Article
    Chen G, Xie X.
    PLoS One. 2020;15(8):e0236709.
    BACKGROUND: With the development of second-generation sequencing technology, more and more DNA sequence variations have been detected. Exon sequencing is the first choice for sequencing many cancer genes, and it can be better used to identify disease status by detecting gene variants. PCR sequence is an effective method to capture that sequence of an exon in the process of sequencing. Exon sequencing sequence contains PCR primer sequence, the correct position of the sequence can be determined by PCR primer sequence, which can be found in SNP, Indel mutation point by comparing the sequence of PCR primer sequence.
    RESULTS: In this paper, a matching algorithm based on the PCR primer sequence is proposed, which can effectively sequence the position of PCR primer sequence and find out the key position sequence. Then the sequencing sequence is sorted and the number of the same sequence is counted to reduce the matching times. Then, the sequenced sequence was matched with PCR primer sequence, so that the DNA position could be accurately matched and the variation in the sequenced sequence could be found more quickly.
    CONCLUSIONS: Compared with the traditional sequence matching method, PCR primer sequence matching method can match many sequences and find more variation. It also showed a high recall rate in the recall rate.
    Digital Access Access Options
  • Book
    Richard T. McKeon, former Clinical Division Director, American Association of Suicidology.
    Summary: "A new edition with the latest approaches to assessment and treatment of suicidal behavior With more than 800,000 deaths worldwide each year, suicide is one of the leading causes of death. The second edition of this volume incorporates the latest research, showing which empirically supported approaches to assessment, management, and treatment really help those at risk. Updates include comprehensively updated epidemiological data, the role opioid use problems, personality disorders, and trauma play in suicide, new models explaining the development of suicidal ideation, and the zero suicide model. This book aims to increase clinicians' access to empirically supported interventions for suicidal behavior, with the hope that these methods will become the standard in clinical practice. The book is invaluable as a compact how-to reference for clinicians in their daily work and as an educational resource for students and for practice-oriented continuing education. Its reader-friendly structure makes liberal use of tables, boxed clinical examples, and clinical vignettes. The book, which also addresses common obstacles in treating individuals at risk for suicide, is an essential resource for anyone working with this high-risk population."-- Provided by publisher.

    Contents:
    1 Description
    2 Theories and Models of Suicidal Behavior
    3 Risk Assessment and Treatment Planning
    4 Treatment
    5 Case Vignette
    6 Case Example
    7 Further Reading
    8 References
    9 Appendix: Tools and Resources
    Digital Access Ovid 2021