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  • Article
    Perello C, Llamas E, Burlat V, Ortiz-Alcaide M, Phillips MA, Pulido P, Rodriguez-Concepcion M.
    PLoS One. 2016;11(2):e0150539.
    Plastidial isoprenoids are a diverse group of metabolites with roles in photosynthesis, growth regulation, and interaction with the environment. The methylerythritol 4-phosphate (MEP) pathway produces the metabolic precursors of all types of plastidial isoprenoids. Proteomics studies in Arabidopsis thaliana have shown that all the enzymes of the MEP pathway are localized in the plastid stroma. However, immunoblot analysis of chloroplast subfractions showed that the first two enzymes of the pathway, deoxyxylulose 5-phosphate synthase (DXS) and reductoisomerase (DXR), can also be found in non-stromal fractions. Both transient and stable expression of GFP-tagged DXS and DXR proteins confirmed the presence of the fusion proteins in distinct subplastidial compartments. In particular, DXR-GFP was found to accumulate in relatively large vesicles that could eventually be released from chloroplasts, presumably to be degraded by an autophagy-independent process. Together, we propose that protein-specific mechanisms control the localization and turnover of the first two enzymes of the MEP pathway in Arabidopsis chloroplasts.
    Digital Access Access Options
  • Article
    Ewbank AC, Fuentes-Castillo D, Sacristán C, Cardoso B, Esposito F, Fuga B, de Macedo EC, Lincopan N, Catão-Dias JL.
    Sci Total Environ. 2022 Feb 01;806(Pt 2):150539.
    Antimicrobial resistance is among the most serious public health threats of the 21st century, with great impact in terms of One Health. Among antimicrobial resistant bacteria (ARB), extended-spectrum ß-lactamase (ESBL)-producing Escherichia coli (ESBL-EC) represent major challenges to human healthcare. Wild birds have been commonly used as environmental bioindicators of ESBL-EC. Remote locations represent a unique opportunity to evaluate the occurrence, dissemination and epidemiology of ARB in the environment. Herein we surveyed ESBL-EC in 204 cloacal swabs from six nonsynanthropic seabird species at the pristine Rocas Atoll, Brazil. We identified ESBL-EC isolates in 2.4% (5/204) of the tested seabirds, all in magnificent frigatebirds (Fregata magnificens). We isolated strains of O25b-ST131-fimH22 harboring gene blaCTX-M-8 (3 clones), ST117 harboring gene blaSHV-12, and a novel ST11350 (clonal complex 349) harboring genes blaCTX-M-55 and fosA3. All the isolates presented Extraintestinal pathogenic E. coli (ExPEC) virulence profiles. We suggest that magnificent frigatebirds may act as "flying bridges", transporting ESBL-EC and ARGs from an anthropogenically-impacted archipelago geographically close to our pristine and remote study site. The characteristics of our isolates suggest zoonotic potential and, despite the apparent good health of all the evaluated birds, may represent a hypothetical potential threat to the avian population using the atoll. To our knowledge, this is the first description of: (1) the pandemic and public health relevant ST131-O25b harboring blaCTX-M-8 worldwide; (2) ST131-fimH22 in wild birds; and (3); fosA3 in wildlife. Our findings expand the current epidemiological knowledge regarding host and geographical distribution of ESBL-EC and ARGs in wild birds, and emphasize the disseminating characteristics and adaptability of ST131 and ST117 strains within the human-animal-interface. Herein we discuss the involvement of nonsynanthropic wild birds in the epidemiology of antimicrobial resistance and their potential as sentinels of ESBL E. coli in insular environments.
    Digital Access Access Options
  • Book
    [edited by] John Lorenz, Michel G. Baum, Kathleen G. Brennan ; consulting editor, Richard A. Polin.
    Summary: "Dr. Richard Polin's Neonatology Questions and Controversies series highlights the toughest challenges facing physicians and care providers in clinical practice, offering trustworthy guidance on up-to-date diagnostic and treatment options in the field. In each volume, renowned experts address the clinical problems of greatest concern to today's practitioners, helping you handle difficult practice issues and provide optimal, evidence-based care to every patient."--Provided by publisher.

    Contents:
    Assessment of Renal Function in Fetuses With Lower Urinary Tract Obstruction
    Assessment of Neonatal Kidney Function
    Diagnosis and Management of Neonatal Acute Kidney Injury
    Pulmonary Hypoplasia in the Fetus With Oligohydramnios: Causes, Treatment,and Prevention
    Pathophysiology and Management of Hyperkalemia in the Neonate
    Physiological Management of Fluid and Electrolyte Therapy in Newborns
    Pathophysiology and Management of Hyponatremia and Hypernatremia in the Neonate
    Genetic Causes of Congenital Anomalies of the Kidney and Urinary Tract
    Evidence-Based Approach to Diuretic Therapy in the Neonate
    Effect of Preterm Birth on Renal Outcomes
    Diagnosis and Management of Prenatal Urinary Tract Dilatation
    Dialysis and Kidney Transplantation in Infancy
    Renal Tubular Acidosis
    Renal Near-Infrared Spectroscopy Use in the Neonate
    Urosepsis and Uroprophylaxis
    Neonatal Hypertension.
    Digital Access ClinicalKey [2024]
doi:10.1371/journal.pone.0150539 doi:10.1016/j.scitotenv.2021.150539