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- ArticleZhang Z, Yu X, Zhao J, Shi X, Sun A, Jiao H, Xiao T, Li D, Chen J.Chemosphere. 2020 May;246:125622.Molecularly imprinted polymers (MIPs) are attracting substantial interest as artificial plastic antibodies because of their biometric capability for targeting small molecules. In this study, molecularly imprinted silica material-coated quantum dots (MIS-QDs) with selective recognition capability to okadaic acid (OA) were developed and characterized. The synthesized MIS-QDs with specific imprinting cavities exhibited excellent recognition capability similar to those of biological antibodies and high fluorescence (FL) quenching selectivity for OA. Furthermore, the MIS-QDs with unsaturated bonds were immobilized onto the surface of 96-well microplates by cold plasma-induced grafting. A novel direct competitive microplate assay strategy was then proposed. The FL quenching properties of the developed microplate assay showed an excellent linear relationship with OA in the range of 10.0-100.0 μg/kg with a correlation coefficient of 0.9961. The limit of detection for OA was 0.25 μg/kg in the shellfish samples. The mean quantitative recoveries were 92.5%-101.0% and 92.9%-101.3%, with relative standard deviations of <7.7% and 7.6% for pure solvents and purified shellfish samples, respectively. The established microplate assay strategy can be used as a rapid and high-throughput method for analyzing OA marine toxins in biological samples.
- ArticleWu Y, Liang R, Chen W, Wang C, Xing D.Talanta. 2024 Apr 01;270:125622.Alkaline phosphatase (ALP) is a zinc-containing metalloprotein that shows very great significance in clinical diagnosis, which can catalyze the hydrolysis of phosphorylated species. ALP has the potential to serve as a valuable biomarker for detecting liver dysfunction and bone diseases. On the other hand, ALP is an efficient biocatalyst to amplify detection signals in the enzyme-linked assay. It has always been a major research focus to develop novel biosensors that can detect ALP activity with high selectivity and sensitivity. There have been numerous reports on the development of biosensors to determine ALP activity using a phosphorylated DNA probe. Among them, various beneficial strategies, such as λ exonuclease-mediated cleavage reaction, terminal deoxynucleotidyl transferase-triggered DNA polymerization, and Klenow fragment polymerase-catalyzed elongation, are employed to generate amplified and more intuitive signal. This review discusses and summarizes the development and advances of biosensors for ALP activity detection that use a well-designed phosphorylated DNA probe, aiming to provide some guidelines for the design of more sophisticated sensing strategies that exhibit improved sensitivity, selectivity, and adaptability in detecting ALP activity.
- ArticleJang SS, Noh JY, Kim MC, Lim HA, Song MS, Kim HK.Microbiol Spectr. 2022 08 31;10(4):e0125622.Shaan virus (ShaV), a novel species of the genus Jeilongvirus, family Paramyxoviridae, was isolated from an insectivore bat (Miniopterus schreibersii) in Korea in 2016. ShaV particles contain a hemagglutinin-neuraminidase (HN) glycoprotein in their envelope that allows the virus to target cells. Typically, diverse paramyxoviruses with HN glycoprotein are reported to interact predominantly with sialic acids, but there are no studies of receptors for ShaV. In this study, the identification of potential receptors for ShaV was demonstrated using sialidase treatments, glycan microarray, magnetic bead-based virus binding assay, and neuraminidase inhibitor treatments. Pretreatment of MARC-145 cells with sialidase, which cleaves α2,3-linked sialic acids, showed higher inhibition of viral infection than α2,6-linked-specific sialidase. These data were supported by the binding of ShaV to predominantly α2,3-linked sialylated glycans in the screening of sialyl linkage patterns through glycan microarray. To further confirm the direct interaction between ShaV and α2,3-linked sialic acids, ShaV was incubated with α2,3- or α2,6-linked sialylated glycans conjugated to magnetic beads, and binding signals were detected only for α2,3-linked sialylated glycans. In addition, the potential of sialic acids as a receptor was demonstrated by the viral replication inhibitory effect of the neuraminidase inhibitor 2,3-dehydro-2-deoxy-N-acetylneuraminicacid (DANA) in the mature virion release steps. Collectively, these results support that α2,3-linked sialic acids are the potential receptor for ShaV infection in MARC-145 cells. IMPORTANCE Bats host major mammalian paramyxoviruses, and novel paramyxoviruses are increasingly being reported around the world. Shaan virus (ShaV), from the genus Jeilongvirus, family Paramyxoviridae, has a potential attachment glycoprotein, HN. Here, we identify that ShaV binds to sialic acid and demonstrate that α2,3-linked sialic acids are the potential receptor for ShaV infection. The presented data regarding ShaV receptor specificity will enable studies into the viral tropism for the host and contribute to the development of new antiviral strategies targeting viral receptors.
- ArticleBhat ZF, Morton JD, Mason SL, Bekhit AEA.Food Chem. 2020 Feb 15;306:125622.This study investigated the potential of pulsed electric field (PEF) as a sodium-reduction strategy for processed meat. Beef jerky was used as a model and prepared using different levels of NaCl, viz. 2.0% (control), 1.2% (T1) and 1.2% along with PEF processing (T2). A significant (p < 0.05) effect of PEF was observed on shear force (N) and toughness (N/mm s) of the products, which was also reflected in sensory scores. No effects for PEF were observed on colour, yield (%) and oxidative and microbial stability. PEF-treated samples (T2) had significantly (p < 0.05) lower sodium content than the control, however, the sensory scores were comparable (p > 0.05) with control and >84% of the panellists preferred T2 samples over T1 for saltiness. Results suggest that PEF treatment improved saltiness by influencing the salt diffusion and sodium delivery that led to better perception during chewing. PEF could be a novel method to produce healthier reduced-sodium meat products.
- ArticleCieciura-Włoch W, Borowski S, Domański J.Bioresour Technol. 2021 Nov;340:125622.The effect of nitrogen and phosphorous addition on hydrogen production from hydrolyzed Sugar beet pulp (SBP) was investigated using (NH4)3PO4, NH4Cl and K3PO4 as the supplements. In batch tests, the maximal hydrogen production of 279 dm3/kgVS was observed for K3PO4, which was added to SBP in a dose of 1 g/dm3. In semi-continuous experiments, the greatest hydrogen production of 36 dm3/kgVS was reported for the same supplement, and this value was twice higher than that of the control run. The analysis of microbiota revealed that the majority of bacteria was affiliated to the orders Clostridiales, Lactobacillales and Coriobacteriales. Moreover, a noticeable methane production was associated with the activity of Methanosphaera sp., which could grow in a low pH environment of dark fermentation.
- ArticleMartínez V, Lee D, Alyami I, Zimila H, Bautista F, Fuentes A, López MJ, Valencia G, Quanrud D, Arnold RG, Sáez AE.Environ Pollut. 2025 Feb 15;367:125622.Despite their potential risks to human health and the environment at ng/L to μg/L concentrations, there has been relatively little effort to measure trace organic compounds (TOrCs) in surface waters of Central America. The concentrations of eighteen TOrCs detected at eleven surface water sites in the Lempa River basin of El Salvador and four sources of drinking water for the cities of San Salvador, Antiguo Cuscatlán, Soyapango, and Santa Tecla are reported here. All samples were analyzed via liquid chromatography with tandem mass spectrometry (LC-MS/MS). Detected TOrCs in surface water included sixteen compounds. Maximum concentrations of 23 μg/L, 6 μg/L, and 2 μg/L were measured for sulfamethoxazole, sucralose, and bisphenol A, respectively. In tap water, a total of fourteen species were found, with peak concentrations of 17 μg/L for sulfamethoxazole, 640 ng/L for bisphenol A, and 224 ng/L for tris(chloropropyl) phosphate (TCPP). To assess potential mechanisms of TOrCs attenuation in surface waters, samples were irradiated with UVA light (315-400 nm) for 12 h in the presence of furfuryl alcohol (FFA) to establish singlet oxygen (1O2) formation. All the samples exhibited photosensitizing activity upon irradiation, resulting in 1O2 concentrations of the order of 10-14 M. To our knowledge, this is the first study that reports the quantification of TOrCs presence and the possible natural attenuation routes in Salvadoran surface and tap water sources.
- ArticleUphoff CC, Lange S, Denkmann SA, Garritsen HS, Drexler HG.PLoS One. 2015;10(4):e0125622.Contaminations of cell cultures with microbiological organisms are well documented and can be managed in cell culture laboratories applying reliable detection, elimination and prevention strategies. However, the presence of viral contaminations in cell cultures is still a matter of debate and cannot be determined with general detection methods. In the present study we screened 577 human cell lines for the presence of murine leukemia viruses (MLV). Nineteen cell lines were found to be contaminated with MLV, including 22RV1 which is contaminated with the xenotropic murine leukemia virus-related virus variant of MLV. Of these, 17 cell lines were shown to produce active retroviruses determined by product enhanced reverse transcriptase PCR assay for reverse transcriptase activity. The contaminated cell lines derive from various solid tumor types as well as from leukemia and lymphoma types. A contamination of primary human cells from healthy volunteers could not be substantiated. Sequence analyses of 17 MLV PCR products and five complete MLV genomes of different infected cell lines revealed at least three groups of related MLV genotypes. The viruses harvested from the supernatants of infected cell cultures were infectious to uninfected cell cultures. In the course of the study we found that contamination of human genomic DNA preparations with murine DNA can lead to false-positive results. Presumably, xenotransplantations of the human tumor cells into immune-deficient mice to determine the tumorigenicity of the cells are mainly responsible for the MLV contaminations. Furthermore, the use of murine feeder layer cells during the establishment of human cell lines and a cross-contamination with MLV from infected cultures might be sources of infection. A screening of cell cultures for MLV contamination is recommended given a contamination rate of 3.3%.
- ArticlePlaunt AJ, Rose SJ, Kang JY, Chen KJ, LaSala D, Heckler RP, Dorfman A, Smith BT, Chun D, Viramontes V, Macaluso A, Li Z, Zhou Y, Mark L, Basso J, Leifer FG, Corboz MR, Chapman RW, Cipolla D, Perkins WR, Malinin VS, Konicek DM.Antimicrob Agents Chemother. 2022 Nov 15;66(11):e0125622.
- ArticleZhu H, Chen J, Zhang Y, Goh KL, Wan C, Zheng D, Zheng M.Int J Biol Macromol. 2023 Aug 15;246:125622.Enzymatic hydrolysis is a promising approach for protein and food processing. However, the efficiency of this approach is constrained by the self-hydrolysis, self-agglomeration of free enzymes and the limited applicability resulted from enzymes' selectivityt. Here, novel organic-inorganic hybrid nanoflowers (AY-10@AXH-HNFs) were prepared by coordinating Cu2+ with both endopeptidase of PROTIN SD-AY10 and exopeptidase of Prote AXH. The results indicate that the AY-10@AXH-HNFs exhibited 4.1 and 9.6 times higher catalytic activity than free Prote AXH and PROTIN SD-AY10, respectively, for the enzymatic hydrolysis of N-benzoyl-L-arginine ethyl ester (BAEE). The kinetic parameters of Km, Vmax and Kcat/Km by AY-10@AXH-HNFs were determined to be 0.6 mg/mL, 6.8 mL·min/mg and 6.1 mL/(min·mg), respectively, surpassing the values obtained from free endopeptidase and exopeptidase. Furthermore, the ability of AY-10@AXH-HNFs to retain 41 % of their initial catalytic activity after undergoing 5 cycles of repeated use confirmed their stability and reusability. This study introduces a novel approach of co-immobilizing endopeptidase and exopeptidase on nanoflowers, resulting in significantly enhanced stability and reusability of the protease in catalytic applications.
- ArticleLi C, An S, Sun Q, Zhang X, Xu H, Yan B.Spectrochim Acta A Mol Biomol Spectrosc. 2025 Apr 05;330:125622.The electronic states of monochlorogermene (HGeCl) are computed by using the explicitly correlated internally contracted multireference configuration interaction (icMRCI-F12) method with Davidson correction. A total of 23 spin-free states with energy up to 9.4 eV as well as 55 spin-orbit coupled states generated by these spin-free states after considering the spin-orbit coupling (SOC) effect are investigated. The geometries and harmonic vibrational frequencies of the lowest electronic states are calculated, and the spectroscopic constants, oscillator strength, vertical transition energy and potential energy curves (PECs) of the spin-free states and spin-coupled states are presented. Based on the calculation of the electronic states, the photodissociation dynamics in the ultraviolet region is discussed. Our studies will shed light on the electronic structure and decomposition processes of HGeCl radicals.
- Bookedited by Renée Fleming.Summary: "World-renowned soprano and arts/health advocate Renee Fleming curates a collection of essays from leading scientists, creative arts therapists, educators, healthcare providers and artists about the powerful impacts of music and the arts on health and the human experience A compelling and growing body of research has shown music and arts therapies to be effective tools for addressing a widening array of conditions, from providing pain relief, to enhancing speech recovery after stroke or traumatic brain injury through singing, to improving mobility of individuals with Parkinson's disease using rhythm. In Music and Mind Renee Fleming draws upon her own experience as an advocate to showcase the breadth of this booming field, inviting leading experts to share their discoveries. In addition to describing therapeutic benefits, the book explores evolution, brain function, childhood development, and technology as applied to arts and health. Much of this area of study is relatively new, made possible by recent advances in brain imaging, and supported by the National Institutes of Health, major hospitals, and universities. This work is sparking an explosion of public interest in the arts and health sector. Fleming has presented on this material in over fifty cities across North America, Europe, and Asia, collaborating with leading researchers, policy-makers, and practitioners. With essays from known musicians, writers, and artists, as well as leading neuroscientists, Music and Mind is a groundbreaking book and the perfect introduction and overview of this exciting new field"-- Provided by publisher.
Contents:
Foreword / Francis Collins
Introduction / Renée Fleming
How and Why : Experts explain the basic science connecting arts and health, including origins in evolution. Musicality, Evolution, and Animal Responses to Music / Ani Patel ; What Does It Mean to Be Musical / Dan Levitin ; The Parting Glass / Richard Powers ; Sound Connects Us / Nina Kraus ; The Importance of Music in Human Connection / Rhiannon Giddens
The Medical Muse / Health professionals and artists share impacts of the arts in hospitals and clinical settings. Sounding Joy / Christopher Bailey ; Can Singing Touch the Heart? / Jacquelyn Kulinski ; Rabbit Hole / Rosanne Cash ; Music Therapy for Chronic Pain Management / Joke Bradt ; Music Across the Continuum of Care: A Medical Setting / J. Todd Frazier ; "I Sing the Body Electric": Music Psychotherapy in Medicine / Joanne Loewy
Health in the Spotlight : Artists and leading presenters discuss key health and community initiatives. How to Fall in Love with Opera / Ann Patchett ; Arts for Non-Arts Outcomes / Deborah Rutter ; Our Symphony Orchestra : the People's Band and a Symbol of Civilization / Ben Folds ; What Can Music Do? / Sarah Johnson ; Sing, Dance, and Play! / Mark Morris ; Dance and Parkinson's Finding Humanity Through a Musical Roadmap / David Leventhal
Creative Learning / Educators and researchers illuminate the effects of arts engagement on the developing brain. Humans Are Musical Creatures : The Case for Music Education / Indre Viskontas ; State of the Art: Music in America's Schools / Ken Elpus ; Rhythm & Rhyme: Supporting Young Children and Families with Musical Play / Miriam Lense and Sara Beck ; Healing Arts / Anna Deavere Smith ; Play on Philly / Stanford Thompson ; Building Belonging Through Music : The Art of Investing in Our Children's Future, Today / Francisco Nuñez Music as Therapy / Practitioners and performers relay first-hand experiences of therapeutic music interventions. Title TK / Zakir Hussain ; Healing Note by Note / Tom Sweitzer ; The Potential of Group Singing to Promote Health and Well-Being / Julene Johnson ; Sing Your Way Home ; Stacie Aamon Yedell ; ... & the Field : Re-membering the interlands in Music & Health / esperanza spalding and marisol norris ; Music and Memory / Connie Tomaino ; Her Voice : Music as a Form of Self-Expression in Cancer Treatment / Sheri Robb and Jefri Franks ; Achieving Body, Mind, and Soul: Harmony with Multiple Sclerosis / Courtney Platt
Science : A Deeper Dive-Scientists reveal their research methodology and compelling results. Arts-Based Therapies in Integrative Health / Emmeline Edwards, Wen Chen, Catherine Law, Mark Pitcher, and Helene M. Langevin ; Music, Memory, Aging and Science / Coryse St. Hillaire-Clarke, Lisa Onken, and David Frankowski ; Art Therapy, Psychology, and Neuroscience: A Timely Convergence / Anjan Chatterjee and Juliet King ; Coda and Crescendo: How Neuroscience Created Neurologic Music Therapy to Help Heal the Injured Brain / Michael Thaut ; Musical Enjoyment and the Reward Circuits of the Brain / Robert Zatorre ; The Benefits of Musical Engagement Across the Lifespan : Education, Health and Well Being / Antonio Damasio, Hanna Damasio, Assal Habibi, and Beatriz Ilari
The Road Ahead-Thought leaders outline an integrated approach to arts, technology, community, and health for the future. Common Nature : Humans as Cultural Beings / Yo-Yo Ma ; Composing the Future of Health / Tod Machover, Rébecca Kleinberger, and Alexandra Rieger ; Porous Density / Liz Diller ; Blueprint for Cultivating the Neuroarts Ecosystem / Susan Magsamen and Ruth Katz.Digital Access ProQuest Ebook Central 2024Limited to 1 simultaneous user