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  • Article
    Green DS, Jefferson M, Boots B, Stone L.
    J Hazard Mater. 2021 01 15;402:124070.
    Biodegradable plastics are becoming increasingly popular due to global concerns about plastic pollution. In this study, the impacts of glitter manufactured of conventional, non-biodegradable polyethylene terephthalate (PET) versus glitter of alternative materials (modified regenerated cellulose (MRC), mica or synthetic mica) on the biodiversity and ecosystem functioning of freshwater, lotic habitats were compared using a semi-natural mesocosm experiment. After 36 days, there was no effect of glitter on overall assemblage structure or diversity indices, however there was a two-fold increase in the abundance of New Zealand mud snails (Potamopyrgus antipodarum) in response to MRC glitter. In addition, the root length of common duckweed (Lemna minor) and phytoplankton biomass (based on chlorophyll content) were significantly reduced by exposure to any type of glitter. On the contrary, the chlorophyll content in the sediment (indicating microphytobenthos biomass) was significantly greater in those exposed to synthetic mica glitter. Organic matter content of sediment did not differ amongst any of the treatments. However initially, on days 8 and 15, NO32- concentration in the control treatment were significantly greater than in all glitter treatments, but this observation disappeared over time. Overall, results indicate that both conventional and alternative glitters can cause ecological impacts in aquatic ecosystems.
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  • Article
    Mohapatra S, Ranjan Mishra R, Nayak B, Chandra Behera B, Das Mohapatra PK.
    Bioresour Technol. 2020 Dec;318:124070.
    Escalating environmental concerns and petroleum demands leads into the present study. In this investigation delignification of rice straw was optimized by NaOH and H2SO4 pretreatment using L16 Taguchi orthogonal array. NaOH pretreatment revealed higher delignification as compared to H2SO4 and; further subjected to separate enzymatic hydrolysis and co-fermentation (SHCF) using RSM as the SHCF demonstrated a maximum glucose and xylose yield of 575 and 205 mg/g. Further, butanol concentration of 4.32 g/L was achieved from 20 g/L of sugar loadings by co-culture of Saccharomyces cerevisiae and Pichia sp. at 72 h of incubation time which was 79.25% higher as compared to monocultures of Pichia sp. Scale-up experiments with higher sugar loadings (90 g/L) demonstrated a butanol concentration of 13.3 g/L. The release of amino acids in co-culture and monoculture systems demonstrated that the addition of S. cerevisiae promoted the butanol synthesis pathway which led to higher butanol concentration.
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  • Article
    Junnila A, Mortier L, Arbiol A, Harju E, Tomberg T, Hirvonen J, Viitala T, Karttunen AP, Peltonen L.
    Int J Pharm. 2024 Apr 25;655:124070.
    The importance of ink rheology to the outcome of 3D printing is well recognized. However, rheological properties of printing inks containing drug nanocrystals have not been widely investigated. Therefore, the objective of this study was to establish a correlation between the composition of nanocrystal printing ink, the ink rheology, and the entire printing process. Indomethacin was used as a model poorly soluble drug to produce nanosuspensions with improved solubility properties through particle size reduction. The nanosuspensions were further developed into semisolid extrusion 3D printing inks with varying nanocrystal and poloxamer 407 concentrations. Nanocrystals were found to affect the rheological properties of the printing inks both by being less self-supporting and having higher yielding resistances. During printing, nozzle blockages occurred. Nevertheless, all inks were found to be printable. Finally, the rheological properties of the inks were successfully correlated with various printing and product properties. Overall, these experiments shed new light on the rheological properties of printing inks containing nanocrystals.
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  • Article
    Dhar G, Sen S, Chaudhuri G.
    PLoS One. 2015;10(4):e0124070.
    Aggressive cancers exhibit an efficient conversion of high amounts of glucose to lactate accompanied by acid secretion, a phenomenon popularly known as the Warburg effect. The acidic microenvironment and the alkaline cytosol create a proton-gradient (acid gradient) across the plasma membrane that represents proton-motive energy. Increasing experimental data from physiological relevant models suggest that acid gradient stimulates tumor proliferation, and can also support its energy needs. However, direct biochemical evidence linking extracellular acid gradient to generation of intracellular ATP are missing. In this work, we demonstrate that cancer cells can synthesize significant amounts of phosphate-bonds from phosphate in response to acid gradient across plasma membrane. The noted phenomenon exists in absence of glycolysis and mitochondrial ATP synthesis, and is unique to cancer. Biochemical assays using viable cancer cells, and purified plasma membrane vesicles utilizing radioactive phosphate, confirmed phosphate-bond synthesis from free phosphate (Pi), and also localization of this activity to the plasma membrane. In addition to ATP, predominant formation of pyrophosphate (PPi) from Pi was also observed when plasma membrane vesicles from cancer cells were subjected to trans-membrane acid gradient. Cancer cytosols were found capable of converting PPi to ATP, and also stimulate ATP synthesis from Pi from the vesicles. Acid gradient created through glucose metabolism by cancer cells, as observed in tumors, also proved critical for phosphate-bond synthesis. In brief, these observations reveal a role of acidic tumor milieu as a potential energy source and may offer a novel therapeutic target.
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  • Article
    Xu P, Yuan L, Wang K, Pan B, Ye Y, Lu K.
    Int J Biol Macromol. 2023 May 15;237:124070.
    Two peptide-carbazole conjugates, CTAT and CNLS, were designed and synthesized using carbazole Schiff base to modify the cell membrane penetrating peptide TAT (47-57) and the nuclear localization peptide NLS at the N terminus. The interaction with ctDNA was investigated by multispectral and agarose gel electrophoresis. And the effects of CNLS and CTAT on the G-quadruplex structure were explored by circular dichroism titration experiments. The results show that both CTAT and CNLS interact with ctDNA in a minor groove binding manner. Both conjugates bind more tightly to DNA than the individual substances CIBA, TAT and NLS. In addition, CTAT and CNLS are capable of unfolding parallel G-quadruplex structures and are potential G-quadruplex unfolding agents. Finally, broth microdilution was performed to test the antimicrobial activity of the peptides. The results showed that CTAT and CNLS had a 4-fold increase in antimicrobial activity compared with the parent peptides TAT and NLS. They could exert antimicrobial activity by disrupting the integrity of cell membrane bilayer and binding to DNA, and could be used as novel antimicrobial peptides for the development of novel antimicrobial antibiotics.
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  • Article
    Sharmeen S, Woolfork A, Hage DS.
    J Chromatogr B Analyt Technol Biomed Life Sci. 2024 Apr 01;1236:124070.
    Human serum albumin (HSA) is known to undergo modifications by glucose during diabetes. This process produces glycated HSA that can have altered binding to some drugs. In this study, high-performance affinity microcolumns and competition studies were used to see how glycation affects the binding by two thiazolidinedione-class drugs (i.e., pioglitazone and rosiglitazone) at specific regions of HSA. These regions included Sudlow sites I and II, the tamoxifen and digitoxin sites, and a drug-binding site located in subdomain IB. At Sudlow site II, the association equilibrium constants (or binding constants) for pioglitazone and rosiglitazone with normal HSA were 1.7 × 105 M-1 and 2.0 × 105 M-1 at pH 7.4 and 37 °C, with values that changed by up to 5.7-fold for glycated HSA. Sudlow site I of normal HSA had binding constants for pioglitazone and rosiglitazone of 3.4 × 105 M-1 and 4.6 × 105 M-1, with these values changing by up to 1.5-fold for glycated HSA. Rosiglitazone was found to also bind a second region that had a positive allosteric effect on Sudlow site I for all the tested preparations of HSA (binding affinity, 1.1-3.2 × 105 M-1; coupling constant for Sudlow site I, 1.20-1.34). Both drugs had a strong positive allosteric effect on the tamoxifen site of HSA (coupling constants, 13.7-19.9 for pioglitazone and 3.7-11.5 for rosiglitazone). Rosiglitazone also had weak interactions at a site in subdomain IB, with a binding constant of 1.4 × 103 M-1 for normal HSA and a value that was altered by up to 6.8-fold with glycated HSA. Neither of the tested drugs had any significant binding at the digitoxin site. The results were used to produce affinity maps that described binding by these thiazolidinediones with HSA and the effects of glycation on these interactions during diabetes.
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  • Book
    Christian U. Krägeloh, Marcus A. Henning, Oleg N. Medvedev, Xuan Joanna Feng, Fiona Moir, Rex Billington and Richard J. Siegert.
    Summary: This book provides an outline and critical discussion of the characteristics of mindfulness-based interventions (MBIs) research. Since the first reports on the use of mindfulness practices in health interventions, a large body of research literature has emerged to document the effectiveness of MBIs for reducing psychological distress and to increase well-being. The integration of mindfulness into very diverse psychological theories makes it a unique concept in psychology that has generated a large amount of interest both in academic research but also the broader media. With this growing literature, mindfulness researchers have also recognised the need to be more critical of its developments, such as how MBIs are presented to the public or what types of research methods are used to test claims of an MBI's effectiveness. This book examines the large variety of approaches in which MBIs have been studied, including an outline of the philosophical underpinnings of MBI research, definition and measurement of mindfulness, the use of qualitative and quantitative research methods, research design, and research that addresses cultural and religious factors. The book contributes to increased awareness of the current direction of MBI research and thus seeks to contribute to further methodological refinement and sophistication of the research field. This book on the characteristics of research on MBIs is a must read for any researcher or practitioner interested in this fascinating topic.

    Contents:
    The characteristics of research on mindfulness-based interventions
    Meta-theories and qualitative methods in mindfulness research
    Measuring mindfulness
    Quantitative research on the effectiveness of mindfulness-based interventions
    Cultural and religious factors in research on mindfulness-based interventions.
    Digital Access TandFonline 2019
doi:10.1016/j.jhazmat.2020.124070 doi:10.1016/j.biortech.2020.124070 doi:10.1016/j.ijpharm.2024.124070 doi:10.1371/journal.pone.0124070 doi:10.1016/j.ijbiomac.2023.124070 doi:10.1016/j.jchromb.2024.124070 doi:10.4324/9781315545875