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  • Article
    Zhuang Q, Xu J, Deng D, Chao T, Li J, Zhang R, Peng R, Liu Z.
    Biomaterials. 2021 01;268:120550.
    Nanoscale outer membrane vesicles (OMVs) secreted by Gram-negative bacteria are often applied in antibacterial treatment as adjuvants or antigens. Recently, OMVs have also been tested in a few anti-tumor treatment studies, in which OMVs are injected multiple times to achieve certain therapeutic effects, showing risks in repeated cytokine storms. Herein, we propose the use a single low dose of OMVs combined with photothermal therapy (PTT) for effective cancer treatment. It was found that single i. v. injection of OMVs could activate the immune system by boosting the secretion levels of anti-tumor related cytokines. In addition, single i. v. injection of OMVs could also lead to extravasation of red blood cells in the tumor mainly owing to the effect of lipopolysaccharide on the OMVs. Such effect was not observed in other normal organs. As the results, the tumors on OMV-treated mice showed obviously darkened color with greatly increased intratumoral optical absorbance in the near-infrared (NIR) region, further enabling effective photothermal ablation of those tumors by the NIR laser. Without causing obvious adverse responses, bacteria-derived OMVs may be a new type of therapeutic agent for cancer treatment with multiple functions.
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  • Article
    Wu T, Li X, Fu Y, Ding X, Li Z, Zhu G, Fan J.
    Talanta. 2020 Mar 01;209:120550.
    Developing the high selectivity and sensitivity strategy for nucleic acid detection is crucial for early diagnosis and therapy of diseases. In this work, a novel low back-ground fluorescent sensor platform for the detection of nucleic acid has been developed based on δ-FeOOH nanosheets integrating with exonuclease III-assisted target-recycling signal amplification. Because of the strong binding ability between the single-strand DNA (ssDNA) and the δ-FeOOH nanosheets, the dye-labeled ssDNA probe would be quenched by δ-FeOOH nanosheets through fluorescence resonance energy transfer (FRET). By using magnetic separate properties of δ-FeOOH, the background signal was separated from the sensor system, and the low background sensor system was obtained. After adding the target DNA, a double-strand DNA complex (dsDNA) would be formed between the target DNA and dye-labeled ssDNA probe. Then, the dye-labeled ssDNA probe in the dsDNA complex would be stepwise hydrolyzed into short fragments from 3'-terminus by Exonuclease III, and the fluorescence signal was recovered due to the weak bind affinity between the short fragments and δ-FeOOH nanosheets. By using the fluorescence quenching ability of δ-FeOOH nanosheets and enzyme-assisted target-recycling signal amplification, this strategy could show an excellent selectivity toward hepatitis C virus DNA with a low detection limit of 10 pM. By simply changing the dye-labeled ssDNA probe sequence, this sensing platform can be developed as a universal approach for the simple, sensitive, and selective detection of different target DNA.
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  • Article
    Di Dona G, Zamfira DA, Battista M, Battaglini L, Perani D, Ronconi L.
    Neuroimage. 2024 Apr 01;289:120550.
    Visual crowding is the difficulty in identifying an object when surrounded by neighbouring flankers, representing a bottleneck for object perception. Crowding arises not only from the activity of visual areas but also from parietal areas and fronto-parietal network activity. Parietal areas would provide the dorsal-to-ventral guidance for object identification and the fronto-parietal network would modulate the attentional resolution. Several studies highlighted the relevance of beta oscillations (15-25 Hz) in these areas for visual crowding and other connatural visual phenomena. In the present study, we investigated the differential contribution of beta oscillations in the parietal cortex and fronto-parietal network in the resolution of visual crowding. During a crowding task with letter stimuli, high-definition transcranial Alternating Current Stimulation (tACS) in the beta band (18 Hz) was delivered bilaterally on parietal sites, on the right fronto-parietal network, and in a sham regime. Resting-state EEG was recorded before and after stimulation to measure tACS-induced aftereffects. The influence of crowding was reduced only when tACS was delivered bilaterally on parietal sites. In this condition, beta power was reduced after the stimulation. Furthermore, the magnitude of tACS-induced aftereffects varied as a function of individual differences in beta oscillations. Results corroborate the link between parietal beta oscillations and visual crowding, providing fundamental insights on brain rhythms underlying the dorsal-to-ventral guidance in visual perception and suggesting that beta tACS can induce plastic changes in these areas. Remarkably, these findings open new possibilities for neuromodulatory interventions for disorders characterised by abnormal crowding, such as dyslexia.
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  • Article
    Piao X, Huang J, Sun Y, Zhao Y, Zheng B, Zhou Y, Yu H, Zhou R, Cullen PJ.
    Carbohydr Polym. 2023 Apr 01;305:120550.
    Inulin is a prebiotic carbohydrate widely used in food industry due to its health benefits and unique rheological properties. For the first time, this study explores the potential of natural inulin as a sustainable food additive to enhance surimi gel characteristics, specifically focusing on understanding its molecular weight effects. The good solubility of inulin facilitates the conversion of α-helix to other secondary conformations which are favorable for protein denaturation and aggregation during gelation. Moreover, the abundant -OH groups at the surface of inulin can boost the chemical forces within surimi proteins to reinforce the gel network. Compared to short-chain inulin, long-chain inulin can alleviate proteolysis, enhance hydrophobic interactions and intertwine with myosin molecules, thereby reinforcing the gel network. A more viscous long-chain inulin solution formed within surimi gels fills the space between aggregated proteins and facilitates the lock of water molecules, improving the water-holding capacity (WHC). Thus, an addition of 12 % long-chain inulin leads to an enhanced hardness of surimi gel from 943 to 1593 and improved WHC from 72 % to 85 %. A new inulin-myosin interaction mechanism model is also proposed to provide useful guidelines for surimi processing and expanding the application of inulin within the food industries.
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  • Article
    Li R, Yao J, Liu J, Sunahara G, Duran R, Xi B, El-Saadani Z.
    J Environ Manage. 2024 Apr;356:120550.
    Acid mine drainage (AMD) caused environmental risks from heavy metal pollution, requiring treatment methods such as chemical precipitation and biological treatment. Monitoring and adapting treatment processes was crucial for success, but cost-effective pollution monitoring methods were lacking. Using bioindicators measured through 16S rRNA was a promising method to assess environmental pollution. This study evaluated the effects of AMD on ecological health using the ecological risk index (RI) and the Risk Assessment Code (RAC) indices. Additionally, we also examined how acidic metal stress affected the diversity of bacteria and fungi, as well as their networks. Bioindicators were identified using linear discriminant analysis effect size (LEfSe), Partial least squares regression (PLS-R), and Spearman analyses. The study found that Cd, Cu, Pb, and As pose potential ecological risks in that order. Fungal diversity decreased by 44.88% in AMD-affected areas, more than the 33.61% decrease in bacterial diversity. Microbial diversity was positively correlated with pH (r = 0.88, p = 0.04) and negatively correlated with bioavailable metal concentrations (r = -0.59, p = 0.05). Similarly, microbial diversity was negatively correlated with bioavailable metal concentrations (bio_Cu, bio_Pb, bio_Cd) (r = 0.79, p = 0.03). Acidiferrobacter and Thermoplasmataceae were prevalent in acidic metal environments, while Puia and Chitinophagaceae were identified as biomarker species in the control area (LDA>4). Acidiferrobacter and Thermoplasmataceae were found to be pH-tolerant bioindicators with high reliability (r = 1, P < 0.05, BW > 0.1) through PLS-R and Spearman analysis. Conversely, Puia and Chitinophagaceae were pH-sensitive bioindicators, while Teratosphaeriaceae was a potential bioindicator for Cu-Zn-Cd metal pollution. This study identified bioindicator species for acid and metal pollution in AMD habitats. This study outlined the focus of biological monitoring in AMD acidic stress environments, including extreme pH, heavy metal pollutants, and indicator species. It also provided essential information for heavy metal bioremediation, such as the role of omics and the effects of organic matter on metal bioavailability.
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  • Article
    Powell A, Berry DS, Salinas M, Louis ED.
    J Neurol Sci. 2023 02 15;445:120550.
    BACKGROUND: Essential tremor (ET) is a highly prevalent movement disorder. As the diagnosis is purely clinical, it is important for clinicians to develop a reliable taxonomy of signs and symptoms of ET. Here, we investigate two common clinical maneuvers for determining the presence and severity of postural tremor in ET cases: the evaluation of tremor observed when arms are held in the outstretched and wing posture positions.
    METHODS: Two movement disorders neurologists assessed tremor severity from videotaped neurological evaluations of 100 ET cases displaying outstretched and wing posture positions. These raters also completed a forced choice measure indicating whether upper limb tremor was more severe in the outstretched position, the wing posture position, or equivalent in the two positions.
    RESULTS: Our raters judged tremor observed in the wing posture position to be more severe than tremor observed in the outstretched position (average ratings of 1.17 and 1.03, respectively, p's <0.05). This finding replicated across the two independent neurologists' ratings of tremor severity. Analyses of the forced choice measure revealed parallel results, with tremor observed in the wing posture position more often identified as more severe than tremor observed in the outstretched position (37.5% versus 14.5%, respectively, p < 0.05).
    CONCLUSION: Our data reveal that ET cases generally display more severe upper limb tremor in the wing posture position than in the outstretched position. These findings having potential clinical and research implications, suggesting that postural tremor in ET may be more easily identified from observations of the wing posture than the outstretched position.
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  • Article
    Veludo AF, Stroobandt B, Van Bladel H, Sandoval-Diez N, Guxens M, Joseph W, Röösli M.
    Environ Res. 2025 Feb 01;266:120550.
    The advancement of cellular networks requires updating measurement protocols to better study radiofrequency electromagnetic field (RF-EMF) exposure emitted from devices and base stations. This paper aims to present a novel activity-based microenvironmental survey protocol to measure environmental, auto-induced downlink (DL), and uplink (UL) RF-EMF exposure in the era of 5G. We present results when applying the protocol in Switzerland. Five study areas with different degrees of urbanization were selected, in which microenvironments were defined to assess RF-EMF exposure in the population. Three scenarios of data transmission were performed using a user equipment in flight mode (non-user), inducing DL traffic (max DL), or UL traffic (max UL). The exposimeter ExpoM-RF 4, continuously measuring 35 frequency bands ranging from broadcasting to Wi-Fi sources, was carried in a backpack and placed 30 cm apart from the user equipment. The highest median RF-EMF levels during the non-user scenario were measured in an urban business area (1.02 mW/m2). Here, DL and broadcasting bands contributed the most to total RF-EMF levels. Compared to the non-user scenario, exposure levels increased substantially during max DL due to the 5G band at 3.5 GHz with 50% of the median levels between 3.20 and 12.13 mW/m2, mostly in urban areas. Note that the time-division nature of this band prevents distinguishing between exposure contribution from DL beamforming or UL signals emitted at this frequency. The highest levels were measured during max UL, especially in rural microenvironments, with 50% of the median levels between 12.08 and 37.50 mW/m2. Mobile UL 2.1 GHz band was the primary contributor to exposure during this scenario. The protocol was successfully applied in Switzerland and used in nine additional countries. Inducing DL and UL traffic resulted in a substantial increase in exposure, whereas environmental exposure levels remained similar to previous studies. This data is important for epidemiological research and risk communication/management.
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  • Article
    Li L, Li X, The E, Wang LJ, Yuan TY, Wang SY, Feng J, Wang J, Liu Y, Wu YH, Ma XE, Ge J, Cui YY, Jiang XY.
    PLoS One. 2015;10(3):e0120550.
    The cellular mechanisms of primary varicose great saphenous veins (GSVs) involve inflammation, apoptosis, and proliferation of local cells and extracellular matrix degradation. Long non-coding RNAs (lncRNAs) play important roles in these cellular processes; however, which and how lncRNAs related to these mechanisms take effect on GSVs remain unclear. By screening lncRNAs that might experience changes in GSV varicosities, we selected the lower expressed lncRNA-GAS5 (growth arrest specific transcript 5) for functional assessments. Silencing of lncRNA-GAS5 promoted cell proliferation and migration, and cell cycle of the human saphenous vein smooth muscle cells (HSVSMCs), whereas overexpressing it inhibited these cellular behaviors and reduced apoptosis of HSVSMCs. RNA pull-down experiment revealed a direct bind of lncRNA-GAS5 to a Ca2+-dependent RNA-binding protein, Annexin A2. Further experiments showed that silencing of Annexin A2 reduced the HSVSMCs proliferation and vice versa. In the context of lncRNA-GAS5 knockdown, silencing of Annexin A2 reduced the proliferation of HSVSMCs while overexpression of Annexin A2 increased the proliferation. Thus, the low expression of lncRNA-GAS5 may facilitate HSVSMCs proliferation and migration through Annexin A2 and thereby the pathogenesis of GSV varicosities.
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  • Article
    Huang M, Xie L, Wang Y, Feng X, Gao J, Lou Z, Xiong Y.
    Environ Pollut. 2023 Jan 01;316(Pt 2):120550.
    Uranium extraction from radioactive nuclear waste is vital for sustainable energy supply and ecological security. Herein, a polyethyleneimine-chitosan composite microspheres n-PEI/ECH-CTS (n = 0.1, 0.2, 0.3, 0.4, 0.5) were synthetized for efficient and selective uranium adsorption. The prepared chitosan microspheres with uniform size, uniform dispersion and good mechanical strength combine cost-effectiveness and environmental benefits. The 0.4-PEI/ECH-CTS exhibits the highest adsorption capacity of 380.65 mg g-1 within only 4 h due to high nitrogen content of 6.57 mol kg-1. The DFT calculations confirms that the optimal coordination mode of UO22+ and 0.4-PEI/ECH-CTS is one UO22+ chelated with two -NH2 from two adsorption units, respectively. Adsorption efficiency of U(VI) from simulated nuclear wastewater achieves to 100%, and the Kd value is up to 1.1 × 104 mL g-1, which is 1.7 × 104-6.1 × 104 times that of coexisting ions. The CU(VI) reduces in simulated wastewater from 10.98 mg L-1 to 1 μg L-1, which is well below the US Environmental Protection Agency uranium limits for drinking water (30 μg L-1). Besides, 0.4-PEI/ECH-CTS still maintains above 95% adsorption efficiency after seven cycles. In short, the 0.4-PEI/ECH-CTS microspheres integrate high performance, practicality and cost-effectiveness, which has great advantages in practical industrial applications.
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  • Article
    Qiu L, Ouyang Y, Feng Y, Zhang X, Wang X.
    Int J Heat Mass Transf. 2020 Dec;163:120550.
    Thermophysical properties of human skin surface and subsurface can reflect the hydration state of the skin and the blood flow rate in the near surface microvessels, which reveals important physiological information related to dermatology and overall health status of human body. Although a few techniques have been developed to measure these signs, complicated devices are required and the subjects need to be completely fixed during the test period. Here, a flexible thermosensor-based 3ω technology was used to monitor thermal conductivity of human skins at different states. Through the analysis of these characteristics, the corresponding physiological state can be established, which can provide a new detection method for the evaluation or prediction of human health status.
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  • Article
    Henry B, Samokhvalov A.
    Spectrochim Acta A Mol Biomol Spectrosc. 2022 Feb 15;267(Pt 2):120550.
    Metal-organic frameworks (MOFs) are advanced highly porous coordination polymers of high interest to separations, environmental remediation, catalysis, and biomedicine. While many MOFs are unstable in water and aqueous solutions, aluminum MOFs (Al-MOFs) offer an unprecedented stability. First, we synthesize unusual highly hygroscopic Al-MOF MIL-160(Al), purify it and assign FTIR peaks to specific groups as potential water binding sites. Further, we introduce a novel method of in-situ time-dependent ATR-FTIR spectroscopy to detect specific binding sites in MIL-160(Al) and investigate the progress of reaction. Specifically, we combine in-situ time-dependent ATR-FTIR spectroscopy with using water as "spectroscopic probe" to determine binding sites in MIL-160(Al) and their evolution during the reaction. The in-situ time-dependent ATR-FTIR spectra provide evidence of water bonding to: the μ-OH group, the carboxylate anion COO- in 2,5-FDCA2- linker, oxygen atom in the furan ring of the linker, and the C-C and C-H bonds of the furan ring of the linker. Then, we conduct mechanistic and kinetic study of sorption of water vapor on MIL-160(Al) in air using the combination of two complementary in-situ time-dependent methods: the ATR-FTIR spectroscopy and gravimetric analysis. Water vapor sorption on MIL-160(Al) results in the solid-state adsorption complex with up to four water molecules per unit of MIL-160(Al). Chemical kinetics of water sorption on MIL-160(Al) follows a pseudo-first order rate law and it is consistent with dynamics and timescale revealed by in-situ time-dependent ATR-FTIR. The combination of two in-situ time-dependent methods, the ATR-FTIR spectroscopy and gravimetry, forms a new powerful experimental approach to facilely study mechanisms, stoichiometry and chemical kinetics of various solid-gas reactions in the ambient and controlled environments.
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  • Book
    edited by Rishabha Malviya, Pramod Kumar Sharma, Sonali Sundram, Rajesh Kumar Dhanaraj, Balamurugan Balusamy.
    Summary: "Nowadays, Raw biological data can be easily stored as databases in the computers but extracting the required information from the quantum of data is the actual challenge for researchers. For this reason, bioinformatics tools perform a vital role in extracting and analyzing information from the databases. Bioinformatic Tools and Big Data Analytics for Patient Care: Future of Healthcare describe the applications of bioinformatics, data management and computational techniques in clinical studies and drug discovery for patient care. This book gives details about the recent developments in the fields of artificial intelligence, cloud computing and data analytics for improved patient care. This book highlights the advancement in computational techniques which are used to perform intelligent medical tasks"-- Provided by publisher.

    Contents:
    Role of bioinformatics tools and technologies in clinical trial
    Bioinformatics Tools and Software in Clinical Research
    Computational Biology for Clinical Research
    Issues and challenges related to CBTs for clinical research
    Artificial Intelligence : An Emerging Technique in Pharma & Health care system
    AI in Healthcare and its application in Brain Stroke Diagnosis
    Computational Cloud Infrastructure for Patient Care
    Advancement in Gene Delivery : The Role of Bioinformatics
    Drug Development using Cloud Application
    Cloud Application in Drug Development
    Framework for handling medical data in research.
    Digital Access TandFonline 2022
doi:10.1016/j.biomaterials.2020.120550 doi:10.1016/j.talanta.2019.120550 doi:10.1016/j.neuroimage.2024.120550 doi:10.1016/j.carbpol.2023.120550 doi:10.1016/j.jenvman.2024.120550 doi:10.1016/j.jns.2023.120550 doi:10.1016/j.envres.2024.120550 doi:10.1371/journal.pone.0120550 doi:10.1016/j.envpol.2022.120550 doi:10.1016/j.ijheatmasstransfer.2020.120550 doi:10.1016/j.saa.2021.120550