Today's Hours: 10:00am - 6:00pm

Search

Did You Mean:

Search Results

  • Article
    Zhang W, Chen Y, Hou Y, Wang W, Liu H, Zheng L.
    Ultramicroscopy. 2019 Dec;207:112832.
    Several subsurface imaging methods based on atomic force microscopy (AFM) linear nanomechanical mapping, namely contact resonance (CR), bimodal and harmonic AFMs, are investigated and compared. Their respective subsurface detection capability is estimated and evaluated on a model specimen, which is prepared by embedding SiO2 microparticles in a PDMS elastomer. The measured CR frequency, bimodal and harmonic amplitudes are related to local mechanical properties by analyzing cantilever dynamics and further linked to subsurface depths of the particles by finite element analysis. The maximum detectable depths are obtained from the apparent particle diameters in subsurface image channels via employing a simple geometrical model. Under common experimental settings, results demonstrate that the depth limits reach up to about 812 nm, 212 nm and 127 nm for CR, bimodal and harmonic AFM modes, respectively. The depth sensitivity can be tuned and optimized by using either different cantilever eigenmodes in CR-AFM or spectroscopy analysis in bimodal and harmonic AFMs. The three imaging methods have their own suitable application situations. The comparisons can advance a further step into understanding the subsurface image contrast via AFM mechanical sensing.
    Digital Access Direct to PDF
  • Article
    Wu C, Deng W, Shan W, Liu X, Zhu L, Cai D, Wei W, Yang Y, Chen J, Lu W, Kuang J.
    Cell Rep. 2023 08 29;42(8):112832.
    The mitogen-activated protein kinase (MAPK) cascade consisting of MKKK, MKK, and MPK plays an indispensable role in various plant physiological processes. Previously, we showed that phosphorylation of MabZIP21 by MaMPK6-3 is involved in banana fruit ripening, but the regulatory mechanism by which MKK controls banana fruit ripening remains unclear. Here, ripening-induced MaMKK1 from banana fruit is characterized, and transiently overexpressing and silencing of MaMKK1 in banana fruit accelerates and inhibits fruit ripening, respectively, possibly by influencing phosphorylation and activity of MPK. MaMKK1 interacts with and phosphorylates MaMPK6-3 and MaMPK11-4 mainly at the pTEpY residues, resulting in MPK activation. MaMPK11-4 phosphorylates MabZIP21 to elevate its transcriptional activation ability. Transgenic tomato fruit expressing MabZIP21 ripen quickly with a concomitant increase in MabZIP21 phosphorylation. Additionally, MabZIP21 activates MaMPK11-4 and MaMKK1 transcription to form a regulatory feedback loop. Collectively, here we report a regulatory pathway of the MaMPK6-3/11-4-MabZIP21 module in controlling banana fruit ripening.
    Digital Access Access Options
  • Article
    Ji Y, Liu B, Song J, Cheng J, Wang H, Su H.
    Environ Res. 2022 06;209:112832.
    Motor vehicle exhaust emissions have become the main source of urban air pollution in China, but few studies have explored the association of short-term exposure to traffic-related air pollutants (TRAPs) with anxiety disorders. Thus, we used an overdispersed, generalized additive model (GAM) to investigate the association between TRAPs and hospital admissions (HAs) for anxiety in Qingdao, a coastal Chinese city with high vehicle ownership. In addition, stratified analyses were performed by gender, age, season and hospitalization frequency (first admission and readmission). A positive association between TRAPs and HAs for anxiety was observed. Both inhalable particulate matter (PM10) and nitrogen dioxide (NO2) showed significant effects at lag 3 in the single-day lag structure, and each 10 μg/m3 increase in the concentrations was significantly associated with increases of 0.88% [95% confidence interval (CI): 0.04%, 1.72%] for PM10 and 2.74% (0.45%, 5.08%) for NO2 on anxiety hospitalizations. For fine particulate matter (PM2.5) and carbon monoxide (CO), the strongest effects were found at lag05 and lag04 [2.67% (0.77%, 4.62%) and 0.19% (0.04%, 0.34%), respectively] in the multiday lag structure. The estimates of PM2.5 were relatively robust after adjusting for other pollutants in the two-pollutant model. Stratified analyses indicated that the associations were stronger in females and younger individuals (<45 in age) than in males and elderly individuals (≥45 in age). Furthermore, the effects of PM2.5 and CO were most obvious during the cold season. Regarding hospitalization frequency, only PM2.5 was found to have a significant effect in the first-admission group. The results showed that short-term exposure to TRAPs, especially to PM2.5, was significantly associated with the increased risk of daily HAs for anxiety, which can help clinicians and policymakers better understand the effects of TRAPs to implement targeted interventions.
    Digital Access Access Options
  • Article
    Tan Y, Tang F, Li J, Yu H, Wu M, Wu Y, Zeng H, Hou K, Zhang Q.
    Biomed Pharmacother. 2022 May;149:112832.
    Cutaneous melanoma is an aggressive cancer type derived from melanocytes and its incidence has rapidly increased worldwide. Despite the vast improvement in therapy, melanoma is still confronted with high invasion, metastasis, and recurrence rate. Recent studies have confirmed that the exosomes are naturally occurring membranous extracellular vesicles with nano-sized lipid bilayers, performing as information messagers within cellular reciprocal action. Exosomes are unquestionably endowed with multifaceted roles in various diseases, including melanoma. Notably, tumor-derived exosomes play a pivotal role in conditioning the tumor microenvironment to promote the growth, metastasis, immune escape, and even drug-resistance of melanoma by transferring carcinogenic nucleic acids and proteins. Clinically, the dynamic expressions of exosomal components and loadings in melanoma patients with different tumor stages confer the clinical application of melanoma exosomes as diagnostic biomarkers. Hence, this review highlights the recent complicated roles and mechanisms of melanoma exosomes, as well as their potential as diagnostic and therapeutic targets in melanoma. The in-depth insights into the properties and behaviors of melanoma exosomes are of great potential to yield attractive therapeutic methods for melanoma.
    Digital Access Access Options
  • Article
    Li F, Bai Y, Guan Z, Ji X, Zhan X, Gao Y, Zhong W, Rao Z.
    Int Immunopharmacol. 2024 Dec 05;142(Pt A):112832.
    BACKGROUND: The lungs are highly susceptible to damage during sepsis, with severe lung injury potentially progressing to acute respiratory distress syndrome and even fatal sepsis. Effective efferocytosis of apoptotic cells is crucial in alleviating inflammation and tissue injury.
    METHODS: We established a septic lung injury mouse model via intraperitoneal injection of lipopolysaccharide. Lung injury was assessed by histology, immunofluorescence, neutrophil immunohistochemistry staining, and cytokine detection. We extracted alveolar macrophages by bronchoalveolar lavage and primary macrophages from mouse bone marrow to investigate the regulatory effects of Dexmedetomidine (DEX) on efferocytosis. We further validated the molecular mechanisms underlying the regulation of macrophage efferocytosis by DEX through knockdown of AXL expression. Additionally, we examined the efferocytic ability of monocytes isolated from patients.
    RESULTS: We discovered that DEX treatment effectively alleviated pulmonary injury and inflammation. Lipopolysaccharide reduced macrophage efferocytosis and AXL expression which were reversed by DEX. We also found DEX inhibited the increased activation of A Disintegrin And Metalloproteinase 10 (ADAM10) and the production of soluble AXL. Moreover, our findings demonstrated that DEX decreased the elevated ROS production linked to higher ADAM10 activation. Blocking AXL negated DEX's benefits on efferocytosis and lung protection. Efferocytosis in monocytes from septic lung injury patients was notably lower than in healthy individuals.
    CONCLUSION: Our findings demonstrated that DEX treatment effectively reduces septic lung injury by promoting macrophage efferocytosis through ROS/ADAM10/AXL signaling pathwway.
    Digital Access Access Options
  • Article
    Howard S, Fitzgerald G, Gallagher S.
    Physiol Behav. 2020 05 15;219:112832.
    While hospital admissions for cardiac causes on a population level appear to rise when the national team is playing in a sporting fixture, little research has examined cardiovascular reactions in spectators watching a sporting event. In a sample of 41 male supporters of a rugby team, all of whom had played the sport themselves, cardiovascular levels and psychological ratings of stress and anxiety were measured pre- and post-match. Counterfactual thinking was also assessed. Mixed factorial ANOVA analyses showed that in supporters who had recently retired from playing, psychological ratings of stress and anxiety were significantly lower pre-match compared to those who had long-retired (all ps < 0.001). However, significant phase × player status interactions identified that systolic blood pressure, F(1, 39) = 13.93, p = .001, partial ƞ2 = 0.26, diastolic blood pressure, F(1,39) = 24.62, p < .001, partial η2 = 39, and heart rate, F(1, 39) = 23.02, p < .001, partial η2 = 0.37, increased significantly in recent-retired players compared to long-retired players, indicating that they found watching the match more stressful than the long-retired players. Furthermore, this effect was moderated by counterfactual thinking in recent-retired players only, where greater levels of counterfactual thinking was associated with greater cardiovascular reactivity. These findings confirm that watching a sporting fixture induces cardiovascular reactivity in spectators who recently retired from the sport, with counterfactual thinking a possible underlying pathway.
    Digital Access Access Options
  • Article
    Li Y, Dalabasmaz S, Gensberger-Reigl S, Heymich ML, Krofta K, Pischetsrieder M.
    Food Res Int. 2023 07;169:112832.
    Hop is widely used in beer brewing and as a medicinal product. The present study comprehensively analyzed the main molecular determinants of the antibacterial activity of hop extracts. Minimum inhibitory concentrations (MIC) against Bacillus subtilis between 31.25 and 250 µg/mL were found in the ethanolic extracts of five hop varieties for beer brewing, but not in the tea hop sample. Activity-guided fractionation revealed the highest antibacterial activity for lupulone and adlupulone (MIC 0.98 µg/mL). Metabolome profiling and subsequent multistep statistical analysis detected 33 metabolites out of 1826 features to be associated with the antibacterial activity including humulone, adhumulone, colupulone, lupulone, and adlupulone. Xanthohumol, the three humulone- and three lupulone congeners were quantified in the hop extracts by a validated ultrahigh-performance liquid chromatography-mass spectrometry method. Considering concentrations and MICs, colupulone and lupulone were identified as major contributors to the antibacterial activity of hop extract with the highest antibacterial activity values (concentration/MIC) of 1.59 and 2.56.
    Digital Access Access Options
  • Article
    Zhu YL, Li SL, Zhu CY, Wang W, Zuo WF, Qiu XJ.
    J Ethnopharmacol. 2020 Oct 05;260:112832.
    ETHNOPHARMACOLOGICAL RELEVANCE: Danzhi Xiaoyao Powder (DZXY) is a classical prescription, that has been extensively used in traditional Chinese medicine (TMC) to treat depression for many years. However, the mechanism of DZXY is still unclear.
    AIM OF THE STUDY: The aim was to investigate the mechanism of the antidepressant effect of DZXY on a rat model of chronic unpredictable mild stress (CUMS).
    MATERIALS AND METHODS: Forty male SD (Sprague-Dawley) rats with similar open field test (OFT) results were randomLy divided into a control group (n = 10) and an experimental group (n = 30). A depression model was established in the experimental group using the CUMS method. After the CUMS model was established successfully, the rats were randomLy divided into a depression model group and a DZXY group. The DZXY group was fed DZXY, while the depression model group and control group were given an equal amount of 0.5% sodium carboxymethyl cellulose suspension. Intragastric administration was performed once daily for 14 consecutive days. Animal weight, the sugar preference test, the open field test and the forced swimming test were used to evaluate the modeling effect and the antidepressant effect of DZXY. After the experiment, the plasma of rats was collected and the changes in plasma metabolites were analyzed by UPLC/Q-TOF-MS. The UPLC/Q-TOF-MS spectra data were evaluated by pattern recognition analysis to determine the changes in endogenous metabolites in the rat plasma samples.
    RESULTS: The results of the behavioral investigation showed that the rat model of depression was successfully replicated and that DZXY had an antidepressant effect. Using the UPLC-MS/MS metabolomics platform, partial least squares (PLS) and orthogonal partial least squares (OPLS), metabolic profile models (R2 and Q2 ≥ 0.5) of rat plasma were successfully constructed. The model could distinguish among the control group, the depression model group and the DZXY group. Finally, 38 differential metabolites were identified in the plasma. According to KEGG (http://www.kegg.jp) pathway analysis, amino acid metabolism, lipid metabolism, purine metabolism, the prolactin signaling pathway and bile secretion were enriched and represented the main metabolic pathways influenced in the plasma.
    CONCLUSIONS: This study successfully established a CUMS depression model. A total of 38 differential metabolites associated with depression were identified in the plasma of rats, 24 of which were modulated by DZXY. These results suggest that DZXY can improve excitability and play an antidepressant role by regulating phenylalanine metabolism, arachidonic acid metabolism, porphyrin metabolism, D-arginine and D-ornithine metabolism, steroid hormone biosynthesis, unsaturated fatty acid biosynthesis and steroid biosynthesis.
    Digital Access Access Options
  • Article
    Kumar PK, Mitra P, Ghosh R, Sharma S, Nebhinani N, Sharma P.
    Behav Brain Res. 2020 09 15;394:112832.
    Schizophrenia is a severe neuropsychiatric disorder affecting 1% of the world population. Disturbances in neuronal development and synaptic connections are important factors in the pathogenesis of schizophrenia. Brain derived neurotrophic factor (BDNF), a member of the neurotrophin family, plays a critical role in the development of neurons. Among several polymorphisms reported in BDNF, the rs6265 polymorphism is known to be associated with many neuropsychiatric diseases. This study was aimed to determine the effect of BDNF rs6265 functional polymorphism on serum BDNF concentration in patients with schizophrenia. In total, 50 schizophrenia patients and 50 controls were recruited after obtaining written informed consent. Serum BDNF levels were estimated using the ELISA method and BDNF rs6265 polymorphism was genotyped using T-ARMS PCR. Serum BDNF levels were decreased significantly in schizophrenia patients when compared to the healthy controls (p < 0.0001). Further, the rs6265 polymorphism was also not associated with the schizophrenia (p = 0.41). Intragroup analysis between different genotypes revealed no association between the serum BDNF levels and rs6265 polymorphism. Our results suggest that the functional polymorphism rs6265 is not associated with serum BDNF levels, which is in line with previous findings, which indicates that serum BDNF levels depend more on diagnostic effect than genetic effect. Replication studies on a larger study population are needed.
    Digital Access Access Options
  • Article
    Moreira MM, Neto MLR.
    Psychiatry Res. 2020 Jan 29;285:112832.
    Digital Access Access Options
  • Article
    Khorshidi S, Younesi S, Karkhaneh A.
    Colloids Surf B Biointerfaces. 2022 Nov;219:112832.
    Hypoxia is a serious obstacle in cancer treatment. The aberrant vascular network as well as the abnormal extracellular matrix arrangement results in formation of a hypoxic regions in tumors which show high resistance to the curing. Hypoxia makes the cancer treatment challengeable via two mechanisms; first and foremost, hypoxia changes the cell metabolism and leads the cells towards an aggressive and metastatic phenotype and second, hypoxia decreases the efficiency of the various cancer treatment modalities. Most of the cancer treatment methods including chemotherapy, radiotherapy, photodynamic therapy, sonodynamic therapy and immunotherapy are negatively affected by the oxygen deprivation. Therefore, the regional oxygenation is requisite to alleviate the negative impacts of the hypoxia on tumor cells and tumor therapy modalities. A great deal of effort has been put forth to resolve the problem of hypoxia in tumors. Peroxides have gained tremendous attention as oxygen generating components in cancer therapy. The concurrent loading of the peroxides and cancer treatment components into a single delivery system can bring about a multipurpose delivery system and substantially encourage the success of the cancer amelioration. In this review, we have tried to after the description of a relation between hypoxia and cancer treatment modalities, discuss the role of peroxides in tumor hyperoxygenation and cancer therapy success. Thereafter, we have summarized a number of vehicles for the delivery of the peroxide alone or in combination with other therapeutic components for cancer treatment.
    Digital Access Access Options
  • Article
    Liu X, Zhao Y, Li F.
    Biosens Bioelectron. 2021 Feb 01;173:112832.
    This work established an immobilization-free photoelectrochemical (PEC) biosensor for ultrasensitive determination of carcinoembryonic antigen (CEA) based on the DNA-functionalized metal-organic frameworks (MOFs) and T7 exonuclease-aided recycling amplification. In this proposal, MOFs were served as nanocarriers for efficient encapsulation of electron donors, while an ingeniously designed hairpin probe (HP) employed as the recognition element. The recognition of CEA by its aptamer sequence in HP triggered the conformational change and the T7 exonuclease-aided recycling amplification, which opened the pore of MOFs to release a large number of electron donors, producing a significantly increased photocurrent. Benefitting from the high loading ability of MOFs and the excellent amplification efficiency of the T7 exonuclease-assisted recycling process, the proposed biosensor is capable of ultrasensitive and highly selective determination of CEA with a detection limit down to 0.36 fg mL-1 and a wide linear range from 1.0 fg mL-1 to 10 ng mL-1. Moreover, the proposed biosensor can also apply to measure CEA in spiked serum samples, indicating that this PEC biosensor holds excellent potential for application in bioanalysis and early disease diagnosis.
    Digital Access Access Options
  • Article
    Cao S, Xie Y, Lu X, Zhao Z, Zhou F, Wang J, Liang L.
    J Inorg Biochem. 2025 Apr;265:112832.
    Two Gd(III) complexes [GdL(H2O)(NO3)2(CH3OH)0.75(CH3CH2OH)0.25] (Gd1) and [Gd2(OOCCH3)2L2(H2O)6]•2(H2O) (Gd2) (HL = 2-pyridylcarboxaldehyde isonicotinoylhydrazone) were synthesized with a Schiff base ligand. Crystallographic study reveals both Gd1 and Gd2 have a zero-dimensional mononuclear or binuclear structure. Magnetic investigations demonstrate that Gd1 and Gd2 exhibit potential magnetocaloric effects due to Gd(III) ions, which provide negligible magnetic anisotropy, and possess low-lying excited spin states. The antiproliferative activity of Gd1 and Gd2 to three tumor cell lines was conducted and the results showed Gd1 and Gd2 showed more pronounced antiproliferative activity to A549 cells better than cisplatin. The administration of Gd1 and Gd2 led to an increase in apoptosis among A549 cells in a concentration-dependent manner, along with a corresponding rise in the levels of reactive oxygen species (ROS) within the cells. Besides, Gd1 and Gd2 were able to significantly inhibit tumor cell migration. Cell cycle assay in A549 cells revealed that cell cycle was arrested of G0/G1 phase. Western blotting analysis showed that Gd1 and Gd2 complexes could promote apoptosis in A549 cells by modulating the expression of Bcl-2 and Bax proteins.
    Digital Access Access Options
  • Article
    Aranke M, Pham CT, Yilmaz M, Wang JK, Orhurhu V, An D, Cornett EM, Kaye AD, Ngo AL, Imani F, Farahmand Rad R, Varrassi G, Viswanath O, Urits I.
    Anesth Pain Med. 2021 Feb;11(1):e112832.
    In the US, an estimated 1 - 2% of chronic venous insufficiency (CVI) patients (of 6 - 7 million nationwide) develop at least one venous stasis ulcer (VSU) during their illness. Of these, approximately 40% develop subsequent ulcers, making VSU prognostically poor. Current management of VSU is costly, with poor prognosis, high recurrence rate, inadequate pain management, and significantly reduced quality of life (QoL). Topical volatile anesthetic agents, such as sevoflurane, offer improved pain relief and symptom control in patients suffering from chronic VSU. The immediate impact of topical sevoflurane in reducing pain associated with ulcer bed debridement has several implications in improving the quality of life in patients with CVI induced ulcers and in the prognosis and healing of the ulcers. This review summarizes a topical formulation of a volatile anesthetic and its implications for the management of VSUs.
    Digital Access Access Options
  • Article
    Fu J, Tang J, Han J, Hong Z.
    PLoS One. 2014;9(11):e112832.
    White matter lesions (WMLs) in normal elderly are related to chronic ischemia, and progression of WML occurs mostly in moderate to severe disease. However, the mechanism is uncertain. Thus, we enrolled fifty-six normal elderly patients without large artery disease. The severity of WML on MRI was graded as grade 0, I, II and III using the modified Fazekas scale. Cerebral blood flow (CBF) was measured by Xenon-CT. We found that CBF (mL/100 g/min) within periventricular lesions and in the right and left centrum semiovales were 20.33, 21.27 and 21.03, respectively, in group I; 16.33, 15.55 and 15.91, respectively, in group II; and 14.05, 14.46 and 14.23, respectively, in group III. CBF of normal-appearing white matter (NAWM) around periventricular areas and in the right and left centrum semiovales were 20.79, 22.26 and 22.15, respectively, in group 0; 21.12, 22.17 and 22.25, respectively, in group I; 18.02, 19.45 and 19.62, respectively, in group II; and 16.38, 18.18 and 16.74, respectively, in group III. Significant reductions in CBF were observed not only within lesions but also in NAWM surrounding the lesions. In addition, CBF was reduced significantly within lesions compared to NAWM of the same grade. Furthermore, CBF was reduced significantly in NAWM in grades II and III when compared to grades 0 and I. Our finding indicates that ischemia may play a role in the pathogenesis of WML. Additionally, our finding provides an alternative explanation for finding that the progression of WML occurred more commonly in patients with moderate to severe WML.
    Digital Access Access Options
  • Article
    Zhang L, Wang Y, Pan RL, Li Y, Hu YQ, Xv H, Zhu C, Wang X, Yin JW, Ma KT, Zhao D.
    Exp Cell Res. 2021 10 15;407(2):112832.
    The autophagy/apoptosis interaction has always been a focus of study in pathogenicity models. Neuritin is a neurotrophic factor that is highly expressed primarily in the central nervous system. Our previous study revealed that it protects against apoptosis in cortical neurons subjected to oxygen-glucose deprivation (OGD)/reoxygenation (OGD/R), and later animal experiments revealed that it can increase the expression of the autophagy-related protein LC3. Whether this neuroprotective effect is closely related to autophagy is still unclear. In this study, we hypothesized that neuritin can promote autophagic flux to protect nerve cells after OGD/R. To verify this hypothesis, we induced OGD/R in primary cortical neurons and assessed cell viability by the CCK8 and LDH assays. Cell apoptosis was assessed by Annexin V-FITC/PI, staining, and the contents and mRNA abundances of the autophagy-related proteins LC3 and p62, the apoptotic protein Caspase3 were quantified by Western blotting and RT-PCR. Autophagic flux was assessed by immunofluorescence after RFP-GFP-LC3 virus transfection, and ultrastructural changes in autophagosomes were observed by transmission electron microscopy (TEM). The results showed that cell viability was decreased, apoptosis was increased and autophagy was enhanced after OGD/R. Neuritin significantly increased cell viability, decreased apoptosis, further increased the expression of the autophagic flux-related protein LC3, further decreased p62 expression, and significantly increased the autophagosome number and autophagosome to lysosome ratio. Bafilomycin A1 (BafA1) is a late autophagy inhibitor, aggravated cell damage and apoptosis and counteracted the enhancement of autophagy activation and protective effects of neuritin. In conclusion, neuritin may promote the completion of autophagic flux by ameliorating neuronal damage after OGD/R.
    Digital Access Access Options
  • Article
    Reghukumar C, Shamjith S, Murali VP, Ramya PK, Radhakrishnan KV, Maiti KK.
    J Photochem Photobiol B. 2024 Jan;250:112832.
    The increased energy demands inherent in cancer cells necessitate a dependence on mitochondrial assistance for their proliferation and metastatic activity. Herein, an innovative photo-medical approach has been attempted, specifically targeting mitochondria, the cellular powerhouses, to attain therapeutic benefit. This strategy facilitates the rapid and precise initiation of apoptosis, the programmed cell death process. In this goal, we have synthesized cyclometalated Iridium (III) molecular probes, denoted as Ir-CN and Ir-H, with a nitrile (CN) and a hydrogen-functionalized bipyridine as ancillary ligands, respectively. Ir-CN has shown superior photosensitizing properties and lower dark cytotoxicity compared to Ir-H in the breast cancer cell line MCF-7, positioning it as the preferred probe for photodynamic therapy (PDT). The synthesized Ir-CN induces alterations in mitochondrial membrane potential, disrupting the respiratory chain function, and generating reactive oxygen species that activate signaling pathways leading to cell death. The CN-conjugated bipyridine ligand in Ir-CN contributes to the intense red fluorescence and the positive charge on the central metal atom facilitates specific mitochondrial colocalization (colocalization coefficient of 0.90). Together with this, the Iridium metal, with strong spin-orbit coupling, efficiently generates singlet oxygen with a quantum yield of 0.79. Consequently, the cytotoxic singlet oxygen produced by Ir-CN upon laser exposure disrupts mitochondrial processes, arresting the electron transport chain and energy production, ultimately leading to programmed cell death. This mitochondrial imbalance and apoptotic induction were dually confirmed through various apoptotic assays including Annexin V staining and by mapping the molecular level changes through surface-enhanced Raman spectroscopy (SERS). Therefore, cyclometalated Ir-CN emerges as a promising molecular probe for cancer theranostics, inducing laser-assisted mitochondrial damage, as tracked through bimodal fluorescence and SERS.
    Digital Access Access Options
  • Article
    Qin HL, Zhang ZW, Ravindar L, Rakesh KP.
    Eur J Med Chem. 2020 Dec 01;207:112832.
    The development of drug-resistant bacteria, as well as multidrug-resistant pathogens related to substantial mortality, is an important global health threat. The wide range of biological activities and the wealthy use of coumarin-containing drugs in the clinic have encouraged more and more interest in this class of heterocycles. Various coumarin-based antibiotic hybrids have been developed in the last decade and most of them exhibited potential antibacterial potency. This present review summarizes the current innovations of coumarin-based derivatives with potential antibacterial activities against a variety of Gram-negative and Gram-positive bacteria discussing various aspects of structure-activity relationship (SAR). The improved SAR will provide further insight into the rational improvement of coumarin hybrids with astounding strength against multidrug-resistant bacteria.
    Digital Access Access Options
  • Article
    Gabriunaite I, Valiūnienė A, Poderyte M, Ramanavicius A.
    J Pharm Biomed Anal. 2020 Jan 05;177:112832.
    Recently, self-assembled monolayers (SAMs) are gaining a lot of interest due to their simplicity of preparation and wide applicability in the development of model systems used in pharmaceutical and biomedical analysis. The most efficient methods used for the investigation of SAM-based structures usually include cyclic voltammetry and electrochemical impedance spectroscopy. Scanning electrochemical microscopy (SECM) could be also used as an alternative method for SAM investigations, because this method enables to map modified surface. In this work, the surface of fluorine doped tin oxide (FTO) was modified with octadeciltrichlorosilane (OTS) based SAM and investigated using SECM. Measurements, which were carried out by SECM, lead to conclusion that highly heterogeneous and distributed monolayer has been formed on FTO surface.
    Digital Access Access Options
  • Article
    Zhou Y, Zhang M, Zhao X, Feng J.
    Ecotoxicol Environ Saf. 2021 Dec 15;226:112832.
    Ammonia is a known environmental pollutant that causes injury to the intestine. Growing evidence suggests that intestinal microbiota dysbiosis involves in the development of intestinal injury under environmental pollution. However, the specific mechanism remains unexplored. To do this, broiler chicken ileal exposed to ammonia was selected as the research object. Further, antibiotic depletion of intestinal microbiota and flora transplantation were used to clarify the role of intestinal microbiota in the intestinal injury. Histopathological examination indicated inhaled ammonia caused intestinal injury. Then we observed a decrease in intestinal muc-2, claudin-1, IL-6, IL-10 in ammonia inhalation, as opposed to the control group, associated with a significant increase in TLR4, MyD88, NF-κB, TNF-α, IL-1β, caspase3. Moreover, there was a significant increase of Streptococcus, Escherichia-Shigella, Faecalibacterium, [Ruminococcus]_torques_group, Ruminococcaceae_UCG-014, unclassified_f_Lachnospiraceae, Rothia, unclassified_f_Ruminococcaceae in the inhaled ammonia exposure. Correlation analysis suggested that the altered genera were positively correlated with the expression of TLR4 and TNF-α. Moreover, transferring intestinal microbiota from ammonia exposure broiler into healthy broiler caused intestinal injury and increased TLR4 and TNF-α concentrations in recipient broiler. Furthermore, antibiotic depletion of intestinal microbiota attenuated ammonia-caused intestinal injury and reduced TLR4 and TNF-α productions. In summary, TLR4/TNF-α signaling pathway was an important regulated mechanism involved in the intestinal injury mediated by intestinal microbiota dysbiosis under inhaled ammonia.
    Digital Access Access Options
doi:10.1016/j.ultramic.2019.112832 doi:10.1016/j.celrep.2023.112832 doi:10.1016/j.envres.2022.112832 doi:10.1016/j.biopha.2022.112832 doi:10.1016/j.intimp.2024.112832 doi:10.1016/j.physbeh.2020.112832 doi:10.1016/j.foodres.2023.112832 doi:10.1016/j.jep.2020.112832 doi:10.1016/j.bbr.2020.112832 doi:10.1016/j.psychres.2020.112832 doi:10.1016/j.colsurfb.2022.112832 doi:10.1016/j.bios.2020.112832 doi:10.1016/j.jinorgbio.2025.112832 doi:10.5812/aapm.112832 doi:10.1371/journal.pone.0112832 doi:10.1016/j.yexcr.2021.112832 doi:10.1016/j.jphotobiol.2023.112832 doi:10.1016/j.ejmech.2020.112832 doi:10.1016/j.jpba.2019.112832 doi:10.1016/j.ecoenv.2021.112832