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  • Book
    edited by Frederic Devaux.
    Contents:
    Using RNA-seq for analysis of differential gene expression in fungal species / Can Wang ... [et al.]
    Enhancing structural annotation of yeast genomes with RNA-Seq data / Hugo Devillers, Nicolas Morin, and Cécile Neuvéglise
    Pathogen gene expression profiling during infection using a nanostring nCounter platform / Wenjie Xu ... [et al.]
    Comparative transcriptomics in yeasts / Dawn A. Thompson
    Mapping the transcriptome-wide landscape of RBP binding sites using gPAR-CLIP-seq : experimental procedures / Ting Han and John K. Kim
    Mapping the transcriptome-wide landscape of RBP binding sites using gPAR-CLIP-seq : bioinformatic analysis / Mallory A. Freeberg and John K. Kim
    Translation analysis at the genome scale by ribosome profiling / Agnès Baudin-Baillieu ... [et al. ]
    Biotin-genomic run-on (Bio-GRO) : a high-resolution method for the analysis of nascent transcription in yeast / Antonio Jordán-Pla ... [et al.]
    Genome-wide probing of RNA structures in vitro using nucleases and deep sequencing / Yue Wan ... [et al.]
    Genome-wide chromatin immunoprecipitation in Candida albicans and other yeasts / Matthew B. Lohse [et al.]
    ChIPseq in yeast species : from chromatin immunoprecipitation to high-throughput sequencing and bioinformatics data analyses / Gaëlle Lelandais, Corinne Blugeon, and Jawad Merhej
    Systematic determination of transcription factor DNA- binding specificities in yeast / Lourdes Peña-Castillo and Gwenael Badis
    Generation and analysis of chromosomal contact maps of yeast species / Axel Cournac ... [et al.]
    Versatile procedure to generate genome-wide spatiotemporal program of replication in yeast species / Nicolas Agier and Gilles Fischer
    Single-step affinity purification (ssAP) and mass spectrometry of macromolecular complexes in the yeast S. cerevisiae / Christian Trahan, Lisbeth-Carolina Aguilar, and Marlene Oeffinger
    Label-free quantitative proteomics in yeast / Thibaut Léger ... [et al.]
    Profiling of yeast lipids by shotgun lipidomics / Christian Klose and Kirill Tarasov
    Identification of links between cellular pathways by genetic interaction mapping (GIM) / Christophe Malabat and Cosmin Saveanu
    On the mapping of epistatic genetic interactions in natural isolates : combining classical genetics and genomics / Jing Hou and Joseph Schacherer
    Experimental evolution and resequencing analysis of yeast / Celia Payen and Maitreya J. Dunham
    Reconstruction and analysis of the evolution of modular transcriptional regulatory programs using arboretum / Sara A. Knaack, Dawn A. Thompson, and Sushmita Roy
    Predicting gene and genomic regulation in Saccharomyces cerevisiae, using the YEASTRACT database : a step-by-step guided analysis / Miguel C. Teixeira, Pedro T. Monteiro, and Isabel Sá-Correia.
    Digital Access Springer 2015
  • Article
    Saarem K, Pedersen JI, Tollersrud S.
    Comp Biochem Physiol B. 1978;61(4):485-90.
    1. Mitochondria isolated from the kidneys of rachitic pigs have been shown to contain an active 25-hydroxyvitamin D3-1 alpha-hydroxylase. From these mitochondria a cytochrome P-450 has been solubilized with a specific content of 0.02-0.04 nmol/mg protein. 2. In the presence of a bovine adrenal NADPH-ferredoxin reductase, bovine adrenal ferredoxin and NADPH, the cytochrome P-450 supported the formation of 1,25-dihydroxyvitamin D3 from 25-hydroxyvitamin D3. 3. The hydroxylation reaction was linear with time up to 40 min, and with the amount of enzyme up to 0.03 nmol cytochrome P-450. The pH optimum for the reaction was 7.4, and the apparent Km was 3 x 10(-10) mol/mg protein. 4. The results show that 25-hydroxyvitamin D3 is metabolized in mammals by the same enzyme system as has been demonstrated in birds.
    Digital Access Access Options