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- Bookedited by David Biron, Gal Haspel.Contents:
Library construction for mutation identification by whole-genome sequencing / Harold E. Smith
Fundamentals of comparative genome analysis in caenorhabditis nematodes / Eric S. Haag and Cristel G. Thomas
Genetic methods for cellular manipulations in C. elegans / Menachem Katz
Fusion PCR method for expressing genetic tools in C. elegans / Yifat Eliezer and Alon Zaslaver
Transposon-assisted genetic engineering with Mos1- mediated single-copy insertion (MosSCI) / Christian Frøkjær-Jensen
Creating genome modifications in C. elegans using the CRISPR/Cas9 system / John A. Calarco and Ari E. Friedland
Observing and quantifying fluorescent reporters / Michael Hendricks
Microbial rhodopsin optogenetic tools : application for analyses of synaptic transmission and of neuronal network activity in behavior / Caspar Glock, Jatin Nagpal, and Alexander Gottschalk
Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans / Nicholas F. Trojanowski and Christopher Fang-Yen
High-pressure freeze and freeze substitution electron microscopy in C. elegans / Laura Manning and Janet Richmond
Electron tomography methods for C. elegans / David H. Hall and William J. Rice
Microfluidic devices for behavioral analysis, microscopy, and neuronal imaging in Caenorhabditis elegans / Ross C. Lagoy and Dirk R. Albrecht
Tracking single C. elegans using a USB microscope on a motorized stage / Eviatar I. Yemini and André E. X. Brown
Imaging system for C. elegans behavior / Matthew A. Churgin and Christopher Fang-Yen
Method for obtaining large populations of synchronized Caenorhabditis elegans dauer larvae / Maria C. Ow and Sarah E. Hall
Sampling and isolation of C. elegans from the natural habitat / Nausicaa Poullet and Christian Braendle
Primer on prototyping / Dylan Lynch and David Biron
Primer on quantitative modeling / Iulia Neagu and Erel Levine.Digital Access Springer 2015 - ArticleSachs HG, Colgan JA, Lazarus ML.Comp Biochem Physiol B. 1978;61(1):181-3.1. Mitochondria isolated from the ventricles of Cavia porcellus, Mesocricetus auratus, Rattus rattus and Mus musculus accumulate calcium in an energy-linked process. 2. Measurements of the initial velocity of accumulation by dual-wavelength spectrophotometry show species-dependent variation, with Cavia demonstrating the fastest rate and Rattus the slowest rate of accumulation. 3. Total amount of calcium accumulated per unit protein varied in a manner similar to rate of accumulation, with Cavia accumulating the greatest amount of calcium and Rattus the least.